Factors affecting Enzyme Action

Question 1

How can you measure rate of change at a point on a graph

Answer 1

Find gradient
Change in Y / Change in X

Question 2

How does optimal temperature affect enzyme action

Answer 2

As temp rises, kinetic energy increases leading to more more collisions between the enzyme and the substrate resulting in more enzyme-substrate complex being formed

Question 3

How does moderately high temperature affect enzyme action

Answer 3

As temperature rises past optimal, the hydrogen bonds and other bonds in the active site of the enzyme begin to change shape, leading to the substrate fitting less easily in the enzyme causing a decrease in rate of reaction

Question 4

How does a high temperature affect rate of enzyme action

Answer 4

At high temperatures the enzyme bonds in the active site break cause it to become denatured. This is a permanent change and means the enzyme - substrate complex cannot be formed, stopping the reaction.

Question 5

Effect of pH change on enzyme action

Answer 5

A change in pH alters the charges on the amino acids on the active site of the enzyme, meaning the substrate can no longer attach to the active site so the enzyme substrate complex cannot be formed

Question 6

Effect of enzyme concentration on rate of reaction

Answer 6

When increasing enzyme concentration, more collisions occur between enzymes and substrates leading to more enzyme substrate complexes being formed resulting in an increase in rate of reaction.
When there are more enzymes than substrates , rate of reaction plateaus.