5.5 Translation Flashcards
What is translation?
when mRNA is used to synethesize a polypeptide chain that will make a functional protein
What is the site of translation?
ribosome
Where does transcription take place and where does translation take place?
transcription - nucleus
translation - cytoplasm
What is tRNA?
transfer RNA
What are the two regions of tRNA?
- anticodon region that is complementary to the mRNA
- region that is covalently bound to the correct amino acid that matches the codon
What are the two subunits of the ribosome?
- large
- small
What is each subunit of the ribosome composed of?
each is composed of rRNA and ribosomal proteins
What is a monosome?
each subunit of the ribosome composed of rRNA and ribosomal proteins
How are ribosomes measured?
characterized based on the rate of migration in a sucrose gradient known as the Svedberg coefficient
What does the Svedberg coefficient take into account?
density, mass, and shape of a ribosome
What is the bacterial monosome and what is the eukaryotic monosome?
bacterial = 70s
eukaryotic = 80s
What is the bacterial 70s monosome composed of?
50s and 30s
What is the eukaryotic 80s composed of?
60s and 40s
How big are tRNAs?
small; 70-90 nucleotides long
What is posttranscriptional modification?
enzymatic reaction occurs after the transcription of RNA