5.4 Regulation of Transcription Flashcards

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1
Q

What is the process of transcription?

A

conversion of genetic material in DNA to RNA

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2
Q

What is the outcome of transcription?

A

messenger RNA

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3
Q

What does it mean to be complimentary?

A

for each base, the letter that forms the corresponding hydrogen bonds will be made

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4
Q

Where does translation into proteins occur?

A

ribosomes in the cytoplasm

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5
Q

What enzyme is reponsible for the synthesis of DNA to RNA?

A

RNA polymerase

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6
Q

Is primer required for initiation of RNA polymerase?

A

no

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7
Q

In E coli what is RNA polymerase made of?

A

core enzyme and the sigma unit

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8
Q

What is the core enzyme?

A

comprised of four subunits

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9
Q

What is the holoenzyme?

A

the core enzyme and the sigma unit together

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10
Q

How many RNA polymerases are in eukaryotes?

A

three

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11
Q

Which strand does transcription occur on?

A

the template strand

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12
Q

What is the other strand known as? (not the template one)

A

coding strand

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13
Q

Which strand has an identical sequence to the mRNA that is being sequenced?

A

coding strand

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14
Q

What is the first step in bacterial gene transcription?

A

template binding

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15
Q

What occurs during template bindning?

A

sigma subunit of RNA polymerase recognizes a promoter

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16
Q

What is the promoter?

A

specific DNA sequence that starts a gene

17
Q

Where is the promoter located in relation to the rest of the gene sequence?

A

upstream

18
Q

What does it mean to be upstream?

A

5’ region

19
Q

What does denatured mean?

A

DNA is unwound

20
Q

What is the position +1 also known as?

A

transcription start site