5.2 DNA Replication Part 2 Flashcards
What is the function of DNA poly I?
remove the primer and fill in gaps left behind
Where does DNA initiation occr in eukaroytic replication?
origin of replication
How are DNA strands polymerized in eukaryotic replication?
template/primer DNA strand
What are the main differences between eukaryotic and prokaryotic DNA replication?
eukaryotic is
1. more complex
2. condensed with proteins
3. linear rather than circular
How much slower does DNA polymerases synthesize eukaryotic DNA as compared to prokaryotic?
25 times slower
What is used to increase the efficiency of DNA replication in eukaryotes?
there are multiple origins of replication
How are the origins of replication visible?
as bubbles using an electron microscope
What is the origin recognition complex?
six protein complex that marks the site of origin of replication
When does the ORC do its job?
during G1 of the cell cycle
What is the prereplication complex?
recruitment of 20 others proteins to the ORC to signal DNA polymerase to start synthesis
What allows DNA replication to occur at multiple places at once but still remaining orderly and efficient?
keeping the prereplication complexes delineated
What is the function of kinases?
phosphorylate replication proteins to activate them
What is the function of helicases?
unwind double stranded DNA
Once the pre-RC is formed what else is recruited to the replication fork?
kinases and helicase
How many different DNA polymerases are in eukaryotes?
14
