3.1 DNA Structure and Evidence as Genetic Material Flashcards

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1
Q

What year did it become clear that DNA was the basis for an individuals genetic make up?

A

1944

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2
Q

What are some “things” that do not have DNA as that basic building block of existence?

A

unique viruses

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3
Q

Who discovered the structure of DNA and when?

A

Watson and Crick; 1953

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4
Q

What did Watson and Crick determine about DNA structure?

A

double-helical structure comprised of two strands of DNA containing long chains of nucleotides

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5
Q

When did Watson and Crick win the Nobel Prize?

A

1962

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6
Q

When was DNA actually discovered and by whom?

A

1869; Miescher

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7
Q

What did Miescher identify?

A

nuclein within the nuclei of human white blood cells

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8
Q

What was Levene’s theory and what was the year?

A

1910; DNA contains equal amounts of four molecules called nucleotides which are present in repetitive and equal amounts in DNA

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9
Q

What was Levene wrong about?

A

that nucleotides were repetitive and in equal amounts

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10
Q

What are the four characteristics that the genetic flow of material between organisms is dependent on?

A
  1. replication of materials
  2. storage of information
  3. expression of information
  4. variation through mutations
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11
Q

How is genetic material doubled and passed along?

A

division of daughter cells (mitosis) and gametes (meiosis)

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12
Q

Where is genetic material stored?

A

nucleus

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13
Q

What are sources of variation within a population?

A

mutations

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14
Q

What did Griffith note in his experiments and when?

A

1927; some strains of D. pneumoniae were virulent and some were not

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15
Q

What determined virulence in Griffith’s experiment?

A

virulent strains contained a polysaccharide capsule while avirulent did not

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16
Q

What did the capsule do in Griffith’s experiment?

A

protected the virulent strains from being engulfed by phagocytic immune cells in the host’s circulatory system

17
Q

When plated on agar plates, what did the virulent and avirulent strains of Griffith’s experiment produce?

A

virulent = shiny, smooth colonies
avirulent = rough colonies

18
Q

What two serotypes did Griffith use in his experiment?

A

IIR = rough, no capsule, avirulent
IIS = smooth, capsule, virulent

19
Q

What were the three things Griffith noted in his experiment?

A
  1. injecting live avirulent IIR into mice produced no disease
  2. injecting heat-killed virulent IIIS into mice produced no disease
  3. injecting live avirulent IIR WITH heat-killed IIIS killed all the mice
20
Q

What process did Griffith discover with his experiment with D. pneumoniae?

A

transformation