5.2 DNA Replication Flashcards
What are the three phases of how a gene is turned into a protein?
DNA replication
transcription
translation
What allows DNAto serve as a template for synthesis of two more complimentary strands?
complementarity of the DNA double helix
What does each round of DNA replication consist of?
one old strand and one new strand
What is semiconservative replication?
the process of mixing and old strand with a new strand during DNA replication
When did researchers test conservative replication?
1957
What is conservative replication?
when two parent strands make two new strands but then come back together
What was the experiment in 1957 that tested conservative replication?
use of faba bean plant to monitor DNA replication using DNA labeling with 3 H-thymidine
What is the replication fork?
chromosomal location where DNA replication starts in bacteria
Where does the replication fork appear in bacteria?
origin of replication (oriC)
What did studies with bacteriophage lambda show for DNA replication?
that it is bidirectional at the replication fork
Which bacteria is the easiest to culture on both plates and in liquid culture?
E coli
What was the experiment conducted by Arther Kornberg?
isolating a specific enzyme from E coli that could directly synthesize DNA in vitro
What was the enzyme that Kornberg managed to isolate from E coli?
DNA polymerase I
What is required to synthesize DNA in a test tube?
DNA polymerase I
template DNA
all four dNTPs
Which direction is DNA synthesized in?
5-3
