17 - Bacterial Pathogenesis Flashcards

1
Q

Bacteria which commonly have afimbrial adhesins

A

G- bacteria (as are often inserted in outer cell membrane)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Proportion of microbiome that is in large intestine

A

~70%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Protein that ETEC uses to bind to human intestinal epithelium

A

CS3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Experiment looking at ETEC virulence in piglets

A

Without K88 adhesin, there was no ETEC adhesion to intestinal wall, an no pathogenesis, even in E coli that were toxin+ (no diarrhoea)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

How can piglets be immunised?

A

Immunise sow, when piglets suckle milk get sow’s IgA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Travelan

A

Antibodies against ETEC.

Treats traveller’s diarrhoea

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Where do yersinia enter intestinal wall?

A

Through M cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Which protein does yersinia use to enter M cells?

A

Invasin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What does yersinia invasin bind to?

A

Integrin on M cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What are pyogenic bacteria?

A

Pathogenic bacteria that evade phagocytosis, resulting in pus formation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Example of bacterium which produces leukocidins

A

S pyogenes, S aureus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Example of a bacterium that produces an anti-inflammatory

A

Choleratoxin is an anti-inflammatory agent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Number of S. pneumoniae capsular antigens

A

Around 90

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Is there any cross-reactivity between Ig against S. pyogenes capsules?

A

No

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Adult pneumococcal vaccine

A

23 most-common S. pyogenes capsular antigens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Children’s’ pneumococcal vaccine

A

13 most-common S. pyogenes capsular antigens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

How can capsulated bacteria be opsonised?

A

Anti-capsular antigen Ig binds, and complement binds to Ig Fc region

18
Q

Most important type of meningococcus worldwide

A

Type A

19
Q

Most important type of meningococcus in Australia

A

Type B

20
Q

Example of a bacterium with an non-antigenic capsule

A

Meningococcus type B

21
Q
Stages of phagocytosis
1)
2)
3)
4)
5)
6)
A

1) Chemotaxis
2) Attachment
3) Metabolic burst
4) Ingestion
5) Phagolysosome formation
6) Destruction

22
Q
Some strategies used by bacteria to evade destruction by phagocytes
1)
2)
3)
4)
A

1) Inhibit respiratory burst
2) Prevent phagolysosome formation
3) Escape from phagocytic vacuole
4) Resist bactericidal systems

23
Q

C’ pathway activated by LPS

A

Alternative pathway

24
Q

Non-professional phagocytes

A

Cells that need to be induced to plagocytose.

EG: With SIP proteins

25
Q

Substrate of lysozyme

A

Beta1-4 linkage in peptidoglycan

26
Q

Why are newborn babies a risk group for tetanus infection?

A

Some cultures cover necrotising umbilical cord with cow dung. C tetani lives in cow faeces (and faeces of other animals), colonises necrotising tissue.

27
Q

Only way that you can get anti-tetanustoxin Ig

A

Immunisation. The amount of tetanospasmin required for Ig production in a natural infection would kill you.

28
Q

How can replicating bacteria release endotoxin?

A

Some G- bacteria release bits of cell wall

29
Q

Pyrogen free

A

Autoclaving won’t inactivate endotoxin. Pyrogen free means endotoxin has been removed

30
Q

Heat resistance of endotoxin

A

High

31
Q
Ways to overcome adaptive immune system
1)
2)
3)
4)
A

1) Direct immunosuppression
2) Expression of weak antigens
3) Antigenic diversity
4) Antigen modification

32
Q

Chemical nature of exotoxin

A

Protein

33
Q

Chemical nature of endotoxin

A

LPS

34
Q

Antigenicity of exotoxin versus endotoxin

A

Exotoxin highly antigenic. Endotoxin variable

35
Q

Specificity of exotoxin versus endotoxin

A

Exotoxin very specific. Endotoxin non-specific (as induces immune response)

36
Q

Broad division of bacterial exotoxin modes of action

A

1) Cytotoxic (inhibitory), EG leukocidins

2) Cytotonic (excitatory), EG choleratoxin

37
Q

Examples of intracellularly acting, cytotoxic exotoxins
1)
2)

A

1) Diphtheriatoxin

2) Shigatoxin

38
Q

Example of a simple exotoxin

A

Heat-stable enterotoxin

39
Q

Shigatoxin receptor

A

gb3

40
Q

SHigatoxin mechanism

A

A1 subunit modifies 28S rRNA of 60S ribosomal subunit, prevents protein synthesis