16 - B Cells Flashcards
Gene segments involved in heavy chain reassortment
V D J
Gene segments involved in light chain reassortment
V J (kappa or lambda)
Enzyme that is involved in minigene recombination
RAG (recombination activation gene)
4 main sources of antibody diversity
1) Junctional diversity
2) Combinatorial diversity
3) V region diversity
4) Somatic hypermutation
Factor that contributes the most to BCR diversity
Junctional diversity
Enzyme that increases junctional diversity
TdT (terminal deoxynucleotide transferase)
Important enzyme in testing of pre-BCR
Bruton’s tyrosine kinase. Involved in signal transduction from binding of pre-BCR to ligand
Important interleukin in lymphocyte development
IL-7 (inhibits lymphocyte apoptosis)
What releases IL-7 in lymphocyte development?
Bone marrow stromal cells
Early pro-B cell
DJ heavy chain rearrangement
Late pro-B cell
V-DJ heavy chain rearrangement
Large pre-B cell
Heavy chain VDJ rearranged.
Complex heavy chain with pseudo light chain to test if functional. BTK necessary for detection of binding.
Small pre-B cell
V-J light chain rearrangement
Immature B cell
IgM expressed on cell surface
Mature B cell
IgM and IgD expressed on cell surface from differential splicing of heavy chain transcripts
