XII: Chapter 12- Biotechnology and its application Flashcards
Give examples of lepidopterans.
Tobacco budworm, armyworm
Proteins coded by which genes control cotton bollworms?
Cry1Ac and Cry2Ab
Proteins coded by which gene control corn borers?
Cry1Ab
How were nematode specific genes introduced into the host plant?
Using agrobacterium vectors
How many therapeutics have been approved for human use worldwide and in India?
30 worldwide and 12 in India
When was genetically engineered insulin first produced?
1983
When was the first clinical gene therapy given?
1990
ADA deficiency stands for
Adenosine deaminase deficiency
As a first step towards gene therapy which cells of the ADA deficiency patient were cultured?
Lymphocytes
How was ADA cDNA introduced into cultured lymphocytes for gene therapy of ADA deficiency?
Using retroviral vector
No transgenic model exists for cystic fibrosis. True/false?
False. Transgenic models exist for cystic fibrosis.
No transgenic model exists for Alzheimer’s. True/false?
False. Transgenic models exist for Alzeihmer’s.
When was the first transgenic cow made?
1997
How many gram of protein was present per litre in the milk produced by Rosie the transgenic cow?
2.4g
GEAC stands for
Genetic engineering approval committee
In which year did an American company get patent rights on Basmati rice through the US Patent and trademark office?
1997
How many amino acids does insulin have in both of its chains?
A- chain : 21 amino acids
B-chain : 30 amino acids
What are second generation vaccines?
rDNA vaccines
What are third generation vaccines?
DNA vaccines
What is the use of interferon alpha therapeutic?
Treatment of cancer
What is the use of tissue plasminogen activator therapeutic?
Myocardial infarction
What is the use of the therapeutic DNAse?
Cystic fibrosis
What is the use of the therapeutic OKT-3?
immunosuppressive agent to prevent acute rejection in case of organ transplant
What is the use of the therapeutic GCSF?
Cancer treatment
What is the blood tested for in direct ELISA?
Antigen
What is attached to the well in direct ELISA?
Enzyme linked antibody
What is the blood tested for in Indirect ELISA?
Antibody
What is present on walls of wells in indirect ELISA?
Antigen
What is the blood tested for in sandwich ELISA?
Antigen
What is attached on the walls of wells in sandwich ELISA?
Primary antibody
Which part of the insect does Bt toxin affect?
Epithelial cells of midgut
Transgenic golden rice is made by the addition of which gene in Oryza sativa?
Beta-carotene producing gene from daffodils or carrots
Which plant was genetically engineered to make hirudin?
Brassica napus
RNA interference is present in all organisms as a method of cellular defence. True/false?
False.
RNA interference is present in all eukaryotes as a method of cellular defence.
Which was the first transgenic plant available in market?
Flavr savr tomato
Golden rice is rich in
Vitamin A and iron
What are the qualities of flavr savr tomatoes which make them different from other tomatoes?
Longer shelf life, stronger taste, bigger
What is the function of genetically modified pseudomonas florescence?
Prevents frost damage on the plants that it grows
What is the super bug?
Pseudomonas putida
Scavenger of oil spills
HGH insert in common carp makes it
2 times in size
Name the first transgenic sheep.
Polly molly
Name the first successful mammalian clone
Dolly the sheep
To create first transgenic sheep, the host was
Black faced ewe
To create first transgenic sheep the host was
White faced ewe
