transcription Flashcards
what happens in transcription
- messenger rna is produced in nucleus from a template of a gene coding region of dna
- mrna leaves nucleus to join ribosome (in cytoplasm)
initiation
rna polymerase bonds to promotor region of DNA
unzips the dna
promotor and terminator region
promotor: is a base sequence that signals the beginning of a gene, the strand to be copied and direction.
elongation
- free rna nucleotides join with complementary DNA nucleutides ( a-u)
- RNA polymerase creates a covalent bond between nucleotides, creating a strand of pre-mrna.
termination
- RNA polymerase adds bases to the rna chain until it reaches termination region of dna
- the single stranded pre-mrna strand detaches from the DNA and the two strands of DNA region
introns and exons
certain sequences are non-coding
introns are nucleotides not needed, it is spliced/removed.
exons do code for amino acid sequence
cap and poly-A tail
to protect mrna from being cut apart in the cell, a cap is added in the 5’ and a poly A tail is added in the 3’
movement out of the nucleus
DNA code is now carried by single stranded mrna molecule (made of exons) out of the nucleus, through a nuclear pole, to the ribosomes,
Direction of new rna nucleotides
new rna nucleotides added in 5’ to 3’ direction (templaate dna reads 3 to 5)
Similarities between template dna strand and mrna
the base sequence in the mrna transcript is complementary to the sequence in the dna template strand.