polymerase chain reaction PCR Flashcards
1
Q
why do people do it
A
allow biologist to make copies of genes.
small amounts of DNA can be multiplied making it easier to analyze
2
Q
DNA denaturing
A
always the highest temperature
temp is increased to separate DNA strands
3
Q
annealing
A
temp is decreased to allow primers to base pair to complementary DNA template.
4
Q
extension
A
temp increased (but not as high as before)
activates polymerase, it locates primer, and adds complementary bp onto strands
