Topic 53 Flashcards
Methods of parentage control
blood groups and DNA allele polymorphisms
Polymorphism
two or more phenotypes existing in the same population
Why is polymorphism important for parentage control?
Because of selective mating and estimation of relationship
level of differences - phenotype
not always visible; morphological; serological: blood groups, HA; biochemical: proteins and enzyme polymorphisms
Parentage control with blood groups
used in wide range; cheap, fast, accurate; blood group antigen; simple codominant inheritance; 90% certainty
Blood group antigen
generally determined antigen set on surface of RBC inherited from parents. All animals inherit antigen only from parents
DNA polymorphisms
there are two or more alleles in the population with notable frequencies
DNA polymorphism examination strategies
RFLP, PCR
RFLP
Restriction fraction length polymorphisms
PCR
Polymerase chain reaction used to compare offspring to parent DNA