Topic 49

Question 1

OPU

Answer 1

Ovum Pick Up - collection of ova by sonogram

Question 2

IVM

Answer 2

In vitro maturation of collected ova

Question 3

IVF

Answer 3

In vitro fertilization of mature ova - swim up method

Question 4

Advantages

Answer 4

Successful method in many species;humans, swine, cattle, rat, mouse; generation interval, may be shortened; gene transfer possible in early embryonic phase; sexing can be performed on early embryo; detection of SRY gene

Question 5

How many pregnancys can one cow produce?

Answer 5

50-100

Question 6

In vitro

Answer 6

Oocytes are harvested and matured and fertilized

Question 7

How many times a week can they be flushed?

Answer 7

At least twice a week

Question 8

Oocyte deepfreezing

Answer 8

Slow freezing; Ultrarapid freezing

Question 9

Slow freezing (oocyte)

Answer 9

in cattle, sheep , low success rates, high wmbryo mortality following fertilization, need highly qualified technician

Question 10

Ultrarapid freezing (oocyte)

Answer 10

(vitrification) limits damage to oocyte

Question 11

Embryo deep freezing

Answer 11

slow freezing; fast freezing

Question 12

Slow freezing (embryo)

Answer 12

limits the rupture of the membrane due to intracellular ice formation. Don’t need to remove cryoprotectant when implanting into recipient. Used in cattle mostly

Question 13

Fast freezing (embryo)

Answer 13

ultra rapid cooling (vitrification) in very small amount of cyroprotectant