Topic 50

Question 1

MOET

Answer 1

Multiplex Ovulation and Embryo Transfer

Question 2

Advantages of MOET

Answer 2

Improve reproduction potential, selection intensity, breeding value, progeny testing; Possibility from uniporous to pluriporous sp. (litter) in Ru; Induction of twinning in beef cattle, by flushing, induction of poly and superovulation, eventually by cloning

Question 3

ET

Answer 3

Embryo Transfer

Question 4

Embryo Transfer

Answer 4

Careful section of donor and recipient animals; Cycle synchronization in recipient and superovlation in donor by given hormones; AI of superovulated dam; Flushing of embryo from uterus with Foley catheter; Evaluation and selection of embryos: cutting of non viable embryos (deep freezing); Embryo transfer using catheter - not surgical, transcervical in sheep, horse. Surgical in goat and swine

Question 5

What are clones?

Answer 5

Genetically identical individuals

Question 6

Where are clones common?

Answer 6

Common in nature - asexual reproduction of plants and microorganisms; In mammals like armadillos and monozygous twins

Question 7

What is the concern with cloning?

Answer 7

Food safety concern

Question 8

First mammalian clone?

Answer 8

Dolly created from the nucleus of an adult mammary cell

Question 9

Methods of cloning?

Answer 9

Embryo splitting (full genetic identify, they are called as embryonic clones); Transfer of embryonic or somatic cell nucleus into zona pellucida of ova. Not 100% due to mtDNA

Question 10

Notes from exam

Answer 10

Technically not possible to have 100% clone; Armadillo has either 4, 8, or 12 offspring - begins at 4 and then they divide

Question 11

embryo flushing

Answer 11

part of embryo transfer

• FSH -> multiple eggs(ovulation) -> AI (artificial insamination)
• after fertilization the eggs are flushed (collected) from the cows uterus)

then straight into another cow OR freezed for later use