TLRs

Question 1

Gram + bacteria are detected by what receptor?

Answer 1

TLR2, TLR6

Question 2

Lipoproteins are detected by what receptor?

Answer 2

TLR2

Question 3

TLR2 detects

Answer 3

ROS, peptidoglycans, liptoteichoic acid (Gram positive bacteria)

Question 4

TLR4 detects

Answer 4

ROS, lipopolysaccharides (Gram negative)

Question 5

Gram negative bacteria are detected by

Answer 5

TLR4

Question 6

TLRs are located

Answer 6

on cell surface and in endosomes

detect microbial Ags (LPS, peptidoglycans, viral nucleic acids)

Question 7

TLRs possess what kind of structure, recognize what classes of molecules, and what kinds are expressed according to location in the cell

Answer 7

homodimers or heterodimers

PAMPs and DAMPs

1,2,4,5,6 located on the surface

3,7,8, 9 located in endosomes

Question 8

TLRs: 1, 2, 5, 6 use

Answer 8

adaptor MyD88 to activate transcription factors NF-kB and AP-1

Question 9

TRIF is an adaptor protein corresponding to ___ and activates

Answer 9

TLR3, activates IRF3/ IRF7

Question 10

TLR4

Answer 10

can use both TRIF or MyD88 pathways to activate NF-kB/AP1 or IRF3/7

Question 11

What kind of cells express TLR

Answer 11

innate immunity: macrophages and dendrites

Question 12

Bacterial DNA sequences containing unmethylated sequences of CpGs are detected

Answer 12

TLR9

Question 13

ALL TLRs signal through

Answer 13

MyD88 except TLR3

Question 14

TLR4 and TLR2 also engage

Answer 14

TIRAP, which activates IRAK, TRAF, and MAPK or NF-kB

Question 15

NF-kB is the transcription factor for

Answer 15

Acute inflammation and stimulation of adaptive immunity

cytokines IL 1, IL 6, TNF
chemokines
E selectin
costimulatory molecules

Question 16

IRFs are the transcription factors for

Answer 16

antiviral products

Expression of type I interferon (IFN alpha beta)

Question 17

IL-12

Answer 17

controls adaptive T cell immune response