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mt > STAINS IN HP - PART 1 > Flashcards

STAINS IN HP - PART 1 Flashcards

1
Q

Highman’s Congo Red Method for Amyloid: Amyloid

A

Deep Pink to Red

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2
Q

Highman’s Congo Red Method for Amyloid: Nuclei

A

Blue

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3
Q

Alkaline Congo Red Technique: Amyloid, Elastic Fibers, Eosinophil Granules

A

Red

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4
Q

Krajian’s Amyloid Stain (mod. Bennhold method): Amyloid

A

Red on a clear background

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5
Q

Krajian’s Amyloid Stain (mod. Bennhold method): Nuclei

A

Blue

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6
Q

Methyl Violet - Crystal Violet Method: Amyloid

A

Purplish Red

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7
Q

Methyl Violet - Crystal Violet Method: Nuclei, Cytoplasm, CT

A

shade of violet

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8
Q

Mod. Gomori’s Trichome Stain: Muscle Fiber

A

Red

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9
Q

Mod. Gomori’s Trichome Stain: Collagen

A

Green

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10
Q

Mod. Gomori’s Trichome Stain: Nuclei

A

Blue to Black

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11
Q

Mallory’s PTAH: Muscle, Neuroglia

A

Blue

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12
Q

Mallory’s PTAH: Myelin

A

Blue to Blue-Gray

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13
Q

Mallory’s PTAH: Collagen

A

Reddish

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14
Q

Mallory’s PTAH: Coarse Elastic Fibers

A

Bluish

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15
Q

Mallory’s PTAH: Fibrin

A

Blue

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16
Q

Mallory’s PTAH: Nuclei

A

Deep Blue

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17
Q

Heidenhain’s Iron Hematoxylin: Muscle Striations, Mitochondria, Myelin and Chromatin

A

Grey to Black

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18
Q

Lissamin fast red - tartrazine method for muscles and bones: Muscles, Rbcs

A

Red

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19
Q

Lissamin fast red - tartrazine method for muscles and bones: Collagen

A

Yellow

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20
Q

Lissamin fast red - tartrazine method for muscles and bones: Nuclei

A

Black

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21
Q

Schmorl’s picro-thionin method: Lacunae, Canaliculi

A

Dark brown-black

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22
Q

Schmorl’s picro-thionin method: Bone Matrix

A

yellow or brownish yellow

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23
Q

Schmorl’s picro-thionin method: cells

A

Red

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24
Q

Rapid toluidine - eosin stain for glycol-methacrylate sections: Eosinophhilic granules

A

Red

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25
Q

Rapid toluidine - eosin stain for glycol-methacrylate sections: Basophilic and Mast Cell Granules

A

Blue

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26
Q

Rapid toluidine - eosin stain for glycol-methacrylate sections: Nuclei

A

Blue

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27
Q

Rapid toluidine - eosin stain for glycol-methacrylate sections: Cytoplasm

A

Blue/Pink

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28
Q

Wright-Giemsa or Jenner-Giemsa Stain: Nuclei

A

purple/blue

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29
Q

Wright-Giemsa or Jenner-Giemsa Stain: cytoplasm

A

pink/blue

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30
Q

Wright-Giemsa or Jenner-Giemsa Stain: Eosinophils

A

Pink/Red

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31
Q

Peroxidase reaction for myeloid cells: M. cells (except basophils)

A

Green to Dark Blue Granules

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32
Q

Cytoplasmic Stains: Eosin Y, Eosin B, Phloxine B

A

Red

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33
Q

Cytoplasmic Stains: Picric Acid, Orange G, Rose Bengal

A

Yellow

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34
Q

Cytoplasmic Stains: Light Green SF, Lissamine Green

A

Green

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35
Q

Nuclear Stains: Neutral Red, Safranin O, Carmine, Hematoxylin

A

Red

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36
Q

Nuclear Stains: Methelyne Blue, Toluidine Blue, Celestine Blue

A

Blue

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37
Q

Periodic Acid Schiff: Pass Positive Subs

A

Red or Magenta Red

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38
Q

Periodic Acid Schiff: Nuclei

A

Blue

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39
Q

PAS with diastase for Glycogen: Glycogen

A

Red

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40
Q

PAS with diastase for Glycogen: Nuclei

A

Blue-Black

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41
Q

Best Carmine: Glycogen

A

Bright Red Granules

42
Q

Best Carmine: Nuclei

A

Blue or Grayish Blue

43
Q

Best Carmine: Mucin, Fibrin

44
Q

Langhan’s Iodine Method for Glycogen (Caleton’s Mod): Glycogen

A

Mahogany Brown

45
Q

Langhan’s Iodine Method for Glycogen (Caleton’s Mod): Tissue Constituents

46
Q

Fresh Frozen azure A metachromatic staining for glycosaminoglycans: glycosaminoglycans

A

Red-Purple

47
Q

Fresh Frozen azure A metachromatic staining for glycosaminoglycans: Tissue bg

48
Q

Alcian Blue Technique: Acid mucins

49
Q

Alcian Blue Technique: Nuclei

50
Q

Metachromatic Toluidine Blue Staining: Glycosaminoglycans

A

Red-Purple

51
Q

Metachromatic Toluidine Blue Staining: Tissue Bg

52
Q

Combined Aldehyde Fuchsin-Alcian Blue: Sulfated Mucin

53
Q

Combined Aldehyde Fuchsin-Alcian Blue: Carboxylated Mucins

54
Q

Mucicarmine Stain: Mucins

55
Q

Mucicarmine Stain: Nuclei

56
Q

Hale’s Dialyzed (colloidal) Iron Technique: Acid Mucin

57
Q

Hale’s Dialyzed (colloidal) Iron Technique: Nuclei

58
Q

Fluorescent Acridine Orange Technique: Acid mucopolysaccharides

59
Q

Fluorescent Acridine Orange Technique: Fungi

A

Greenish Red Fluorescence

60
Q

Fluorescent Acridine Orange Technique: Bg

A

Reddish Orange Flourescence

61
Q

Sudan IV (Scharfach R) for Lipids: Lipids (triglycerides)

62
Q

Sudan IV (Scharfach R) for Lipids: Nuclei

A

Blue/Black

63
Q

Oil red O method in dextrin: Fat

A

Brilliant Red

64
Q

Oil red O method in dextrin: Nuclei

65
Q

Osmic acid stain for fats: Nuclei

A

Yellow Orange

66
Q

Osmic acid stain for fats: Fats

67
Q

Nile Blue Sulfate for Fats: Neutral Fat

A

Pinkish Red

68
Q

Nile Blue Sulfate for Fats: Cholesterin esters and cholesterin fatty acids

69
Q

Nile Blue Sulfate for Fats: cerebrosides

A

light blue

70
Q

Nile Blue Sulfate for Fats: fatty acids and soap

A

deep blue to violet

71
Q

Toluidine blue - acetone method for sulfatide

A

Sulfatide deposits stain metachromatic red brown or yellow

72
Q

Borhydride-Periodic-Schiff (BHPS) method for glycosides: Gangliosides

73
Q

Borhydride-Periodic-Schiff (BHPS) method for glycosides: Nuclei

74
Q

Alkaline fast - green method for basic proteins: Histones and protamines (nuclei founded)

75
Q

Peracicitc Acid - alcian blue for cystine and cysteine

A

Blue Green

76
Q

Sakaguchi’s Test for Arginine

A

Orange-Red

77
Q

Gomori calcium method for ALP: ALP activity

A

brownish-black

78
Q

Gomori calcium method for ALP: Nuclei

79
Q

Gomori lead method for ACP: ACP activity

80
Q

Gomori lead method for ACP: Nuclei

81
Q

Lead method for 5-nucleotidase (Wachstein and Meisel: 5’ nucleotidase

A

Blackish-Brown Deposits

82
Q

a-naphtyl acetate method for nonspecific esterase: estrease activity

83
Q

a-naphtyl acetate method for nonspecific esterase: nuclei

84
Q

Indoxyl acetate method for nonspecific esterase (Holt and Withers): Esterase Activity

85
Q

Indoxyl acetate method for nonspecific esterase (Holt and Withers): Nuclei

86
Q

Tetrazolium method for monoamine oxidase (glenner et al) - monoamine oxidase activity

A

Bluish Black

87
Q

Feulgen Technique for Nuclear DNA: DNa

A

red-purple

88
Q

Feulgen Technique for Nuclear DNA: Cytoplasm

89
Q

Methyl Green - pyronin method for RNA and DNA: DNA (chromatin)

A

Green to Blue Green

90
Q

Methyl Green - pyronin method for RNA and DNA: RNA (nucleoli)

91
Q

Acridine Orange Fluorescent Staining for RNA and DNA: DNA

A

yellow-green fluorescence

92
Q

Acridine Orange Fluorescent Staining for RNA and DNA: RNA

A

brick to orange red

93
Q

Gomori’s silver impregnation stain for reticulin: Reticulin Fibers

94
Q

Van Gieson’s Stain for Collagen: Collagen

A

Pink or Deep Red

95
Q

Van Gieson’s Stain for Collagen: Muscle, Cytoplasm, RBC and Fibrin

96
Q

Van Gieson’s Stain for Collagen: Nuclei

A

Brownish Black to Black

97
Q

Masson’s Trichrome Stain: Muscle, RBC, Keratin

98
Q

Masson’s Trichrome Stain: Nuclei

A

Blue-Black

99
Q

Masson’s Trichrome Stain: Collagen, Mucus

100
Q

Weigert’s Elastic Tissue Stain: Elastic Fibers

A

Dark Blue or Blue-Black on Clear BG

mt (54 decks)

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