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STAINS IN HP - PART 1 Flashcards

1
Q

Highman’s Congo Red Method for Amyloid: Amyloid

A

Deep Pink to Red

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2
Q

Highman’s Congo Red Method for Amyloid: Nuclei

A

Blue

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3
Q

Alkaline Congo Red Technique: Amyloid, Elastic Fibers, Eosinophil Granules

A

Red

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4
Q

Krajian’s Amyloid Stain (mod. Bennhold method): Amyloid

A

Red on a clear background

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5
Q

Krajian’s Amyloid Stain (mod. Bennhold method): Nuclei

A

Blue

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6
Q

Methyl Violet - Crystal Violet Method: Amyloid

A

Purplish Red

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7
Q

Methyl Violet - Crystal Violet Method: Nuclei, Cytoplasm, CT

A

shade of violet

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8
Q

Mod. Gomori’s Trichome Stain: Muscle Fiber

A

Red

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9
Q

Mod. Gomori’s Trichome Stain: Collagen

A

Green

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10
Q

Mod. Gomori’s Trichome Stain: Nuclei

A

Blue to Black

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11
Q

Mallory’s PTAH: Muscle, Neuroglia

A

Blue

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12
Q

Mallory’s PTAH: Myelin

A

Blue to Blue-Gray

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13
Q

Mallory’s PTAH: Collagen

A

Reddish

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14
Q

Mallory’s PTAH: Coarse Elastic Fibers

A

Bluish

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15
Q

Mallory’s PTAH: Fibrin

A

Blue

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16
Q

Mallory’s PTAH: Nuclei

A

Deep Blue

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17
Q

Heidenhain’s Iron Hematoxylin: Muscle Striations, Mitochondria, Myelin and Chromatin

A

Grey to Black

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18
Q

Lissamin fast red - tartrazine method for muscles and bones: Muscles, Rbcs

A

Red

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19
Q

Lissamin fast red - tartrazine method for muscles and bones: Collagen

A

Yellow

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20
Q

Lissamin fast red - tartrazine method for muscles and bones: Nuclei

A

Black

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21
Q

Schmorl’s picro-thionin method: Lacunae, Canaliculi

A

Dark brown-black

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22
Q

Schmorl’s picro-thionin method: Bone Matrix

A

yellow or brownish yellow

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23
Q

Schmorl’s picro-thionin method: cells

A

Red

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24
Q

Rapid toluidine - eosin stain for glycol-methacrylate sections: Eosinophhilic granules

A

Red

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25
Rapid toluidine - eosin stain for glycol-methacrylate sections: Basophilic and Mast Cell Granules
Blue
26
Rapid toluidine - eosin stain for glycol-methacrylate sections: Nuclei
Blue
27
Rapid toluidine - eosin stain for glycol-methacrylate sections: Cytoplasm
Blue/Pink
28
Wright-Giemsa or Jenner-Giemsa Stain: Nuclei
purple/blue
29
Wright-Giemsa or Jenner-Giemsa Stain: cytoplasm
pink/blue
30
Wright-Giemsa or Jenner-Giemsa Stain: Eosinophils
Pink/Red
31
Peroxidase reaction for myeloid cells: M. cells (except basophils)
Green to Dark Blue Granules
32
Cytoplasmic Stains: Eosin Y, Eosin B, Phloxine B
Red
33
Cytoplasmic Stains: Picric Acid, Orange G, Rose Bengal
Yellow
34
Cytoplasmic Stains: Light Green SF, Lissamine Green
Green
35
Nuclear Stains: Neutral Red, Safranin O, Carmine, Hematoxylin
Red
36
Nuclear Stains: Methelyne Blue, Toluidine Blue, Celestine Blue
Blue
37
Periodic Acid Schiff: Pass Positive Subs
Red or Magenta Red
38
Periodic Acid Schiff: Nuclei
Blue
39
PAS with diastase for Glycogen: Glycogen
Red
40
PAS with diastase for Glycogen: Nuclei
Blue-Black
41
Best Carmine: Glycogen
Bright Red Granules
42
Best Carmine: Nuclei
Blue or Grayish Blue
43
Best Carmine: Mucin, Fibrin
Weak Red
44
Langhan's Iodine Method for Glycogen (Caleton's Mod): Glycogen
Mahogany Brown
45
Langhan's Iodine Method for Glycogen (Caleton's Mod): Tissue Constituents
Yellow
46
Fresh Frozen azure A metachromatic staining for glycosaminoglycans: glycosaminoglycans
Red-Purple
47
Fresh Frozen azure A metachromatic staining for glycosaminoglycans: Tissue bg
Blue
48
Alcian Blue Technique: Acid mucins
Blue
49
Alcian Blue Technique: Nuclei
Red
50
Metachromatic Toluidine Blue Staining: Glycosaminoglycans
Red-Purple
51
Metachromatic Toluidine Blue Staining: Tissue Bg
Blue
52
Combined Aldehyde Fuchsin-Alcian Blue: Sulfated Mucin
Blue
53
Combined Aldehyde Fuchsin-Alcian Blue: Carboxylated Mucins
Blue
54
Mucicarmine Stain: Mucins
Red
55
Mucicarmine Stain: Nuclei
Blue
56
Hale's Dialyzed (colloidal) Iron Technique: Acid Mucin
Dark Blue
57
Hale's Dialyzed (colloidal) Iron Technique: Nuclei
Red
58
Fluorescent Acridine Orange Technique: Acid mucopolysaccharides
Black
59
Fluorescent Acridine Orange Technique: Fungi
Greenish Red Fluorescence
60
Fluorescent Acridine Orange Technique: Bg
Reddish Orange Flourescence
61
Sudan IV (Scharfach R) for Lipids: Lipids (triglycerides)
Red
62
Sudan IV (Scharfach R) for Lipids: Nuclei
Blue/Black
63
Oil red O method in dextrin: Fat
Brilliant Red
64
Oil red O method in dextrin: Nuclei
Blue
65
Osmic acid stain for fats: Nuclei
Yellow Orange
66
Osmic acid stain for fats: Fats
Black
67
Nile Blue Sulfate for Fats: Neutral Fat
Pinkish Red
68
Nile Blue Sulfate for Fats: Cholesterin esters and cholesterin fatty acids
Light Red
69
Nile Blue Sulfate for Fats: cerebrosides
light blue
70
Nile Blue Sulfate for Fats: fatty acids and soap
deep blue to violet
71
Toluidine blue - acetone method for sulfatide
Sulfatide deposits stain metachromatic red brown or yellow
72
Borhydride-Periodic-Schiff (BHPS) method for glycosides: Gangliosides
Red
73
Borhydride-Periodic-Schiff (BHPS) method for glycosides: Nuclei
Blue
74
Alkaline fast - green method for basic proteins: Histones and protamines (nuclei founded)
Green
75
Peracicitc Acid - alcian blue for cystine and cysteine
Blue Green
76
Sakaguchi's Test for Arginine
Orange-Red
77
Gomori calcium method for ALP: ALP activity
brownish-black
78
Gomori calcium method for ALP: Nuclei
Green
79
Gomori lead method for ACP: ACP activity
Black
80
Gomori lead method for ACP: Nuclei
Green
81
Lead method for 5-nucleotidase (Wachstein and Meisel: 5' nucleotidase
Blackish-Brown Deposits
82
a-naphtyl acetate method for nonspecific esterase: estrease activity
blue
83
a-naphtyl acetate method for nonspecific esterase: nuclei
Green
84
Indoxyl acetate method for nonspecific esterase (Holt and Withers): Esterase Activity
Blue
85
Indoxyl acetate method for nonspecific esterase (Holt and Withers): Nuclei
Red
86
Tetrazolium method for monoamine oxidase (glenner et al) - monoamine oxidase activity
Bluish Black
87
Feulgen Technique for Nuclear DNA: DNa
red-purple
88
Feulgen Technique for Nuclear DNA: Cytoplasm
Green
89
Methyl Green - pyronin method for RNA and DNA: DNA (chromatin)
Green to Blue Green
90
Methyl Green - pyronin method for RNA and DNA: RNA (nucleoli)
Rose-Red
91
Acridine Orange Fluorescent Staining for RNA and DNA: DNA
yellow-green fluorescence
92
Acridine Orange Fluorescent Staining for RNA and DNA: RNA
brick to orange red
93
Gomori's silver impregnation stain for reticulin: Reticulin Fibers
black
94
Van Gieson's Stain for Collagen: Collagen
Pink or Deep Red
95
Van Gieson's Stain for Collagen: Muscle, Cytoplasm, RBC and Fibrin
Yellow
96
Van Gieson's Stain for Collagen: Nuclei
Brownish Black to Black
97
Masson's Trichrome Stain: Muscle, RBC, Keratin
Red
98
Masson's Trichrome Stain: Nuclei
Blue-Black
99
Masson's Trichrome Stain: Collagen, Mucus
Blue
100
Weigert's Elastic Tissue Stain: Elastic Fibers
Dark Blue or Blue-Black on Clear BG

mt (54 decks)

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