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mt > ADHESIVES, MOUNTING MEDIA AND MICROTOMY > Flashcards

ADHESIVES, MOUNTING MEDIA AND MICROTOMY Flashcards

1
Q

COMMON TYPES OF ADHESIVES

A

MAYER’S EGG ALBUMIN
DRIED ALBUMIN
GELATIN
GELATIN-FORMALDEHYDE MIXTURE
STARCH PASTE
PLASMA
POLY-L-LYSINE
APES (3-AMINOPROPYLTHRIETHOXYSILANE)

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2
Q

dried albumin components

A

5 grams albumin, 5 g NaCl, 100cc distllled water

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3
Q

gelatin (1%) components

A

1 gm gelatin, 100m water, 15ml glycerol, 2 gm penol crystals

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4
Q

Gelatin-formaldehyde mixture

A

1% gelatin 5ml, 2% formaldehyde 5ml

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5
Q

starch paste composition

A

1 g powdered starch, 30ml water, 2 drops HCl and thymol crystals.

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6
Q

plasma adhesive composition

A

Plasma is readily available from outdated blood stored in blood banks, dispensed into sterile tubes of 0.5ml each.

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7
Q

can be purchased as a 0.1% solution which is further diluted at 1:10 with distilled water (final dilution to 0.01%) prior to use. Sections are coated with this solution and allowed to dry.

A

Poly-L-Lysine

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8
Q

widely used as a section adhesive in immunohistochemistry.

A

Poly-L-Lysine

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9
Q

for cytospin preparations of proteinaceous or bloody material.

A

APES (3-Aminopropylthriethoxysilane)

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10
Q

Is usually syrupy fluid applied between the section and the coverslip after staining, setting the section firmly, preventing the movement of the coverslip.

A

mounting medium

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11
Q

CHARACTERISTICS OF GOOD MOUNTING MEDIUM

A

Avoid tissue distortion
Miscible with xylene and toluene
Should not dry quickly
Should not crack or produce artefactual granularity on the slide upon drying
Should not dissolve out or fade tissue sections
Should not cause shrinkage and tissue distortions
Should not leach out any stain or affect staining
Should not change color or pH
Should set hard, producing permanent mounting of sections

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12
Q

mounting medium 2 main groups

A
  1. aqueous media
  2. resinous mounting media
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13
Q

Are designed to mount water-miscible preparations directly from water in cases where the stain is removed or decolorized with alcohol or xylene

A

aqueous media

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14
Q

example of aqueous media

A

water, glycerin

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15
Q

Has low refractive index, moderately transparent and evaporates easily

A

water

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16
Q

Has high refractive index, provides greater visibility if slightly diluted with water.

A

Glycerin

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17
Q

Glycerin media example

A

Glycerin Jelly
Farrant’s Medium
Apathy’s Medium
Brun’s Fluid

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18
Q

Are used for preparations that have been dehydrated and cleared in xylene or toluene.

A

Resinous Mounting Media

19
Q

Transparent, almost colorless oleoresin that adheres firmly to glass and sets to a hard consistency without granulation

A

Canada Balsam

20
Q

canada balsam is dissolved in xylene in an incubator at ____ or paraffin oven at ___

A

37 deg.c ; 58 deg celsius

21
Q

Resinous medium, for small tissue sections

A

DPX - dibutyl phtalate and xylene (Refractive Index 1.532)

22
Q

refractive index 1.52
Synthetic resin mixture in xylene

A

XAM

23
Q

refractive index 1.544
synthetic resin soluble in xylene
preferred than DPX

A

clarite

24
Q

Is the process of sealing the margins of the cover slip to prevent the escape of fluid or semi-fluid mounts and evaporation of mountant
immobilized the coverslip
Prevent sticking of the slides upon storage

A

ringing

25
Q

For cutting serial sections of large blocks of paraffin embedded tissues

A

rocking microtome

26
Q

For cutting sections for electron microscopy

A

ultrathin microtome

27
Q

For cutting paraffin embedded sections

A

rotary microtome

28
Q

For cutting celloidin embedded sections

A

sliding microtome

29
Q

For cutting umembedded frozen sections

A

freezing microtome

30
Q

for manual sharpening when cutting edge has been rendered blunt or nicked

A

belgium yellow

31
Q

gives more polishing effect than the belgium yellow

A

arkansas

32
Q

much coarser than the first two types and is used only for badly nicked knives followed by either one of the first two knife sharpeners

A

fine carborundum

33
Q

hone is about ____ long; remove ____

A

8”x3” long; remove irregularities

34
Q

Its surface is wiped clean with a soft moistened with xylene in order to remove the scattered small particles of stones and metal.

Then, covered with a thin film of mineral and clove oil, xylene, liquid paraffin or soapy water for lubrication

A

hone

35
Q

direction of hone

A

Heel (handle end) to toe (head portion) direction

36
Q

hone knife

A

10-20x minot/plane wedge knife

37
Q

Used vibrating frosted glass plate or wheel driven by an electrical motor
Approximately 30 double strokes are given each side of the knife to which very gently pressure is applied
Quite expensive
Time saving
Produced well sharpened knives with uniform levels

A

MECHANICAL MICROTOME KNIFE SHARPENING

38
Q

precaution for mechanical microtome knife sharpening

A

lubricated with warm soapy water or fine oil before using, then washed with water
Presure should be gentle
Strokes should be 20-30x in each direction
After honing wipe off the oil or soap from the knife with xylene
Then strop it thoroughly

39
Q

BURR formed during honing is removed
To polish and sharpen the cutting edge
Toe to heel direction
Paddle strop made up of best quality horse leather
40-120 double strokes

A

Stropping (polishing)

40
Q

PRECAUTIONS IN STROPPING

A

Knife should be wiped clean with cloth
Knife edge is oiled or greased to prevent rusting
Strop should be treated with vegetable oil (castor oil) applied at the back of the strop not the surface; it should not be used for at least 24-48 hrs after treatment
Mineral oil is NOT recommended

41
Q

2 types of disposable blades

A

glass knives
diamond knives

42
Q

used fro trimming and semi-thin sectioning of tissue blocks for EM

A

glass knives

43
Q

cut any type of resin block for electron microscopy

A

diamond knives

44
Q

a disposable blades that is Brittle and expensive
Very durable

A

diamond knives

mt (54 decks)

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