NON-ENTERIC GI PATHOGENS Flashcards
Not a human microbiota, facultatively anaerobic and monotrichous organisms.
Found in brackish water, marine water, or salt water
Vibrio (Comma-shaped or Curved bacillus)
Vibrio (Comma-shaped or Curved bacillus) can be isolated from
algae, plankton, fish, and shellfish
vibrio are Halophilic organisms except for
V. cholera and V. mimicus
vibrio mode of acquisition
Consumption of raw or undercooked seafood
vibrio diseases and infections
Cholera, wound infection, septicaemia, and necrotizing faciitis
vibrio common isolates
V. cholerae O1 and non-O1, V. parahaemolyticus, V. vulnificus and V. alginolyticus
vibrio microscopy
Gram-negative, short, curved, asporogenous rods.
Gram-negative, short, curved bacilli (comma-shaped bacilli)
Facultative anaerobe
With monotrichous flagella
Vibrio
vibrio is catalase, oxidase and string test (_)
positive
vibrio grows on alkaline media such as
TCBS - Thiosulfate-citrate-bile salts-sucrose agar
vibrio are ___ fermenters
Glucose
Causative agent of cholera
Vibrio cholerae
Vibrio cholerae on TCBS
yellow colonies (sucrose fermenters)
Vibrio cholerae are non-halophilic (T or F)
True
common cause of cholera
Vibrio cholerae 01
an acute diarrheal infection that is mainly spread through contaminated water sources.
cholera
Vibrio cholerae virulence factor
choleragen
hallmark of cholera
Rice-watery stool (10-30 times of defecation per day)
protein toxin produced mainly by V. cholerae O1 strain
choleragen
responsible for the pandemic spread in v. cholerae
Somatic O1 & O139
3 major subgroups of V. cholerae (H & O antigen)
- V. cholerae O1
- V. cholerae O139
- V. cholerae non O1/non O139
V. cholerae O1 serotypes:
- Hikojima
- Ogawa
- Inaba
V. cholerae O1(pandemic type) Biotypes :
- Classical
- El Tor
in Polymyxin Susceptibility, classical is ___ and el tor is ____
classical = susceptible and el tor = resistant
in Lysis of bacteriphage, classical is ___ and el tor is ___
classical = negative and el tor = positive
in Chicken RBC agglutination classical is. ___ and el tor is ____
classical = negative and el tor = positive
in Hemolysis of sheep RBC, classical is ____ and el tor is ____
classical = negative and el tor = positive
in Vogues Proskauer, classical is ____ and el tor is ____
classical = negative and el tor = positive
Second most common Vibrio species that is associated with gastroenteritis
Vibrio parahaemolyticus
Vibrio parahaemolyticus was the etiologic agent of the outbreak of ____ in Japan in 1950
“summer diarrhea”
Vibrio parahaemolyticus selective medium
Wagatsuma agar ( high salt mannitol medium)
“Kanagawa phenomenon”
B-hemolysis
Vibrio parahaemolyticus in TCBS
green colonies (non-sucrose fermenters)
Vibrio parahaemolyticus are halophilic (T or F)
TRue