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AUBF PREGNANCY TEST Flashcards

1
Q

what are the 4 biological method in pt

A

frog’s test
ascheim-zondek test
friedmann’s test
hoffman’s test

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2
Q

Most reliable and accurate biological method of pregnancy testing

A

Frog’s Test

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3
Q

what type of frog is used in frog’s test

A

Green grass frog in rice field

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4
Q

Collection of urine specimen in frog’s test

A

anytime as long as the specimen is freshly voided

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5
Q

female’s frog test

A

hogben

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6
Q

principle of hogbens test

A

When Human Chorionic Gonadotropin (HCG) is injected to female frogs; it will cause extrusion of eggs/ova.

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7
Q

scientific name of the clawed foot frog or south african frog used in hogben

A

Xenopus laevis

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8
Q

in hogben’s ___ is produced

A

Egg Cell

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9
Q

preparation for female frog

A
  • 1 week old female frog
  • Frogs must stain from food for 12 hours
  • Extract frog’s urine at cloaca
    (vagina/frog part where to urinate
    “butt”)
  • Examine EGG CELL of frog.
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10
Q

male’s frog test

A

galli-mainini test

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11
Q

principle of galli-mainini

A

When Human Chorionic Gonadotropin (HCG) is injected to a male frog; it will cause the release of cigar- shaped spermatozoa.

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12
Q

scientific name of male frog

A

Rana viteggera (vittigera) or Rana pipiens

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13
Q

galli-mainini produces ____

A

sperm cell

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14
Q

purpose of frog test

A
  • Determine Pregnancy
  • Determine true pregnancy from other
    usual conditions
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15
Q

a condition of molar pregnancy, or also known as resemblance of “latu-latu”

A

Hydatidiform Mole

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16
Q

a condition of cancer of testes, “terra”

A

Terratoma

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17
Q

Quantitative test used to differentiate true pregnancy from chorioepithelioma.

A

Ascheim-Zondek Test

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18
Q

Principle of Ascheim-Zondek Test

A

When Human Chorionic Gonadotropin (HCG) is injected to immature female white mice; it will cause enlargement of ovary and appearance of hemorrhagic spots macroscopically and the ripening of graffian follicle of corpus luteum microscopically.

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19
Q

reddish color (macroscopically) in Ascheim

A

Corpura hemorrhagica

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20
Q

yellowish color (microscopically) in Ascheim

A

Corpura lutea

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21
Q

animal used in Ascheim

A

Immature Female white mice

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22
Q

weight of mice in ascheim zondek

A

5-8 grams

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23
Q

the age of mice in ascheim

A

21-31 days old

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24
Q

urine specimen in ascheim should be

A

24 hours

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25
in ascheim, the mice is.
killed 96 to 100 hours after 1st injection and After killing the mice, performed AUTOPSY using ETHER.
26
Quantitative test used in suspended hydatidiform, terratoma and tumor of testes.
Friedmann's Test
27
principle of friedmann's
When HCG is injected in female virgin rabbit it will produce 1-6 corpura hemorrhagica in the surface of ovary. The ovary become full and may also have corpura lutea.
28
Animal used in Friedmann
Female Virgin Rabbit
29
specimen in friedmann
urine w/c is injected in the marginal ear
30
resemble/same with friedmann
Hoffman's Test
31
specimen in hoffman
serum
32
what are the immunological pregnancy test
direct p.t indirect p.t
33
immunological pt where urine is mixed with anti-serum (same with blood typing)
Direct P.T
34
in direct pt, a ___ should be observed.
agglutination
35
reading of agglutination in direct pt
With Agglutination: POSITIVE Without Agglutination: NEGATIVE
36
test in direct pt
Dap Test
37
a test in direct pt that uses urine and serum and early as 4 days after, the result is already observe.
Dap Test
38
in indirect pregnancy agglutination is also observed where the results are read as..
✓ With Agglutination: NEGATIVE ㄨ Without Agglutination: POSITIVE
39
tests in indirect pt
Gravindex Ortho PT Denco PT
40
a slide test for pregnancy where no agglutination is positive
Gravindex
41
Sensitive as early as 42 days after the onset of the last menstruation.
Ortho PT
42
Ortho PT result is interpreted by..
appearance of turbidity where presence of turbidity = positive.
43
Denco PT is interpreted through..
- “doughnut-like” pattern is POSITIVE. - “MAT” pattern only (test tube) is NEGATIVE.
44
considered as less accurate and less popular.
Chemical PT
45
types of chemical pt
prenandiol test iodine or lugol's reaction test
46
a chemical pt that detect present of PROGESTERONE.
Prenandiol Test
47
the interpretation of results in Prenandiol
orange to deep brown is POSITIVE - amber & other colors are NEGATIVE.
48
chemical pt that Detects presence of the fight and flight emergency hormone EPINEPHRINE.
Iodine or Lugol's Reaction Test
49
interpretation of result in Iodine or Lugol's Reaction Test
deep orange to brown is POSITIVE.
50
A PT done by the clinicians/physicians.
Clinician's Test
51
types of clinician test
Prostigmen Withdrawal Bleeding Test Skin Colostrum Test Primodos Oral Test Gestex Tablet
52
process of Prostigmen Withdrawal Bleeding Test
- Prostigmen is injected to patients. - Prostigmen injection is used. - If woman menstruate 2 weeks after, she is not pregnant
53
skin colostrum test preparation
Colostrum preparation is injected to subcutaneous of arms of the patient. (COLOSTRUM – 1st injection of the mammary gland.)
54
interpretation of skin colostrum test
- If woman is not pregnant area will swell showing a REDDISH coloration and develop an allergy reaction. - If the woman is pregnant, the body would not react as it would only secrete colostrum substances. - RED and SWELLING is negative; NOT RED and NOT SWELLING is positive.
55
a clinician test that utilizes tablet/drugs
Primodos Oral Test Gestex Tablet
56
in Primodos Oral Test, a tablet is taken..
2 tablets taken once a day for 2 consecutive days. (4 pcs) 4pcs/day.
57
interpretation of result of Primodos Oral Test
If woman menstruate 2-3 days after, she is not pregnant.
58
a tablet mistakenly considered as abortion tablet but is not.
Gestex Tablet
59
in gestex tablet, a tablet is taken..
orally taken (1 tablet per day)
60
interpretation of result in gestex tablet
Within 4-5 days, the woman bleeds if she is not pregnant, no bleeding occurs if she is pregnant.
61
a pt that is no longer used in the present time in clinical laboratories as it may cause false-positive pregnancy test results.
Biological PT
62
purpose of PT
1) Confirmation of early normal pregnancy. 2) Evaluation of abnormal pregnancy. 3) Study of trophoblastic disease.
63
causes of animal's death in test
alkaline urine toxic urine susceptible animal bacterial contamination
64
when test animal dies due to alkaline urine, the remedy is to..
acidify the urine by adding 0.1N HCl (the pH should at least be 6.0)
65
when test animal dies due to toxic urine where the toxic substances causes the death of animal, the remedy is to
Detoxify with Kaolin
66
If the test animal is sick it is considered as
Susceptible Animal
67
when a sick (susceptible) animal is injected, it would most likely to die as they are prone to disease therefore if this happens, the research should..
Find and make use of another animal instead.
68
urine containing bacteria would most likely cause an infection to human. when the urine of those who are diagnosed with UTI/infections is used, the test animal would die. therefore, the remedy to this is to
Acidify the urine using 0.1N HCl
69
The HORMONE can be concentrated by adding
1. Kaolin 2. Absolute alcohol 3. Acetone
70
Defined as thespecific gonadotropic activity of 0.1 mg of HCG preparation which is required to produce cornification of vaginal epithelium of immature female rat.
International Unit
71
It is same with IU (measured as IU) but expressed in cc or smallest amount of urine that produces positive result.
Mouse Unit
72
produced in the placenta by the trophoblastic cells during the early months of pregnancy and is also measured as IU.
HCG - Human Chorionic Gonadotropic
73
CAUSES OF FALSE-POSITIVE PREGNANCY TEST
Ectopic Pregnancy Increase Follicle-stimulating Hormone (FSH) Appendicitis Abortion or Miscarriage Seasonal variation of test animals
74
a false-positive pt where Fetus is developed outside the uterus (most Fallopian tube)
Ectopic Preganancy
75
what hormone produced by Anterior Pituitary Gland when increased may cause a false-positive pt?
Follicle-stimulating Hormone
76
how does abortion or miscarriage cause a false-positive pt?
happens when there is an abortion or miscarriage happened and the mother did not perform Dilatation & Curettage (D&C) cleansing of the uterus.
77
CAUSES OF FALSE NEGATIVE PREGNANCY TEST
1. Low titer of concentration of hormone 2. Low sensitivity of animal 3. Toxic urine

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