ENTEROBACTERIACIAE - SALMONELLA INFECTION to Flashcards
One of the most common forms of food poisoning.
Gastroenteritis
Gastroenteritis is commonly caused by
S. enterica subsp. Enterica that comes from animals
The Salmonella serotype Typhimurium outbreak in the U.S. in 2009 came from __
contaminated peanut butter crackers.
Gastroenteritis sources of infection
Poultry products, milk, and handling of pets.
Gastroenteritis mode of dessimenation
Contaminated kitchen utensils
symptoms of gastroenteritis
Nausea, vomiting, fever and chills, watery diarrhea, and abdominal pain.
Febrile disease that develops from eating contaminated food prepared by infected individuals or carriers and direct transmission through fomites is also possible
Enteric fever ( Typhoid fever)
Enteric fever ( Typhoid fever) source of infection
Human carriers, contaminated food, and water
Enteric fever causes of outbreaks
Improper sewage disposal, poor sanitation, and lack of clean water source.
enteric fever symptoms
Malaise, anorexia, myalgia, and severe frontal headache
enteric fever complications
Necrosis in the gallbladder ( necrotizing cholecystitis) and Peyer’s patches
the hallmark of enteric fever infection
Appearance of “rose spots” during the second week of fever
The site of long term carriage for enteric fever
gallbladder
Occurs with or without extraintestinal infection that is caused by non-typhoidal Salmonella species.
Characterized by prolonged fever and intermittent bacteremia.
Bacteremia
Bacteremia causative agents
Salmonella serotype Typhimurium, Salmonella serotype Paratyphi, and Salmonella serotype Choleraesius.
The carriers of Salmonella excrete the organisms along with their feces and thus cause ___
“ accidental” contamination of food and water.
salmonella carriers can be treated by ___ if the gallbladder infection is not evident.
antimicrobial therapy
Isolation of Salmonella from culture plates is significant and specific for
diagnosis of thypoid fever
Specimens for Salmonella Identification
Blood – first week of infection
Stool – second to third week of infection
Urine – third week of infection
serological test for typhoid fever and detects for antibodies against the specific O and H antigens of S. typhi
Widal’s test-
Closely related to Escherichia.
Not member of the indigenous GI microbiota
Non-motile, intracellular pathogens that multiply within the cells of the intestinal epithelium
Shigella
disease of shigella and most species can cause bacillary dysentery
shigellosis
shigella reservoir
humans
shigella Modes of transmission
Flies, fingers, food, and feces ( four F’s) and water from infected persons (fecal-oral route)
shigella species
S.dysenteriae, S. flexneri, S. boydii and S. sonnei
shigella most virulent specie
S. dysenteriae
one of the causes of gay bowel syndrome
S. flexneri
shigella serogroups
A (S.dysenteriae), B (S.flexneri), C ( S. boydii), and D ( S. sonnei)
shigella antigenic structure
Somatic O
shigella culture
MAC – clear, fragile, and colorless (NLF)
SSA – colorless, without black centers
shigella IMViC reaction
v + - -
shigella TSIA reaction
K/A, (-) gas, (-) H2S
Shigella vs. E.coli
Shigella - NLF, Indole(-), non-motile
E. coli - LF, Indole(+), motile
Biochemical Characteristics of Shigella species:
- All species do not produce gas from glucose except for S. flexneri
- All species are mannitol fermenters except for S. dysenteriae.
- All species do not decarboxylate lysine
- All species do not decarboxylase ornithine, except for S. sonnei
- S. sonnei is a late lactose fermenter and has a positive reaction with the ONPG (O-nitrophenyl-beta-D-galactopyranoside)
All shigella species do not produce gas from glucose except for
S. flexneri
All shigella species are mannitol fermenters except for
S. dysenteriae
All shigella species do not decarboxylate lysine
YES THEY DO NOT HAHAHAH
All shigella species do not decarboxylase ornithine, except for
S. sonnei
shigella specie that is a late lactose fermenter and has a positive reaction with the ONPG (O-nitrophenyl-beta-D-galactopyranoside)
S. sonnei
Shigella dysenteriae is also known as
Shiga bacillus
Most virulent of the species and causes bacillary dysentery
Shigella dysenteriae (Shiga bacillus)
Shigella dysenteriae virulent factor
Shiga toxin
Shigella dysenteriae is urease
negative
Shigella dysenteriae IMViC reaction
v + - -