Prokaryotes Flashcards

1
Q

prokaryotes

A

domains bacteria and archea; about 3.5 bya; dominant; .5-5 micrometers; pervasive

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2
Q

chemical evolution hypothesis

A

heterotroph to photosynthetic autotroph to aerobes

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3
Q

cell wall

A

protection; prevents cell from bursting in a hypotonic environment; does not prevent plasmolysis in hypertonic environment; maintains shape of cell

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4
Q

cocci

A

balls/sphere; diplococcus (“2”); streptococcus (“chain”); staphylococcus (“clump”)

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5
Q

spirals

A

spirilium - “rigid”; spirochete - “flexible

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6
Q

bacilli

A

rods

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7
Q

peptidoglycan

A

only found in bacterial cell wall; polymer structure (sugars cross linked by short polypeptides); NOT in eu cell walls

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8
Q

plant cell wall

A

cellulose

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9
Q

fungi cell wall

A

chitin

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10
Q

gram staining

A

divides bacteria into two categories; 1st stain is “crystal violet” (purple); 2nd stain is “safernin” (pink);

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11
Q

gram positive

A

retains crystal violet (purple colored cell); cell wall w/ thick layer of peptidoglycan

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12
Q

penicillin

A

interfers w/ peptidoglycan synthesis and destroying gram positive bacteria

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13
Q

gram negative

A

cell wall w/ thin layer of peptidoglycan; doesn’t retain crystal violet; pink colored cells; outer membrane evolved to have LPS - toxic, induces fever

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14
Q

capsules and slime layers

A

surround cell wall, composed of polysaccharides and proteins; protect against phagocytosis

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15
Q

fimbrae and philli

A

hair - live appendages; fimbrae - shorter and more numerous; both used for attachment

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16
Q

endospores

A

dormant stage; w/stand harsh conditions; ex: anthrax

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17
Q

taxis

A

directed movement in response to stimuli

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18
Q

positive taxis

A

towards stimulus (ex: chemotaxis)

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19
Q

negative taxis

A

away from stimulus (ex: chemotaxis)

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20
Q

flagella

A

common motility found in bacteria, archaea, eu

21
Q

flagella: pro vs. eu

A

in pro 1/10 as wide and not covered by PM; diff propulsions; diff molecular compositions

22
Q

flagella: bac vs. archea vs. eu

A

similar functions; arose independently (analogous structure)

23
Q

flagella: bac vs. archae

A

similar size; similar propulsion mechanism; diff compositions

24
Q

prokaryotic flagellum

A

H pos gradient as H pos is pumped across PM; H pos diffuses through motors, produces force, turns hook, turns filament, taxis

25
3 parts of flagella
motor - rings embedded in cell wall and PM; rotating filament - propels cells; curved hook
26
prokaryotes lack
membrane bound organelles; such as nucleus, mitochondria, chloroplast
27
nucleoid region
in cytoplasm; location of chromo is in DNA (single chromosome)
28
plasmids
smaller sings of DNA, replicate independently; few nonessential genes
29
plasma membrane
extensively folded (greater surface area: greater volume); embedded enzymes for photosynthesis and cellular respiration
30
cytoplasm
ribosomes; storage granules; enzymes
31
basic reproduction
binary fission; optimal conditions - divides every 1-3 hours
32
rapid reproduction and mutations
error occur which cause genetic diversity via mutations; probability of mutation is 1 in 10 million per cell division
33
rapid evolution
mutations are rare per gene but genetic variability increases quickly in large pops w/ short gen times
34
genetic recombination
combining DNA from 2 different sources; meiosis and fertilization in eu; transformation, transduction, and conjugation in pro
35
horizontal gene transfer
movement of genes from one ind of one sp to ind of another sp; in prokaryotes
36
transformation
pro cels take up DNA from surroundings; Griffith Exp
37
transduction
phages transfer pro genes from HC to another; during phage rep frag of host DNA accidentally packages into new viral particles
38
conjugation
gen material transfered btwn pro cells; donor cell attaches to recipient via pillus, pulls it closer, transfers DNA (one way)
39
F-factor (fertility)
pice of DNA needed to make pillus; on bac chromo or on plasmid
40
F positive
cells w/ f-plasmid are DNA donors
41
F negative
cells w/out F-factor are DNA recipients
42
phototrophs
light E
43
chemotrophs
chemical E
44
autotrophs
get carbon from CO2
45
heterotrophs
organic nutrients (glucose)
46
obligate aerobes
requires oxygen
47
facultative anaerobes
can adapt to conditions
48
obligate anaerobes
nitrate ions and sulfate ions act as electron acceptors instead of O2
49
nitrogen metabolism
N cycle; N-fixation: bacteria turns N2 into NH3; nitrification: bacteria turns Nit3 into NO3-