(M) Nematoda: Morphology of Secernentea [Strongylida, Rhabtida, Oxyurida, Spirurida] (lecture-based) Flashcards
Strongylida, Rhabtida, Oxyurida, Spirurida
Order of the Subclass Secernentea
- Ascaridida
- Strongylida
- Rhabditida
- Oxyurida
- Spirurida
Species under Ancylostomatoidea
- Necator americanus
- Ancylostoma duodenale
- Ancylostoma caninum
- Ancylostoma braziliense
Order of the super family Ancylostomatoidea
Strongylida
Species under Metastrongyloidea
Angiostrongylus cantonensis
Strongylida superfamily
Species under Rhabdiascoidea
Strongyloides stercoralis
Order and Super family of Strongyloides stercoralis
Rhabditida & Rhabdiascoidea
Sp. under Oxyuroidea
Enterobius vermicularis
Sp. under Gnathostomatoidea
Gnathostoma spinigerum
Sp. under Filarioidea
- Wuchereria bancrofti
- Brugia malayi
- Loa loa
- Onchocerca volvulus
- Mansonella ozzardi
- Mansonella persians
Strongylida
Feeding Stage
250 um
Open Mouth
Bulbous Esophagus
Long, Narrow Buccal Cavity
Rhabditiform larva
Strongylida
700 um
Closed Mouth
Pointed Tail
Filariform larvae
infective stage for hookrowms
idenfity larva
- 250 um; Short Buccal Cavity
- Prominent Primordial Genitalia
- Compared to hookworms, the primordial genitalia is more prominent in Strongyloides stercoralis.
- Club-Shaped Esophagus w/ Posterior
Rhabditiform larva
Rhabditida
DF Rhabditiform larva
Club-shaped esophagus
Oxyurida
Prominent Cephalic Alae.
Bulb-like Esophagus.
Female: Sharply-Pointed End
Male: Curved Caudal End
Egg: Described as D-Shaped or Lopsided Egg.
E. vermicularis
Oxyurida
DF of Male E. vermicularis
bulb-like esophagus
Spirurida
Has Sub-globose Cephalic swelling separated from the remainder of the body by Cervical Constriction.
Male: Pseudo-Bursa
Female: Slightly Post-equatorial in position
Gnathostoma spinigerum
Spirurida
Ovoid, Transparent Shell
Superficially Pitted
* Mucoid Plug in one end
Gnathostoma spinigerum
DF of Egg Gnathostoma spinigerum
Mucoid plug in one end
Strongylida
Male: 7-9 mm x 0.3 mm diameter
Female: 9-11 mm x 0.4 mm diameter
Egg: 64-76 x 36-40 microns elongate with transparent shell, 2-8 cell stage of development.
Filariform Larva: With conspicuous striations of the sheath, tail-end forms a wide angle with the sheath
NEcator americanus
Strongylida
Male: 8-11 mm x 0.4-0.5 mm diameter
Female: 10-13 mm x 0.6 mm diameter
Egg: Same as N. americanus but more rounded.
Filariform Larva: With less conspicuous striations of the sheath, narrow angle between tail and sheath.
A. duodenale
Metastrongyloidea
Adult Female
* Barber-pole Appearance.
* The Anterior End of Worm is on the Top.
* The Scale Bar is 1mm
Angiostrongylus cantonensis
Metastrongyloidea
- Tail of adult male of Angiostrongylus cantonensis, showing Copulatory Bursa and Long Spicules (arrows).
- Scale bar is 85 um.
Angiostrongylus cantonensis
Sheathed Filiaria worms
- Wuchereria bancrofti
- Loa loa
- Brugia malayi
Filarial worms
- The Adult Worms of W. bancrofti, inhabit the Lymphatic Vessels and Glands.
- Minute, Thread-Like, Smooth-Skinned, and Tapered on Both Ends.
- But Bluntly Rounded At The Most Terminal
Adult morphology W. bancrofti
Filarial worms
Size: 40mm long x 0.1mm in diameter
Sharply Curved Posterior.
Male W. bancrofti
Filarial worms
Size: 80-100mm x 0.24-0.30mm
With Vulva at Cervical Region, Single Uterus, but all the remaining reproductive organs are paired.
Female W. bancrofti
Filarial worms
Size: 244-296um long x 7.5-10 um in diameter
Sheathed with Graceful Curves.
Bluntly Rounded Anterior w/ Short Space Devoid of Nuclei.
Pointed Caudal Part.
Smooth Cuticle.
Body Nuclei Are Equidistant And Regularly
Microfilaria Wuchereria bancrofti
Filarial worms
Male Size: 13-23 mm long x 70 - 80 um in breadth.
Female Size: 43 - 55mm long, 130 - 170 um.
Microfilaria Size: 177-230 um
Bluntly Rounded Anterior.
Sheathed, with a Stiff & Kinky Body.
Unevenly Distributed Body Nuclei.
Two Prominent Terminal Nuclei
Brugia malayi
Filarial worms
DF of Microfilaria size
2 prominent terminal nuclei
Filarial worms
Male Size: 30 - 34 mm x 0.35 - 0.43 mm
Female Size: 40 - 70 mm x 0.5 mm
Microfilaria Size: 250 - 300x6 - 8um
Rounded Anterior End
Very Small or No Cephalic Spaces
Irregular Curves of Body covered with Thin
Transparent Sheath.
Irregularly Distributed Body Nuclei that reached up to the Caudal End.
Loa loa
Filarial worms
Unsheathed filaria worms
- Onchocerca volvulus
- Mansonella ozzardi
- Mansonella perstans
Filarial worms
Found in in Lymphatic Fluids and Both Adults.
* May be found in the Skin and Subcutaneous
Tissues where they produce Fibrous Nodules.
Microfilariae O. volvulus
Filarial worms
this are are White, Opalescent, and Transparent.
* With Distinct Transverse Striations Of The
Adult worms O. volvulus
Filarial worms
Male Size: 19-24 mm long x 130-210 um.
Female Size: 3.5-50 mm x 270-400 um.
* Microfilariae Size: 315-360 um x 6 um.
* With Both Ends Free of Nuclei.
They may be found in the blood, urine, and sputum.
* But more commonly in the Lymphatic of the
Connective Tissues.
* Cutaneous layers in an Area around the Adult
Worms in the Corneal Conjunctivae.
O. volvulus
Filarial worms
Male Size: 45 mm long × 60 um
Female Size: 70 - 80 mm long × 120 um
Microfilaria Size: 200 um x 4.5
* The body nuclei fill up the entire body.
* No Distinct Space at Cephalic or Tail
Mansonella perstans
NECATOR AMERICANUS (New World Hookworm)
DF of Rhabditiform larva
Inconspicuous genital primordium at midpoint
NECATOR AMERICANUS (New World Hookworm)
DF of the copulatory bursa
Bi-partite dorsal ray (characteristic feature)
NECATOR AMERICANUS (New World Hookworm)
DF Necator americanus buccal sample
buccal capsule with a pair of thin semi-lunar cutting plates
ANCYLOSTOMA DUODENALE (Old World Hookworm)
DF Rhabditiform larva
Inconspicuous genital primordium at midpoint
ANCYLOSTOMA DUODENALE (Old World Hookworm)
DF filariform larva
W/ less conspicuous striations of the sheath, narrow angle between tail and sheath
ANCYLOSTOMA DUODENALE (Old World Hookworm)
Characteristic feature of the copulatory bursa
Tri-partite dorsal ray
Angiostrongylus cantonensis (Rodent Lung Worm)
Diagnostic feature of Adult female
“barber’s pole appearance”
STRONGYLOIDES STERCORALIS (THREADWORM)
DF of the Esophagus (rhabditiform larvae)
- Club-shaped Esophagus w/ Posterior Bulb
- Conspicuous/prominent genital primordium
STRONGYLOIDES STERCORALIS (THREADWORM)
DF of filariform larva
→ Notched & bifid caudal end
ENTEROBIUS VERMICULARIS (PINWORM, SEAT WORM, OR SOCIETY WORM)
DF of Male
bulb-like esophagus without true buccal capsule
ENTEROBIUS VERMICULARIS (PINWORM, SEAT WORM, OR SOCIETY WORM)
Egg shape
D-shaped or Lopsided egg appearance
Gnathostoma spinigerum
DF of adult
Cephalic end: sub-globose, the
swollen part w/ constriction
Gnathostoma spinigerum
DF of egg
Prominent mucus plug at 1 pole (diagnostic)
Spiral dark-colored body producing “barber’s pole” appearance
Angiostrongylus cantonensis
ENTEROBIUS VERMICULARIS
DF Gross/Adult
Cephalic alae and prominent bulbous esophagus