Lecture 1 Intro To Anatomy Flashcards
Anatomical position definition
Standing squarely on all 4 limbs with head and tail extended
Dorsal
Toward the back
Ventral
Toward the belly
Rostral aka
Cranial
Rostral
Closer to head along main body axis
Caudal
Closer to tail along main body axis
The hindlimb is ________ to the forelimb
Caudal
Thorax is _______ to the pelvis
Rostral
Which plane divides the animal into equal right and left halves
Median
Is the median plane unique
Yes, only have 1
Ipsilateral
On the same side of median plane
Contralateral
On opposite side in median plane
Medial
Towards midline (or median plane)
Lateral
Away from midline
How do we describe medial/lateral in limbs
Refer to inner and outer surfaces of limb
Sagittal plane
Parallel to median plane
How many sagittal planes are there
Infinite
Dorsal plane
Define dorsal vs ventral
How are limbs described in dorsal plane
-Dorsal = front of paws
-Palmar/plantar = rear/underside of forepaw and hindpaw
Transverse plane definition
Longitudinal plane perpendicular to median and dorsal planes
Transverse plane defines ________ vs ___________
Cranial vs caudal
How are limbs described in transverse plane
-proximal region is closer to trunk
-paw is most distal
-cranial and caudal refer to front and rear surfaces proximal to carpus and tarsus
Superficial
Towards surface of body or surface of solid organ
Deep
Away from surface of body
Skin is __________ to cutaneous trunci muscle
Superficial
Vertebral column is _________ to skin and back muscles
Deep
4 basic tissues
-epithelium
-connective tissue
-muscle
-nervous tissue
2 types of microscopy
-light microscopy
-electron microscopy
2 types of electron microscopy
-transmission electron microscopy
-scanning electron microscopy
How Light microscopy works
-photons interact with tissue components
-light is focused with optical lens
-histological stains
How electron microscopy works
-electrons interact with tissue components
-electrons focused with electromagnetic lens
What does TEM show
Internal structure
Areas that are electron dense in TEM appear
-dark because not many electrons have passed through
Areas that are electron lucent in TEM appear
Lighter grey color because more electrons have passed through
What does SEM show
External or surface structures
4 steps to prepare stained slides
-fixation
-embedding
-sectioning
-staining
Fixation
Stop biological processes
Embedding
Stabilize
Sectioning
Slice
2 basic types of stains/dyes
-basophilic
-acidophilic
Basophilic stain definition and appearance
-interact with acidic structures
-acidic components appear blue or purple
Example of basophilic stain
Hematoxylin
Acidophilic stain definition and appearance
-interact with basic structures
-turn components pink, red or orange
Example of acidophilic stain
Eosin
What is the most common stain used
-H & E (combination of hematoxylin and eosin)
Which dye is used to stain collagen fibers blue or green
Trichome
Which stain is used to stain carbohydrates magenta or purple
Periodic acid shift (PAS)
Which stain is used to stain elastic fibers reddish brown or black
Orcein
Artifacts: general definition
Something that is artificially made by people
Artifacts: histological definition
-Tissue shrinkage and lost molecules
-cracks and spaces in tissue under microscope