Lab 4: Protozoas

Question 1

Answer 1

giardia

Question 2

Answer 2

giardia

Question 3

— are slightly larger than a red blood cell and contain 2 or 4 nuclei, — and median bodies

Answer 3

Giardia
axostyles

Question 4

Answer 4

giardia cyst

Question 5

Answer 5

giardia trophozoite

Question 6

where do trophozoites of giardia usually live

Answer 6

attached to the intestinal mucosa

Question 7

giardia causes —

Answer 7

watery diarrhea

Question 8

giardia trophozoites look

Answer 8

pear shaped
x2 size of a red blood cell
2 nuclei, median body and axostyle
free flagellae and sucking disk

Question 9

Answer 9

cryptosporidium parvum oocysts

acid fast +

Question 10

yeast is what color in acid fast stain

Answer 10

blue or green because they are acid fast negative

acid fast + are pink

Question 11

cat

Answer 11

cystoisospora felis
cystoisospora rivolta

Question 12

— in dogs are identical to C. rivolta in cats

Answer 12

Cystoisospora ohioensis

small 20 mcm (twice the size of giardia)

Question 13

— found in cats and dogs are twice the size of giardia

Answer 13

cat: cystoisospora rivolta oocysts

dog: cystoisospora ohioensis

Question 14

— found in cat and dog are 3 times the size of giardia

Answer 14

Cystoisospora felis
Cystoisospora canis

(half the size of a whipworm egg- trichuris- lemon shape)

Question 15

— occysts sporulate 48 hr after being passed

Answer 15

Toxoplasma gondii

cat
same size as giardia

identical to Neospora caninum in dogs

Question 16

Neospora caninum is the same size as —

Answer 16

Toxoplasma gondii in cats

or

giardia 10 mcm

Question 17

cat

Answer 17

Toxoplasma gondii

identical to Neospora caninum in dogs

Question 18

Answer 18

toxoplasma gondii
Neospora caninum

Question 19

goat

Answer 19

eimeria

thin wall at one pole of the oocyst (micropyle)

also found in birds, pigs and herbivores

Question 20

what is the thinning at one pole of the oocyst of Eimeria

Answer 20

micropyle

Question 21

dog

Answer 21

sarcocystis

sporulated in the feces

Question 22

Answer 22

Question 23

Answer 23

Babesia canis

Question 24

pear shape

Answer 24

pyriform

piriform- babesia, theleria, Cytauxzoon

Question 25

Babesia canis is found

Answer 25

red blood cell of dogs

Question 26

Answer 26

nucleus and kinetoplast amastigote- Leishmania donovani

Question 27

amastigotes

Answer 27

protist cell that does not have visible external flagella or cilia. intercellular phase of replication for Leishmania donovani

Question 28

unicellular animals that are classified on the basis of the organelles used for locomotion (flagella, pseudopodia, cilia or no observable organelle

Answer 28

protozoa

Question 29

--- move is a leaf like fashion

Answer 29

Giardia

Question 30

Giardia normally forms cysts, but when there is extreme diarrhea the --- may be washed out of the small intestine and quickly pass through the colon.

Answer 30

trophozoites pear shaped

Question 31

These are much quicker moving than Giardia, but you should be able to see the undulating membrane as a flickering wave on the body of the trophozoite as it rolls over while moving forward

Answer 31

Tritrichomonas trophozoites

Question 32

--- don’t form cysts, so the diagnosis depends on identifying the trophozoite

Answer 32

Tritrichomonas spp.

Question 33

where does Tritrichomonas spp. live in cattle

Answer 33

reproductive tract of the cow and on the penis of the bull

Question 34

--- cause cow abortion and have undulating membrane

Answer 34

Tritrichomonas foetus

Question 35

--- cause chronic cat diarrhea

Answer 35

Tritrichomonas blagburni

Question 36

To detect the Giardia cysts, what process would you use

Answer 36

zinc centrifuge float with drop of iodine added

Question 37

for giardia, The --- stage is usually found in the feces of infected animals, but in diarrheic stools the --- can sometimes be seen

Answer 37

cyst trophozoites

Question 38

to see trophozoites of giardia what test

Answer 38

liquid stool make a direct smear and look for leaf like movement use zinc to see cysts

Question 39

what do giardia cysts look like

Answer 39

small 10 mcm oval shape remnants of the trophozoite organelles

Question 40

Answer 40

Cystoisospora Sarcocystis Toxoplasma

Question 41

Answer 41

eimeria

Question 42

Answer 42

crypto

Question 43

Answer 43

Question 44

--- cause autoinfection

Answer 44

cryptosporidium

Question 45

what test to show crypto

Answer 45

fecal float and acid fast staining (stains red)

Question 46

Toxoplasma gondii looks that same as

Answer 46

Neospora caninum in dogs Hammondia spp. in dogs and some cats

Question 47

how to tell neospora from hammondia and toxoplasma apart

Answer 47

neospora and toxoplasma have serology test IgM + = + for disease IgG= old disease

Question 48

Oocysts of T. gondii are shed for--- during the first infection (usually in young cats) and the cat is then immune to re-infection.

Answer 48

only 1 or 2 weeks immune compromised older cats might shed

Question 49

how long to sporulate Toxo cyst

Answer 49

48-72 hours

Question 50

Parasites of this genus occur in carnivores and omnivores and are very host species specific. not usually pathogenic and live on epithelial cells of small intestine

Answer 50

cystoisospora

Question 51

cystoisospora --- measures about 40 μm long while C. --- will measure about 20 μm

Answer 51

canis, felis ohioensis, rivolta

Question 52

are passed unsporulated and take 24 to 48 hours to sporulate and reach infectivit

Answer 52

cystoisospora

Question 53

Parasites of this genus occur in herbivores, swine, birds and omnivores and are host species specific. Oocysts range from 11 to 80 μm long.

Answer 53

Eimeria

Question 54

Some of the oocysts of Eimeria will have a structure known as a --- (a thinning of the oocyst wall

Answer 54

micropyle

Question 55

Parasites of this genus occur in carnivores (definitive hosts) and herbivores (intermediate hosts).

Answer 55

Sarcocystis

Question 56

sarcocystis sporocyst in feces look

Answer 56

sporulates in the DH

Question 57

These parasites live within red blood cells and are very small.

Answer 57

Babesia

Question 58

what test to find babesia

Answer 58

The infected cells tend to be “sticky” and will accumulate within capillaries. Thus, it is more likely to detect an infection if you make a blood smear from a skin prick, then from blood drawn from a vein. The infected cells tend to be larger than normal RBC and thus will accumulate with the WBC when blood is spun, thus a smear of the buffy coat will be more likely to yield a positive result

Question 59

common parasites in tropical and sub-tropical areas of the world that are transmitted by --- flies

Answer 59

Leishmania donovani phlebotomine sand flies

Question 60

what type of dogs common for Leishmania donovani

Answer 60

fox hounds

Question 61

Leishmania have --- inside them

Answer 61

kinetoplast