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Fall 22: Parasites > Lab 4: Protozoas > Flashcards

Lab 4: Protozoas Flashcards

1
Q
A

giardia

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2
Q
A

giardia

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3
Q

— are slightly larger than a red blood cell and contain 2 or 4 nuclei, — and median bodies

A

Giardia
axostyles

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4
Q
A

giardia cyst

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5
Q
A

giardia trophozoite

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6
Q

where do trophozoites of giardia usually live

A

attached to the intestinal mucosa

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7
Q

giardia causes —

A

watery diarrhea

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8
Q

giardia trophozoites look

A

pear shaped
x2 size of a red blood cell
2 nuclei, median body and axostyle
free flagellae and sucking disk

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9
Q
A

cryptosporidium parvum oocysts

acid fast +

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10
Q

yeast is what color in acid fast stain

A

blue or green because they are acid fast negative

acid fast + are pink

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11
Q

cat

A

cystoisospora felis
cystoisospora rivolta

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12
Q

— in dogs are identical to C. rivolta in cats

A

Cystoisospora ohioensis

small 20 mcm (twice the size of giardia)

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13
Q

— found in cats and dogs are twice the size of giardia

A

cat: cystoisospora rivolta oocysts

dog: cystoisospora ohioensis

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14
Q

— found in cat and dog are 3 times the size of giardia

A

Cystoisospora felis
Cystoisospora canis

(half the size of a whipworm egg- trichuris- lemon shape)

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15
Q

— occysts sporulate 48 hr after being passed

A

Toxoplasma gondii

cat
same size as giardia

identical to Neospora caninum in dogs

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16
Q

Neospora caninum is the same size as —

A

Toxoplasma gondii in cats

or

giardia 10 mcm

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17
Q

cat

A

Toxoplasma gondii

identical to Neospora caninum in dogs

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18
Q
A

toxoplasma gondii
Neospora caninum

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19
Q

goat

A

eimeria

thin wall at one pole of the oocyst (micropyle)

also found in birds, pigs and herbivores

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20
Q

what is the thinning at one pole of the oocyst of Eimeria

A

micropyle

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21
Q

dog

A

sarcocystis

sporulated in the feces

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22
Q
A
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23
Q
A

Babesia canis

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24
Q

pear shape

A

pyriform

piriform- babesia, theleria, Cytauxzoon

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25
Q

Babesia canis is found

A

red blood cell of dogs

26
Q
A

nucleus and kinetoplast

amastigote- Leishmania donovani

27
Q

amastigotes

A

protist cell that does not have visible external flagella or cilia.

intercellular phase of replication for Leishmania donovani

28
Q

unicellular animals that are classified on the basis of the organelles used for locomotion (flagella, pseudopodia, cilia or no observable organelle

A

protozoa

29
Q

— move is a leaf like fashion

A

Giardia

30
Q

Giardia normally forms cysts, but when there is extreme diarrhea the — may be washed out of the small intestine and quickly pass through the colon.

A

trophozoites

pear shaped

31
Q

These are much quicker moving than Giardia, but you should be able to see the undulating membrane as a flickering wave on the body of the trophozoite as it rolls over while moving forward

A

Tritrichomonas trophozoites

32
Q

— don’t form cysts, so the diagnosis depends on identifying the trophozoite

A

Tritrichomonas spp.

33
Q

where does Tritrichomonas spp. live in cattle

A

reproductive tract of the cow and on the penis of the bull

34
Q

— cause cow abortion and have undulating membrane

A

Tritrichomonas foetus

35
Q

— cause chronic cat diarrhea

A

Tritrichomonas blagburni

36
Q

To detect the Giardia cysts, what process would you use

A

zinc centrifuge float

with drop of iodine added

37
Q

for giardia, The — stage is usually found in the feces of infected animals, but in diarrheic stools the — can sometimes be seen

A

cyst
trophozoites

38
Q

to see trophozoites of giardia what test

A

liquid stool make a direct smear and look for leaf like movement

use zinc to see cysts

39
Q

what do giardia cysts look like

A

small 10 mcm
oval shape
remnants of the trophozoite organelles

40
Q
A

Cystoisospora
Sarcocystis
Toxoplasma

41
Q
A

eimeria

42
Q
A

crypto

43
Q
A
44
Q

— cause autoinfection

A

cryptosporidium

45
Q

what test to show crypto

A

fecal float and acid fast staining (stains red)

46
Q

Toxoplasma gondii looks that same as

A

Neospora caninum in dogs
Hammondia spp. in dogs and some cats

47
Q

how to tell neospora from hammondia and toxoplasma apart

A

neospora and toxoplasma have serology test
IgM + = + for disease
IgG= old disease

48
Q

Oocysts of T. gondii are shed for— during the first infection (usually in young cats) and the cat is then immune to re-infection.

A

only 1 or 2 weeks

immune compromised older cats might shed

49
Q

how long to sporulate Toxo cyst

A

48-72 hours

50
Q

Parasites of this genus occur in carnivores and omnivores and are very host species specific. not usually pathogenic and live on epithelial cells of small intestine

A

cystoisospora

51
Q

cystoisospora — measures about 40 μm long while C. — will measure about 20 μm

A

canis, felis

ohioensis, rivolta

52
Q

are passed unsporulated and take 24 to 48 hours to sporulate and reach infectivit

A

cystoisospora

53
Q

Parasites of this genus occur in herbivores, swine, birds and omnivores and are host species specific. Oocysts range from 11 to 80 μm long.

A

Eimeria

54
Q

Some of the oocysts of Eimeria will have a structure known as a — (a thinning of the oocyst wall

A

micropyle

55
Q

Parasites of this genus occur in carnivores (definitive hosts) and herbivores (intermediate hosts).

A

Sarcocystis

56
Q

sarcocystis sporocyst in feces look

A

sporulates in the DH

57
Q

These parasites live within red blood cells and are very small.

A

Babesia

58
Q

what test to find babesia

A

The infected cells tend to be “sticky” and will accumulate within capillaries. Thus, it is more likely to detect an infection if you make a blood smear from a skin prick, then from blood drawn from a vein. The infected cells tend to be larger than normal RBC and thus will accumulate with the WBC when blood is spun, thus a smear of the buffy coat will be more likely to yield a positive result

59
Q

common parasites in tropical and sub-tropical areas of the world that are transmitted by — flies

A

Leishmania donovani

phlebotomine sand flies

60
Q

what type of dogs common for Leishmania donovani

A

fox hounds

61
Q

Leishmania have — inside them

A

kinetoplast

Fall 22: Parasites (32 decks)

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