Lab 4: Protozoas Flashcards

1
Q
A

giardia

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2
Q
A

giardia

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3
Q

— are slightly larger than a red blood cell and contain 2 or 4 nuclei, — and median bodies

A

Giardia
axostyles

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4
Q
A

giardia cyst

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5
Q
A

giardia trophozoite

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6
Q

where do trophozoites of giardia usually live

A

attached to the intestinal mucosa

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7
Q

giardia causes —

A

watery diarrhea

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8
Q

giardia trophozoites look

A

pear shaped
x2 size of a red blood cell
2 nuclei, median body and axostyle
free flagellae and sucking disk

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9
Q
A

cryptosporidium parvum oocysts

acid fast +

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10
Q

yeast is what color in acid fast stain

A

blue or green because they are acid fast negative

acid fast + are pink

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11
Q

cat

A

cystoisospora felis
cystoisospora rivolta

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12
Q

— in dogs are identical to C. rivolta in cats

A

Cystoisospora ohioensis

small 20 mcm (twice the size of giardia)

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13
Q

— found in cats and dogs are twice the size of giardia

A

cat: cystoisospora rivolta oocysts

dog: cystoisospora ohioensis

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14
Q

— found in cat and dog are 3 times the size of giardia

A

Cystoisospora felis
Cystoisospora canis

(half the size of a whipworm egg- trichuris- lemon shape)

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15
Q

— occysts sporulate 48 hr after being passed

A

Toxoplasma gondii

cat
same size as giardia

identical to Neospora caninum in dogs

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16
Q

Neospora caninum is the same size as —

A

Toxoplasma gondii in cats

or

giardia 10 mcm

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17
Q

cat

A

Toxoplasma gondii

identical to Neospora caninum in dogs

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18
Q
A

toxoplasma gondii
Neospora caninum

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19
Q

goat

A

eimeria

thin wall at one pole of the oocyst (micropyle)

also found in birds, pigs and herbivores

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20
Q

what is the thinning at one pole of the oocyst of Eimeria

A

micropyle

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21
Q

dog

A

sarcocystis

sporulated in the feces

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22
Q
A
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23
Q
A

Babesia canis

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24
Q

pear shape

A

pyriform

piriform- babesia, theleria, Cytauxzoon

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25
Babesia canis is found
red blood cell of dogs
26
nucleus and kinetoplast amastigote- Leishmania donovani
27
amastigotes
protist cell that does not have visible external flagella or cilia. intercellular phase of replication for Leishmania donovani
28
unicellular animals that are classified on the basis of the organelles used for locomotion (flagella, pseudopodia, cilia or no observable organelle
protozoa
29
--- move is a leaf like fashion
Giardia
30
Giardia normally forms cysts, but when there is extreme diarrhea the --- may be washed out of the small intestine and quickly pass through the colon.
trophozoites pear shaped
31
These are much quicker moving than Giardia, but you should be able to see the undulating membrane as a flickering wave on the body of the trophozoite as it rolls over while moving forward
Tritrichomonas trophozoites
32
--- don’t form cysts, so the diagnosis depends on identifying the trophozoite
Tritrichomonas spp.
33
where does Tritrichomonas spp. live in cattle
reproductive tract of the cow and on the penis of the bull
34
--- cause cow abortion and have undulating membrane
Tritrichomonas foetus
35
--- cause chronic cat diarrhea
Tritrichomonas blagburni
36
To detect the Giardia cysts, what process would you use
zinc centrifuge float with drop of iodine added
37
for giardia, The --- stage is usually found in the feces of infected animals, but in diarrheic stools the --- can sometimes be seen
cyst trophozoites
38
to see trophozoites of giardia what test
liquid stool make a direct smear and look for leaf like movement use zinc to see cysts
39
what do giardia cysts look like
small 10 mcm oval shape remnants of the trophozoite organelles
40
Cystoisospora Sarcocystis Toxoplasma
41
eimeria
42
crypto
43
44
--- cause autoinfection
cryptosporidium
45
what test to show crypto
fecal float and acid fast staining (stains red)
46
Toxoplasma gondii looks that same as
Neospora caninum in dogs Hammondia spp. in dogs and some cats
47
how to tell neospora from hammondia and toxoplasma apart
neospora and toxoplasma have serology test IgM + = + for disease IgG= old disease
48
Oocysts of T. gondii are shed for--- during the first infection (usually in young cats) and the cat is then immune to re-infection.
only 1 or 2 weeks immune compromised older cats might shed
49
how long to sporulate Toxo cyst
48-72 hours
50
Parasites of this genus occur in carnivores and omnivores and are very host species specific. not usually pathogenic and live on epithelial cells of small intestine
cystoisospora
51
cystoisospora --- measures about 40 μm long while C. --- will measure about 20 μm
canis, felis ohioensis, rivolta
52
are passed unsporulated and take 24 to 48 hours to sporulate and reach infectivit
cystoisospora
53
Parasites of this genus occur in herbivores, swine, birds and omnivores and are host species specific. Oocysts range from 11 to 80 μm long.
Eimeria
54
Some of the oocysts of Eimeria will have a structure known as a --- (a thinning of the oocyst wall
micropyle
55
Parasites of this genus occur in carnivores (definitive hosts) and herbivores (intermediate hosts).
Sarcocystis
56
sarcocystis sporocyst in feces look
sporulates in the DH
57
These parasites live within red blood cells and are very small.
Babesia
58
what test to find babesia
The infected cells tend to be “sticky” and will accumulate within capillaries. Thus, it is more likely to detect an infection if you make a blood smear from a skin prick, then from blood drawn from a vein. The infected cells tend to be larger than normal RBC and thus will accumulate with the WBC when blood is spun, thus a smear of the buffy coat will be more likely to yield a positive result
59
common parasites in tropical and sub-tropical areas of the world that are transmitted by --- flies
Leishmania donovani phlebotomine sand flies
60
what type of dogs common for Leishmania donovani
fox hounds
61
Leishmania have --- inside them
kinetoplast