Gene Expression Flashcards

1
Q

Central dogma

A

DNA coding to proteins

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2
Q

What does RNA have

A

Uracil (GCAU), ribose sugar and some are single stranded

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3
Q

You take the sense to template strand from

A

5 to 3

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4
Q

Transcription steps

A

RNA polymerase opens up DNA, read template strand which requires Ribnucleoside triphosphate

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5
Q

rRNA

A

Ribosomal RNA

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6
Q

MiRNA

A

Protein coding genes

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7
Q

What are promoter regions

A

Specific sequence allows binding of RNA polymerase

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8
Q

Transcription factors bind to

A

TATA and recruit RNA onto promoter

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9
Q

What can activate transcription

A

Phosphorylation

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10
Q

What does RNA splicing do

A

Remove introns and glue Exons together

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11
Q

Start and stop codons are both

A

Exons

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12
Q

What defines introns

A

Uniques sequence

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13
Q

Generation of a lariot is

A

Axons put together

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14
Q

What generate variance of a gene

A

Splicing

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15
Q

In translation if the reading frame shifts it causes

A

Different proteins

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16
Q

What do used to read mRNA

A

TRNA

17
Q

What reads mRNA codon

A

Anticodon loop

18
Q

The ribosome has 2

A

Subunits one large and one small which is made from proteins and RRNA

19
Q

Steps to control gene expression

A

Stop making mRNA
Stop splicing process
Stop transport of mRNA

20
Q

MiRNA

A

Non coding RNAs
50% are processed from introns
Short

21
Q

MiRNA cause

A

Transcriptional repression by degrading mRNA targets, as they bind to target mRNA complimentary to break down, this helps control gene expression

22
Q

Loss of MiRNA causes

A

Loss of transcriptional control and cancer

23
Q

What acts as a switch for transcription

A

Phosphorylation of RNA polymerase

24
Q

MRNA is read in groups of

A

Three bases or codons which make up the reading frame

25
Q

Each codon encodes a particular

A

Amino acid