Food Testing RP (P1)

Question 1

Test for starch

Answer 1

Add 3ml of test solution to a test tube
Add 5 drops of iodine solution

Positive black/purple
Negative orange

Question 2

Simple sugars

Answer 2

Add 5ml of your solution to a test tube
Add 10 drops of Benedicts solution
Place it in a 70-80 degree water bath for 5 minutes

Positive-brick red
Negative- blue

Question 3

Lipids

Answer 3

Add 5ml of your test solution to a test tube
Add 3 drops of Sudan III
Gently shake

Positive-red layer at surface
Negative-no change

Question 4

Protein

Answer 4

Add 2ml of your test solution to a test tube
Add 2 ml of biuret solution
Gently shake

Positive-purple
Negative-blue

Question 5

Where is amylase produced?

Answer 5

Salivary glands
Pancreas
Small intestine

Question 6

Where is lipase produced?

Answer 6

Salivary glands
Pancreas
Small intestine
Stomach

Question 7

Where is protease produced

Answer 7

Pancreas

Small intestine

Question 8

Sample preparation method

Answer 8

Use a pestle and mortar to grind down a small sample of food
Transfer this to a small beaker and add distilled water
Stir in order to allow some of the food to dissolve
Use a filter tunnel and paper to remove excess food
Half-fill a test tube with this solution

Question 9

Lipids preferred method

Answer 9

5ml solution
3 drops ethanol
gently shake

Positive: cloudy white
Negative: colourless

Question 10

Protein option 2

Answer 10

2ml solution
1ml sodium hydroxide
1ml copper sulfate
Gently shake

Positive: purple
Negative: colourless