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Clinical Chemistry > Clinical Enzymology Pancreatic mark > Flashcards

Clinical Enzymology Pancreatic mark Flashcards

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1
Q

Classification of pancreatic enzymes

A

Hydrolase

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2
Q

Smallest pancreatic enzyme

A

Amylase

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3
Q

Function of amylase

A

Breaks down carbohydrates: starch & glycogen

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4
Q

Coenzymes for amylase

A

Calcium and Chloride

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5
Q

Diagnostic significance of amylase

A

Acute pancreatitis, mumps, parotitis, macroamylasemia

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6
Q

Time profile of amylase in acute pancreatitis

A

Increases in 5-8 hours, peaks at 24 hours, normalizes in 3-5 days

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7
Q

Isoenzymes of amylase

A

Ptyalin and Amylopsin

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8
Q

Source and characteristics of Ptyalin

A

Salivary amylase (1,2,3); present in fallopian tubes and lungs; predominant in serum; faster migration in electrophoresis

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9
Q

Source and characteristics of Amylopsin

A

Pancreatic amylase (1,2,3); predominant in urine

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10
Q

Predominant amylopsin isoenzyme in acute pancreatitis

A

P3

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11
Q

Chemical inhibitor of salivary amylase

A

Wheat germ

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12
Q

Specificity of lipase

A

High specificity, sustained elevation due to increased half-life

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13
Q

Coenzymes for lipase

A

Bile salts and Colipase

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14
Q

Time profile of lipase in acute pancreatitis

A

Increases in 4-8 hours, peaks at 24 hours, normalizes in 7-14 days

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15
Q

Primary source of lipase

A

Pancreas

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16
Q

Specimen considerations for amylase determination

A

Avoid intake of pain relievers and use of lipemic serum

17
Q

Spectrophotometric method: Measures disappearance of starch substrate; decrease in color of starch-iodine complex

A

Amyloclastic

18
Q

Spectrophotometric method: Measures appearance of product; increases reducing sugars as starch is hydrolyzed by AMS; classic reference method

A

Saccharogenic

19
Q

Spectrophotometric method: Measures increasing color from production of product coupled with a chromogenic dye

A

Chromogenic

20
Q

UV method: Coupled-enzymatic- coupling of several enzyme systems; monitors amylase activity by measuring increased Abs of NADH at

A

340 nm

21
Q

Reference values for serum amylase

A

25–130 U/L

22
Q

Reference values for urine amylase

A

1–15 U/h

23
Q

Specimen considerations for lipase determination

A

Avoid hemolysis

24
Q

Cherry-Crandall method for lipase substrate

A

olive oil

25
Q

Product of Cherry-Crandall method”.”glycerol + 3 fatty acids

A
26
Q

Method that measures rate of clearing as fats are hydrolyzed by LPS

A

Turbidimetric

27
Q

Reference range for lipase

A

<38 U/L

28
Q

Rise time of amylase

A

5-8 hours

29
Q

Rise time of lipase

A

4-8 hours

30
Q

Peak time of amylase and lipase

A

24 hours

31
Q

Decline time of amylase

A

3-5 days

32
Q

Decline time of lipase

A

8-14 days

Clinical Chemistry (35 decks)

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