Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Clinical Chemistry > Clinical Enzymology Pancreatic mark > Flashcards

Clinical Enzymology Pancreatic mark Flashcards

1
Q

Classification of pancreatic enzymes

A

Hydrolase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Smallest pancreatic enzyme

A

Amylase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Function of amylase

A

Breaks down carbohydrates: starch & glycogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Coenzymes for amylase

A

Calcium and Chloride

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Diagnostic significance of amylase

A

Acute pancreatitis, mumps, parotitis, macroamylasemia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Time profile of amylase in acute pancreatitis

A

Increases in 5-8 hours, peaks at 24 hours, normalizes in 3-5 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Isoenzymes of amylase

A

Ptyalin and Amylopsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Source and characteristics of Ptyalin

A

Salivary amylase (1,2,3); present in fallopian tubes and lungs; predominant in serum; faster migration in electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Source and characteristics of Amylopsin

A

Pancreatic amylase (1,2,3); predominant in urine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Predominant amylopsin isoenzyme in acute pancreatitis

A

P3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Chemical inhibitor of salivary amylase

A

Wheat germ

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Specificity of lipase

A

High specificity, sustained elevation due to increased half-life

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Coenzymes for lipase

A

Bile salts and Colipase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Time profile of lipase in acute pancreatitis

A

Increases in 4-8 hours, peaks at 24 hours, normalizes in 7-14 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Primary source of lipase

A

Pancreas

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Specimen considerations for amylase determination

A

Avoid intake of pain relievers and use of lipemic serum

17
Q

Spectrophotometric method: Measures disappearance of starch substrate; decrease in color of starch-iodine complex

A

Amyloclastic

18
Q

Spectrophotometric method: Measures appearance of product; increases reducing sugars as starch is hydrolyzed by AMS; classic reference method

A

Saccharogenic

19
Q

Spectrophotometric method: Measures increasing color from production of product coupled with a chromogenic dye

A

Chromogenic

20
Q

UV method: Coupled-enzymatic- coupling of several enzyme systems; monitors amylase activity by measuring increased Abs of NADH at

21
Q

Reference values for serum amylase

A

25–130 U/L

22
Q

Reference values for urine amylase

A

1–15 U/h

23
Q

Specimen considerations for lipase determination

A

Avoid hemolysis

24
Q

Cherry-Crandall method for lipase substrate

25
Q

Product of Cherry-Crandall method”.”glycerol + 3 fatty acids

26
Q

Method that measures rate of clearing as fats are hydrolyzed by LPS

A

Turbidimetric

27
Q

Reference range for lipase

28
Q

Rise time of amylase

29
Q

Rise time of lipase

30
Q

Peak time of amylase and lipase

31
Q

Decline time of amylase

32
Q

Decline time of lipase

Clinical Chemistry (43 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions