Ch.1- Cell and Molecular Bio Flashcards
Generic Formula of Sugar
C(x)H(2x)Ox
Molecular Formula of Glucose
C6H12O6
Alpha-Glucose
OH on bottom (See notes)
Beta-Glucose
OH on top (See notes)
Carbons in Ribose
5
Bond in Dissacharides
Glycosidic linkage
Formed via condensation/dehydration reactions
Sucrose
Glucose + Fructose
Lactose
Glucose+ Galactose
Maltose
Glucose + Glucose
Starch
Energy storage in plant cells
Polymer of alpha- glucose molecules
Glycogen
Energy storage in animals
Polymer of alpha-glucose molecules
Has branhces
Cellulose
Structural role of walls in plant cells
Polymer of beta glucose
Beta-1,4 glycosidic - very rigid
Chitin
Structural role in walls of fungus and in exoskeletons of arthropods
Beta glucose polymer
Has beta 1,4 glycosidic bond
Has a N attached to each glucose molecule
Function of lipids
Insulation
Energy storage
Structural roles (cholesterol, phospholipids)
Endocrine function
Glycolipid
Similar to phospholipid
Contains CHO molecule instead of phosphate group
Steroids
Backbone form 4 C rings linked together
Prokaryotes don’t have sterols
Generic structure of Amino Acid
C bonded to
- Aminoa group
- Carboxyl group
- Hydrogen
- R group
Formation of peptide
Produces water
Forms Peptide bond using peptidyl transferases
Storage proteins
Casein (milk)
Ovalbumin (egg whites)
Transport proteins
Vesicles that transport materials in and out of cells
Cytochromes
Transport proteins that carry electrons
Defensive Proteins
Ab
Enzymes
Regulates rate of chemical reactions
Denaturation
Structures above secondary level is removed
Not broken down into individual amino acids
Usually irreversible, but can sometimes be reverse with the removal of denaturing agent
Nucleotides
N base
5 C sugar (i.e. deoxyribose)
Phosphate gorup
Neucleoside
Only sugar and base
Purines
A,G
2 rings
Pyrimadines
1 ring
C,T
DNA vs RNA
RNA has ribose not deoxyribose
U not T
Single stranded
Cell Theory
All living organisms are composed of 1+ cells
Cells are the basic unit of structure,function and organization in all organisms
All cells come from pre-existing, living cells
Cells carry hereditary information
RNA World Hypothesis
Self replicating RNA molecules were precursors to current life (based on DNA, RNA, proteins)
RNA stores genetic info like DNA, catalyzes chemical reactions
RNA unstable compared to DNA,, more likely to participate in chem reactions due to its extra OH group
Central Dogma of Genetics
Info in genes flows into proteins
DNA—> RNA—> Protein
Can’t go from protein back to nucleic acid
Ribozymes
RNA molecule capable of acting as an enzyme
Sometimes DNA is converted to RNA to function as an ribozyme
H bond Between C:G
3
H Bonds Between A:T
2
Amylose
Linear plant starch
Forms alpha 1, 4 glycosidic bonds
Amylopeptic
Branched from of plant starch
Alpha 1,4 (linear) or
Alpha 1,6-glycosidic (branches)
3 Structural Classifications of Protein
Fibrous, globular, intermediate
Fibrous Proteins
Insoluble
Long polymer fibers/sheets
Form structural components of cells
i.e collagen
Globular Proteins
Soluble
Tightly folded
i.e. Albumin
Intermediate Structural Proteins
Soluble
Fiber shaped
i.e. Fibrinogen
Conjugated Protein
Have aa and non-protein components
i.e. glycoproteins
Holoenzymes
Enzymes bound to their cofactor
Apoenzyme
Enzyme not bound to its cofactor
Bonds in TAG
Ester
Light Microscopes are good for seeing
Cell organization
Cell division
Movement
Electron Microscopy
Electrons have smaller wavelength= higher resolution and magnification
SEM
Focused beam of electrons scans sample’s surface
Good for studying surface topography, morphology, composition
TEM
Electrons transmitted through surface
Sample needs to be fixed first
Good for studying internal structures
Fluorescence Microscope
Good for studying movement in cell, across PM, or in protein interactions