Transcription and translation Flashcards
what happens first
Transcription
what is transcription
DNA is transferred and mRNA molecule is produced
translation
mRNA is translated and an amino acid sequence is produced
where does transcription happen
in nucleus
role of transcription
to produce a strand of mRNA
5 steps of transcription
1- parts of DNA molecule unwind when hydrogen bonds between base pairs break
2- this exposes the template strand of the gene that codes for protein being synthesised
3- free mRNA nucleotides present in nucleus bind to complementary nucleotides in template strand
4- the mRNA nucleotides join neighbouring nucleotides to form a single strand of mRNA
5- the mRNA molecule leaves nucleus vi a pore
3 differences between DNA and RNA
- DNA is larger / longer
- DNA is double stranded
- DNA contains thymine while RNA contains uracil
translation happens in
cytoplasm, in the ribosome
translation results in the production of
chain of amino acids
5 steps of Translation
1- after leaving nucleus, the mRNA attaches to a ribosome
2- in the cytoplasm, there are free tRNA molecules which have a triplet of unpaired bases at one end known as anticodons, and an amino acid at the other
3- the anticodon on each tRNA molecule pairs with a complementary triplet (codon) on the mRNA molecule, bringing its specific amino acid with it
4- a second tRNA molecule attaches to its complementary donor on the mRNA & a peptide bond is formed between the 2 naighbouring amino acids
5- This is continued until a stop codon on the mRNA is reached which signals the end of translation and means amino acid chain coded by mRNA is complete
the amino acid chain at the end of translation is _____ to form a protein
folded and modified