Tests for Fungal Infections

Question 1

for certain bacteria & fungi that possess the enzyme Urease w/c hydrolases Urea releasing ammonia into the medium.

Answer 1

Urease test

Question 2

This produces a change in the pH of the of the medium.

Answer 2

Urease test

Question 3

Effect of urea to fungi

Answer 3

increases microbial antagonism w/c reduces its survival

Question 4

Nitrate is transported from the medium into the cell cytosol where it is reduced to ammonium in 2 successive reactions catalyzed respectively by nitrate & nitrite reductase.

Answer 4

Nitrate assimilation in yeast

Question 5

an agar that is made up & inoculated w/ a yeast & a carbohydrate. It is incubated from 1 - 3 days & even up to 24 days to allow the identification of yeast that are slow metabolizers.

Answer 5

Assimilation test for fungi:

Question 6

is a polysaccharide predominant & specific constituent of fungal cell wall. It can be detected in serum during invasive fungal infections serving as a biomarker for Aspergillosis & invasive Candidiasis.

Answer 6

1 - 3 B - d - glucan

Question 7

may be employed for the maintenance of stock culture of fungi, especially the black pigmented varieties. Addition of glucose will enhance the chromogenesis of Trichophyton rubrum.

Answer 7

Cornmeal Agar

Question 8

is recommended for use in the cultivation of fungi & for the inducement of Chlamydospore formation by Candida albicans.

Answer 8

Tween 80 in Cornmeal agar

Question 9

reduces surface tension & enhances the development of Chlamydospores.

Answer 9

Polysorbate 80

Question 10

increases the level of a. acids, lipids, & organic acids in fungal cells. Helps to initiate alcoholic fermentation.

Answer 10

Thiamine

Question 11

combines magnetic resonance w/ nanotechnology to identify whole Candida spp. cells.

Answer 11

T2 Magnetic Resonance assay

Question 12

serves as enzymatic agent that breaks down debris in a specimen such as epithelial cells & WBC to view yeast or pseudohyphae. Freshly prepared is required because ___ can react w/ Carbon dioxide, giving wrong result.

Answer 12

KOH prep

Question 13

can irritate the lungs when inhaled.

Answer 13

Hazards of KOH

Question 14

with 2% glucose to support fungal growth & 0.5 ug/ml Methylene blue w/c enhances the zone edge definition.

Answer 14

Mueller - Hinton Agar for susceptibility testing