SYPHILIS AND RICKETSSIA Flashcards
: anti-phagocytic, mimic proteins on the heart valve
M Proteins
– to remove cross-reactivity
(Reiter strain)
ТРНА:
Turkey erythrocytes
Lyme disease Vector:
Ixodes scapularis tick
ANTISTREPTOLYSIN O TEST (ASTO/ASO)
If a patient’s serum containing [?] Ab is added to the streptolysin Rgt, an Ag-Ab reaction occurs causing the neutralization of the toxin
antistreptolysin O
Prepare
14 tubes
is the RBC control (no hemolysis) = (+) control
Tube 13
is the SLO control (hemolysis) = (-) control
Tube 14
Normal: Titer of
166 Todd units or below (0-166)
ANTI-DNASE B TESTING
Positive result: green complex (Intact DNAmethyl green conjugate because [?])
no hydrolysis
Murine typhus
R. typhi
Trench fever
Rartonella (Rochalimaea) quintana
Does not react with Weil Felix
Trench fever
Q fever
: OX-19 and OX-2
Proteus vulgaris
Capsular Antigen
Associated with virulent strains
Indicated carrier state
Vi Antigen
TYPHIDOT Aka
Dot Enzyme Immunoassay
• Most reliable indicator
Agglutination method
MYCOPLASMA PNEUMONIAE
Causes [?]/walking pneumonia
PAP (Primary atypical pneumonia)
not a serologic test but microbiolog ic assay
Culture
Look for growth on specialized agars
Culture
Difficult to grow and takes several weeks
Culture
Antibodies crossreact with I antigen on group O red cells; look for agglutination
Cold agglutinin testing
Easy to do but not specific for mycoplasma
Cold agglutinin testing
Patient ingests radioactive urea; bicarbonate produced, which is breathed out as CO2
Urea breath test
Rapid and sensitive but involves radioactivity
Urea breath test
o Indicates current or past infection and in most cases immunity
IgG antibodies to the virus
Acute Rheumatic fever Virulent factor:
M protein
WEIL-FELIX TEST Principle:
Direct Agglutination
SEROLOGICAL TESTS FOR SALMONELLA
Gold Standard:
Bacterial culture
MYCOPLASMA PNEUMONIAE Immunologic feature:
Cold agglutinins
BRUCELLA
: pink
Gram (-) bacteria
Line in IgG:
previously successfully treated
R. akari
Rickettsial pox