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IMMUNE 1 LEC > CHAPTER 6.1 - TYPES OF FIA, RIA, EIA > Flashcards

CHAPTER 6.1 - TYPES OF FIA, RIA, EIA Flashcards

1
Q
  1. Direct Immunofluorescent Assays

Reagent/s
Unknown

A

Antibody (labelled)

Antigen

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2
Q
  1. Indirect Immunofluorescent Assay
    Reagent/s
    Unknown
A

1) Solid-phase Antigen 2) Labelled antiimmunoglobulin

Antibody (unlabelled)

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3
Q
  1. Inhibition Immunofluorescence Test
    Reagent/s
    Unknown
A

1) Antigen 2) Antibody (Fluorescent Labelled)

Antibody

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4
Q
  1. Complement staining IF Test
    Reagent/s
    Unknown
A

1) Antibody 2) Compelment

1) Antigen 2) Fluorescent Labelled AntiCompelment

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5
Q

(+) Result Fluorescence

(+) Result NO Fluorescence

A
  1. Direct Immunofluorescent Assays
  2. Complement staining IF Test
  3. Fluorescence Polarization Immunoassay
  4. Inhibition Immunofluorescence Test
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6
Q
  1. Classic RIA Competitive Analysis
    Reagent/s
    Unknown
A

1) Antigen (unlabelled)

1) Solid-phase Antigen 2) Antigen (Radioisotope Labelled)

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7
Q
  1. Non-competitive Assays
    Reagent/s
    Unknown
A

1) Antigen

1) Solid-phase Antibody 2) Antibody (Radioisotope Labelled)

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8
Q
  1. Indirect Radioimmunosorbent assay
    Reagent/s
    Unknown
A

1) Anti-IgE (Solid Phase – coated in microtiter plate) 2) IgE (Radioisotope Labelled)

Patient IgE

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9
Q
  1. Direct Noncompetitive Binding Radioimmunosorbet Test
    Reagent/s
    Unknown
A

1) Anti-IgE (Solid Phase – coated in microtiter plate) 2) IgE (Radioisotope Labelled)

Patient IgE

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10
Q
  1. Radioprecipitation Test 6. Radioallergosorbent test
    Reagent/s
    Unknown
A

1) rabbit anti-human IgE 2) IgE (Radioisotope Labelled)

Patient IgE

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11
Q
  1. Competitive Enzyme-Linked Immunosorbent Assays (ELISA)
    Reagent/s
    Unknown
A

1) Solid Phase Antibody 2) Antigen (Enzymes Labelled)

Antigen (Unlabelled)

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12
Q
  1. Non-competitive Assays
    Unknown
A

Antibody

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13
Q
  1. Immunoenzymometric Test
    Reagent/s
    Unknown
A

1) Antibody (Enzymes Labelled) 2) Solid Phase Antigen

Antigen

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14
Q

(+) Result ↑ Radioactivity = ↓ Antigen

A
  1. Classic RIA Competitive Analysis
  2. Indirect Radioimmunosorbent assay
  3. Radioprecipitation Test
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15
Q

(+) Result ↑ Radioactivity = ↑ Antigen

A
  1. Radioallergosorbent test
  2. Direct Noncompetitive Binding Radioimmunosorbet Test
  3. Non-competitive Assays
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16
Q

(+) Result ↑ Enzyme Acivity (change in color) = ↓ Antigen

A
  1. Competitive Enzyme-Linked Immunosorbent Assays (ELISA)
17
Q

(+) Result ↑ Enzyme Acivity (change in color) = ↑ Antibody

A
  1. Non-competitive Assays

IMMUNE 1 LEC (28 decks)

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