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IMMUNE 1 LEC > DENGUE > Flashcards

DENGUE Flashcards

1
Q

DENGUE Family (same as Hep C)

A

Flaviviridae

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2
Q

DENGUE Structure

A

Envelope RNA virus

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3
Q

DENGUE Serotypes

A

4 sero types (DENV-1,2,3,4)

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4
Q

DENGUE Geographic distribution

A

Tropical and sub tropical countries

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5
Q

DENGUE Vector

A

Aedes aegypti

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6
Q

DENGUE MOT

A

Via vector

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7
Q

EPSTEIN-BARR VIRUS (EBV) Family

A

Herpesviridae

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8
Q

EPSTEIN-BARR VIRUS (EBV) Structure

A

Double stranded DNA

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9
Q

EPSTEIN-BARR VIRUS (EBV) MOT

A

Saliva, blood transfusion, transplacental

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10
Q

Gold standard for dengue

A

Viral Isolation

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11
Q

• 6-10 days to replicate viral cell culture

A

Viral Isolation

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12
Q

• Molecular detection of dengue genomic RNA; Testing of ribonucleic acid

A

RT-PCR

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13
Q

• NS1 is a highly conserved glycoprotein for virus viability

A

Dengue NS1

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14
Q

• Detects acute phase infection

A

Dengue NS1

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15
Q

First to become (+) prior to IgM as long as infected

A

Dengue NS1

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16
Q

• First detectable IgM only appears 4- 5 days

A

IgM-capture ELISA (MACELISA)

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17
Q

• Diagnosis of acute infection

A

IgM-capture ELISA (MACELISA)

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18
Q

IgM-capture ELISA (MACELISA) Presence of line in IgG only indicates

A

chronic infection or recovery

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19
Q

• SD BIOLINE Dengue Duo

A

Rapid test combo kit

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20
Q

• Detects Dengue NS1 Ag and IgG/IgM in a single test

A

Rapid test combo kit

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21
Q

: High viral load but declines at day 5

A

Acute Illness

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22
Q

• As the viral load decreases, the antibodies [?]

A

increase

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23
Q

• First increase [?]

A

IgM

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24
Q

→ past dengue infection

A

• IgG persists

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25
Q

→ already high in acute illness

A

• NS1 Ag

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26
Q

DIAGNOSTIC MARKERS FOR DENGUE
Acute illness:[?]
6 to 14 days: [?]

A

NS1

IgM followed by IgG

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27
Q

EBV DISEASES

A
  1. Infectious mononucleosis (IM)
  2. Other disease
    • Burkitt’s lymphoma
    • Nasopharyngeal CA
    • Neoplasms of thymuns, parotid gland (salivary gland) and larynx
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28
Q

• Kissing disease

A

Infectious mononucleosis (IM)

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29
Q

• Young adults affects the mononuclear phagocyte system

A

Infectious mononucleosis (IM)

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30
Q

• Signs and symptoms: Fever, malaise, lethargy, sorethroat, and enlarged lymph nodes

A

Infectious mononucleosis (IM)

31
Q

Same as HIV

A

Infectious mononucleosis (IM)

32
Q

EBV Primary target cells

A

B cells

33
Q

EBV Hallmark

A

Downey cells

34
Q

Entry gained following attachement to the complement receptor 2 (CR2)

A

B cells

35
Q

Virus infected B cells which has T cells characteristics

A

Downey cells

36
Q

Enlarged lymphocytes with atypical nuclei

A

Downey cells

37
Q

Specific EBV antigens

A
  1. Viral capsid antigens (VCA)
  2. EBV nuclear protein (EBNA)
  3. Early antigen (EA)
38
Q

Found in patients with acute phase IM

A
  1. Viral capsid antigens (VCA)
39
Q

Found in cases of IM but they disappear early after recovery

A

Early antigen (EA)

40
Q

Early antigen (EA) Diagnostic procedure:

A

EA-Diffuse
EA-Restricted

41
Q

EA-Diffuse:

A

Nucleus and Cytoplasm of B cells

42
Q

EA-Restricted:

A

Cytoplasm only of B cells

43
Q

SEROLOGIC TESTS FOR EBV

A

• Most specific: EBV DNA PCR
• Heterophile antibody tests
• EBV specific serology

44
Q

Heterophile antibody tests Ex

A
  1. Monospot
  2. Paul Bunnel test
  3. Davidsohn Differential
45
Q

Detects the presence of IgM and IgG

A

• EBV specific serology

46
Q

• EBV specific serology types

A
  1. EBV VCA IgM and EBV VCA IgG
  2. EBNA IgG
47
Q

Principle: Direct immune agglutination

A

Monospot test

48
Q

Reagent: Horse RBCs agglutinate with the heterophile Ab present in EBV

A

Monospot test

49
Q

• Principle: Direct immune agglutination

A

Paul-Bunnel test

50
Q

• Reagent: Sheep RBCs agglutinate with the heterophile Ab

A

Paul-Bunnel test

51
Q

• Titer of 1:56 is positive presumption test of IM

A

Paul-Bunnel test

52
Q

• Principle: Absorption test

A

Davidsohn Differential Test

53
Q

Davidsohn Differential Test
Reagent:
Unknown:

A

Reagent: Sheep RBCs
Unknown: Serum (heterophile Abs)

54
Q

Davidsohn Differential Test
1. Perform [?]
2. Allow to be absorbed by [?]
3. Distinguish whether [?] based whether the agglutinins were absorbed.

A

Paul Bunnel test (Serum +Sheep RBC = heterophile sheep agglutinins)

guinea pig kidney cells and beef erythrocytes

Forssman Ag (Guinea pig kidney), serum sickness and IM

55
Q

Absorbed by Guinea pig Kidney cells

A

FORSSMAN
SERUM SICKNESS

56
Q

Absorbed by Beef (Ox) Erythrocytes

A

IM
SERUM SICKNESS

57
Q

Mnenomic: GF BI SS (GirlFriend Believes In Safe Sex)

A
58
Q

• G- guinea pig (+) →

A

Forssman

59
Q

• B- beef erythrocyte (+) →

A

IM

60
Q

• SS-serum sickness (

A

both +)

61
Q

Not a virus, but an acute phase reactant; rises in inflammation

A

C-REACTIVE PROTEIN

62
Q

Abnormal Protein that appears in the acute stages of various

A

C-REACTIVE PROTEIN

63
Q

Non-specific indicator of the ff. disorders:

A

Bacterial infections
Acute rheumatic fever
Malignant diseases
Viral infections
TB
MI
Burn

64
Q

CRP

Name is derived from the formation of precipitate when reacted with

A

somatic C polysaccharide of pneumococcus

65
Q

CRP Electrophoretic mobility:

A

gamma region

66
Q

CRP Synthesized:

A

Liver

67
Q

CRP Thermolabile :

A

70C for 30 minutes

68
Q

Rapid slide test Principle:

A

reverse passive agglutination

69
Q

Rapid slide test
Unknown:
Reagent:

A

Unknown: Antigen (CRP)
Reagent: Anti-CRP Antibody

70
Q

Rapid slide test

(+) Result:

A

Agglutination (presence of CRP)

71
Q

Patient Serum (Ag) is mixed with latex particles coated with anti-CRP antibody

A

Rapid slide test

72
Q

CRP Serologic Assay

A

Rapid slide test

73
Q

Advantage of CRP Assay

More sensitive indicator inflammatory process than:

A

Erythrocyte Sedimentation Rate (ESR)
WBC count

74
Q

Disadvantage of CRP Assay

A

Non-specific (positive for ALL inflammatory diseases)
Lack of correlation between positivity of the test and severity

IMMUNE 1 LEC (28 decks)

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