microbiology (bacteria) Flashcards
Information regarding bacteria that may come up as part of microbiology.
bacterial shapes
cocci
bacilli
vibrio
spirochaete
ziehl-neelsen staining
used for Mycobacteria
acid-fast will stain pink/red
non acid-fast will stain blue
catalase test
staphylococci is catalase positive (will generate gas bubbles); streptococci is catalase negative (no reaction)
coagulase test
Staphylococcus aureus is coagulase positive (will clot when added to plasma); other Staphylococcus spp. are coagulase negative
oxidase test
bacteria that produce cytochrome oxidase are oxidase positive - e.g. Pseudomonas and Neisseria
positive result will turn black/purple in colour
optochin test
differentiates Strep. pneumonia from other alpha haemolytic Streptococci
Streptococci pneumonia + optochin will cause lysis
metronidazole
identifies sensitive anaerobes by inhibiting their growth on culture medium
MacConkey agar
isolates and identifies enteric bacteria
contains bile salts, lactose, and pH indicator
lactose fermenting bacteria will cause red/pink colony - Escherichia coli and Klebsiella
non-lactose fermenting bacteria will leave a clear colony - Salmonella, Shigella, and Pseudomonas
blood agar - haemolysis
alpha haemolysis - green - partial RBC and Hb breakdown - viridans
beta haemolytic - clear zones - complete erythrocyte lysis
non-haemolytic - no change
Lancefield grouping
differentiates between beta haemolytic Strep by detecting surface antigens
group A: Streptococcus pyogenes
group B: Stretococcus agalactiae
group D: Enterococcus
exotoxin
produced mostly by gram +ve bacteria
actively secreted toxins with a specific action (e.g. botulinum toxin)
endotoxin
a component of the cell wall that is released when bacteria are damaged
gram +ve: lipoteichoic acid
gram -ve: lipopolysaccharides
less specific actions include septic shock
gram positive bacterial groups
Streptococcus Staphylococcus Corynebacterium Listeria Bacillus Clostridium
Streptococcus
chains or diplococci gram +ve catalase negative alpha haemolytic: oralis, sanguis, mutans, mitis beta haemolytic: progenies, agalactiae non-haemolytic: Enterococcus faecalis Aerobic
Streptococcus pneumoniae
alpha haemolytic, diplococci, commensal of upper respiratory tract in 10-30%, droplet transmission
CAP, COPD exacerbation, sinusitis, otitis media
bacterial meningitis
treat with penicillin or erythromycin
Streptococcus Viridans (alpha haemolytic)
upper respiratory tract and GI tract commensal
bacterial endocarditis
dental caries
treat with penicillin/amoxicillin (erythromycin if allergic)
bacterial endocarditis treat with penicillin and gentomycin
Streptococcus pyogenes (group A ß-haemolytic)
upper respiratory tract commensal in 5%
transmission: airborne, respiratory secretions, hands
skin: cellulitis, necrotising fasciitis, impetigo, erysipelas, wound infections, scarlet fever
URTIs: pharyngitis (strep throat), tonsillitis, acute otitis media
invasive: sepsis
post-infective: rheumatic fever and glomerulonephritis
treat with penicillin/amoxicillin (erythromycin if allergic)
Streptococcus agalactiae (group B ß-haemolytic)
faecal commensal in 30-40% of population and vaginal in 10-30%
causes neonatal septicaemia and meningitis in children
causes UTIs, post-partum sepsis, bacterial endocarditis, and septicaemia in adults
Enterococcus (group D non-haemolytic, MacConkey growth)
GI tract commensal infective endocarditis UTI wound infections IV catheter infection treat with penicillin/vancomycin treat IE with penicillin/vancomycin + gentamicin
Staphylococcus
cocci in clumps
gram positive
catalase positive
S.aureus is coagulase positive; the others are coagulase negative
S.aureus has golden yellow colonies; S.epidermidis and S.saprophyticus have white colonies
aerobic
Staphylococcus aureus
coagulase positive
anterior nares in 20-30% of population
impetigo, paronychia, abscesses, cellulitis, wound/IV line infection
osteomyelitis and septic arthritis
septicaemia, conjunctivitis, IE, pneumonia
toxin mediated: toxic shock syndrome, scalded skin syndrome, and food poisoning
flucloxacillin
vancomycin for MRSA
Staphylococcus epidermidis
coagulase negative
skin and mucous membranes
foreign body infection or native valve endocarditis
treat with flucloxacillin (or vancomycin if resistant)
Staphylococcus saprophyticus
coagulase negative
UTIs in sexual active young women
treat with trimethoprim or flucloxacillin
Corynebacterium
bacilli
gram positive
aerobic
e.g. C.diptheriae
Corynebacterium diphtheriae
nasopharyngeal carriers
droplet spread
diphtheria toxin –> epithelial cell destruction and myocardial/neural cell damage –> bull neck lymphadenopathy and pseudomembrane covering tonsils
treat with erythromycin, diphtheria antitoxin and vaccination
less than 5 cases per year in UK (due to vaccination)
Listeria
bacilli
gram positive
aerobic
e.g. Listeria monocytogenes
Listeria monocytogenes
domestic animal faeces and food
transmission: contact with animals, ingestions, transplacentally, perinatally
miscarriage and stillbirth
can cause meningitis, pneumonia, encephalitis, and sepsis in neonates, the elderly, or the immunocompromised
treat with ampicillin/erythromycin and gentamicin
Bacillus
bacilli gram positive spore forming aeorbic e.g. Bacillus anthracis, B. cereus
Bacillus anthracis
infected herbivore carcasses
transmission from handling infected materials (not from eating)
cutaneous lesions
anthrax may also be pulmonary or gastrointestinal
treat with ciprofloxacin or doxycycline