lab 8 bone marrow Flashcards
In general, cells of the ____ series have round nuclei with dense, darkly stained, clumped chromatin and basophilic cytoplasm.
erythroid
As the ___ cell matures, the cytoplasm becomes hemoglobinized, i.e., more acidophilic and less basophilic, while the nucleus condenses, becomes smaller and is eventually extruded
erythroid
granulocytes as they mature
The cell nucleus is lighter purple and has a finer chromatin pattern.
The nuclear shape becomes successively flattened, eccentric, elongated and lobulated. The cytoplasm is initially basophilic and granular, but the intensity of basophilia decreases with maturation.
another name for Basophilic Erythroblast
Prorubricyte
another name for proeyrthoblast/ pronormoblast
Rubriblast
RBC maturation
what? and where
what
Rubriblast and Prorubricyte
proerythroblast, basophilic erythroblast
large cells with a darkly staining, round, centrally-located nucleus containing coarseand clumped chromatin
the cytoplasm is very basophilic (navy blue) and smooth.
The rubriblast(proerythroblast) differs from the prorubricyte(basophilic erythroblast) in that a prominent ____ will be present
nucleolus
a nucleolus appears as a different colored spot (typically blue) within the more purple staining nucleus.
Rubricyte
Polychromatophilic Erythroblast
rubricyte/ (Polychromatic Erythroblast)
This cell is smaller than the prorubricyte (Basophilic Erythroblast)
the nucleus: denser chromatin and it occupies a smaller portion of the cell.
Hemoglobin synthesis imparts a pink color, here and there, overall mottled or lavender appearance (polychromasia).
Metarubricyte or
Normoblast (Orthochromatic Erythroblast)
Normoblast (Orthochromatic Erythroblast) (metarubricyte)
smaller than a rubricyte. (Polychromatic Erythroblast)
Its nucleus contains very condensed (almost pyknotic) chromatin and is darkly stained.
Cytoplasm: pink or at least lighter in color due to the increased hemoglobin content.
Polychromatophil or
(Reticulocyte)
Polychromatophilic erythrocyte
Polychromatophil (Reticulocyte):
immature, anucleate red blood cell.
Cytoplasm is more lavender than a mature erythrocyte because of a decreased hemoglobin content.
A reticulocyte count is used to ____
assess regenerative response in anemic patients.
RBC terms per handout
Rubriblast –Proerythroblast
Prorubricyte –basophilic erythroblast
Rubricyte –polychromatic erythroblast
Metarubricyte–orthochromatic erythroblast
Polychromatophil -reticulocyte (polychromatophilic RBC)
Erythrocyte
Myeloid seriers
Myeloblast Progranulocyte (Promyelocyte) Myelocyte Metamyelocyte Band Cell Mature Segmented Granulocytes
Large cell with a round to oval nucleus and finely dispersed chromatin - euchromatic. Nucleoli may be present. The cytoplasm is basophilic and grainy.
myeloblast (hard to find)
In this cell, the nuclear chromatin is beginning to clump. Magenta or purple (azurophilic) granules are visible in the basophilic cytoplasm. Neutrophils, basophils, and eosinophils are indistinguishable up to and including this stage of development.
Promyelocyte)
smaller than the progranulocyte. Its nucleus is usually oval and eccentrically situated. The nuclear chromatin is coarser. Specific granules develop in the cytoplasm.
Myelocyte.
The nucleus begins to indent to form lobes (sausage- shaped) and the chromatin becomes denser. The cytoplasm is paler blue.
Metamyelocyte
As the nucleus matures, it continues to indent to form a ____ or rod shape. The nucleus eventually becomes segmented in appearance. The cytoplasm is pink and specific granules may be seen.
band
Band Cells and Mature Segmented Granulocytes.
stained with Giemsa
The erythroid precursor cells stain___ than the granulocytic cells and they are less ____
darker
numerus
Difference between bone marrow of mammal and bird:
As in mammals, myelopoiesis occurs in the space outside of the vessels of the marrow.
In avian species: erythropoiesis takes place within the blood vessels of the marrow. The earliest red blood cell precursors are in close association with the endothelium of the vessel wall. As the cells mature, they move out towards the center.
bone marrow in birds
myeloid series
Mature megakaryocyte
Immature megakaryocyte
bone marrow
- Mature megakaryocyte
Early prorubricyte (Basophilic Erythroblast)
Rubricytes (Polychromatic Erythroblast)
- Rubricyte (Polychromatic Erythroblast)
Metarubricyte (Orthochromatic Erythroblast)
Metarubricyte (Orthochromatic Erythroblast) top cell
Rubricyte bottom cell
Progranulocyte, late “a better example would have a centrally located nucleus.
Metamyelocyte (with indented nucleus) & myelocyte (with oval nucleus)
Metamyelocyte
Band cell
Band cell
megakaryocyte
osteoclast
erythroid cell
granulocytic cells
Polychromatophilic Erythroblast (Rubricyte)
Basophilic Erythroblast (Prorubricyte)
Orthochromatophilic Erythroblast (Metarubricyte/Normoblast)
Promyelocyte
Progranulocyte
Basophilic Myelocyte
Neutrophilic
Myelocyte
Late Neutrophilic Metamyelocyte
Neutrophilic Band Cell
cat bone marrow
plastic section
