Lab 1

Question 1

Ubiquitous

Answer 1

Everwhere

Question 2

Binary fission

Answer 2

divide in two

Question 3

Media

Answer 3

Plural, nutritious food used to grow microoganisms

Question 4

Medium

Answer 4

Singular, nutritious food used to grow microorganisms

Question 5

Culture

Answer 5

bacterial growth in a medium

Question 6

Broth

Answer 6

Nutritious liquid

Question 7

Agar

Answer 7

a seaweed extract

Question 8

turbidity

Answer 8

cloudiness

Question 9

Colony

Answer 9

visible mass of cells descended from the original

Question 10

Two important criteria to keep in mind while growing microorganisms in the lab

Answer 10

Temperature and oxygen requirements

Question 11

Obligate aerobes

Answer 11

Bacteria that require oygen

Question 12

Obligate anaerobes

Answer 12

Microbes that grow only in the absence of air

Question 13

Facultative anaerobes

Answer 13

Bacteria that prefer to grow in the presence of air but can grow anaerobically if the conditions change

Question 14

Pure culture

Answer 14

A microbial culture in which only one strain or clone of the desired organisms is present

Question 15

Aseptic technique

Answer 15

Sterilize everything that is to encounter the culture before use, and then once it’s touched a culture it’s sterilized again before putting it on the lab bench or disposing

Question 16

Why use a spread plate technique?

Answer 16

Spread out a diluted culture of microorganism so that you get spreading in which individual colonies can grow on solid media

Question 17

What result do we hope for when we use the spread plate technique?

Answer 17

Quantitation of samples

Question 18

What is the purpose of the streak plate technique?

Answer 18

Obtain a pure culture

Question 19

What is a pure culture?

Answer 19

Bacteria derived from a single cell and therefore genetically alike