9.23.2022. + 9.26.2022. Study Questions (part 2) Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

phage reproduction either leads to… (2)

A

productive infection or latent state

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

describe the end results of a productive infection?

A

some kill the host, others don’t

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

a temperate phage either leads to…

A

lytic infection or lysogenic infection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

whether prophage persists or lytic cycle begins depends on…

A

repressor and other regulatory proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

repressor and other regulatory proteins determine…

A

whether prophage persists or lytic cycle begins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is a prophage? (2)

A
  • latent form of a temperate phage
  • phage DNA usually inserted into host chr
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

the latent form of a temperate phage is called…

A

prophage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what is a temperate phage?

A

bacteriophage that either produces a 1. productive infection or 2. latent infection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what does a repressor do?
why is a repressor important?

A
  • prevents expression of genes required for excision
  • essential for maintaining lysogenic (latent) state
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

lysogenic state, aka

A

latent state

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

latent state, aka

A

lysogenic state

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

how to excise phage DNA from bacterial DNA?

A

phage induction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what is phage induction?

A

a way to excise phage DNA from bacterial DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what does phage induction accomplish?

A

lets phage escape from damaged host

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

how does phage induction work? (4)
DREP

A

DREP
1. damage lysogenic culture with UV light
2. SOS repair system responds to damage by activating enzyme that destroys repressor
3. with repressor gone, prophage is excised from chr., allowing phage to enter lytic cycle, and
4. a productive infection results

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what does a plaque assay do?

A

determine concentration of phage particles in samples like sewage, seawater, and soil

17
Q

how does a plaque assay work?

A
18
Q

why use a plaque assay? (2)

A
  • grow phages in the lab
  • count the # of plaques to determine the titer
19
Q

what is a titer?

A

concentration of infectious phage particles in the original phage suspension