10.19.2022. IS & Study Questions

Question 1

What are two ways in which a disease can be diagnosed using immunoassays?

Answer 1

testing serum or plasma

Question 2

Define seroconversion

Answer 2

change from serum that lacks specific antibodies (seronegative) to serum that has those antibodies (seropositive)

Question 3

What is the purpose of the anti-human IgG in an indirect test?

Answer 3

they bind to any human IgG molecules, regardless of their source or specificity to detect any bound IgG antibodies (primary antibodies)

Question 4

What does a direct test typically look for?

Answer 4

typically used to identify unknown antigens

Question 5

What does an indirect test typically look for?

Answer 5

typically used to detect antibodies of a given specificity in a patient’s serum. uses labeled secondary antibodies to detect unlabeled primary antibodies bound to a given antigen.

Question 6

Give two situations that would cause a person to generate protective antibodies, aka seroconvert?

Answer 6

• not exposed to pathogen, lacks antibodies, seronegative. is infected, produces antibodies, becomes seropositive.

Question 7

What result will be seen for a positive test sample if a technician forgets to add an anti-human IgG into an indirect test?

Answer 7

you wouldn’t see the labeled secondary antibodies which indicate a positive test since there are none so you would think it’s a negative test

Question 8

What result will be seen for a negative sample if a technician forgets to add an anti-human IgG into an indirect test?

Answer 8

nothing. negative result is characterized by the label not being detected.

Question 9

what is serum?

Answer 9

the fluid portion of blood that remains after the blood clots

Question 10

what is plasma?

Answer 10

the fluid portion of blood treated with an anticoagulant to prevent clotting