Glycolysis

Question 1

what is niacin?

Answer 1

vitamin B3

Question 2

what does vitamin B3 provide in the process of glycolysis?

Answer 2

the nicotinamide part of NAD+ molecule

- needs to be provided in diet

Question 3

what can a lack of naicin cause?

Answer 3

\+ pellagra
\+ sensitivity to sunlight
\+ dermatitis
\+ alopecia
\+ glossitis
\+ weakness
\+ ataxia

Question 4

what happens to NAD+ under aerobic conditions?

Answer 4

regenerated:

- NADH passes electrons and H atoms through electron transfer chain

Question 5

in anaerobic conditions, how is NAD+ regenerated?

Answer 5

NADH re-oxidised by reducing pyruvate to lactate

Question 6

why is the enzyme, phosphofructokinase, the most important point at which glycolysis is regulated?

Answer 6

1. High [ATP] allosterically inhibits the enzyme
2. Low pH inhibits the enzyme (lactate accumulation)
3. High [citric acid] inhibits
4. High [fructose-6-P] stimulates the enzyme

Question 7

what does phosphofructokinase do?

Answer 7

Catalyses:
fructose-6-P
into
fructose-1,6-bisphosphate