Genetics Flashcards

1
Q

Purines

A
  • Adenine

- Guanine

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2
Q

Pyrimidine

A
  • Uracil
  • Cytosine
  • Thymine
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3
Q

Which NT bonding is more stable?

A

GC 3 H bonds

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4
Q

Denaturation

A

Separation of strands by breaking H bonds

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5
Q

Annealing

A

Coming together of strands by forming H bonds

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6
Q

Hybridization

A

Formation of ds by complementary base pairings of two ss

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7
Q

DNA supercoiling

A

Twisting of dsDNA allows for packing

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8
Q

Toposiomerase I

A

Cleaves one strand of DNA to cause relaxation

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9
Q

Topoisomerase II

A

Cleaves both strand to cause supercoiling (DNA Gyrase)

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10
Q

DNA Gyrase

A

Cleave both strands of of DNA and a target for certain antibiotics

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11
Q

Drugs that target DNA gyrase

A
  • Quinolone
  • Fluoroquinolone (enrofloxacin)
  • Novobiocin
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12
Q

Copy number

A

Number of plasmids per cell

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13
Q

Transposons

A

Jumping genes. Genes that move from site to site on the same or different chromosome

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14
Q

Why are transposons important

A

Can move antibiotic resistance to different bacteria and allow spreading of resistance if introduced into plasmids or viruses

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15
Q

Types of transposons

A
  • Insertion sequences (code for transposase gene)

- Transposons

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16
Q

What are bacteriophages

A

Viruses that infect bacteria. Can be DNA or RNA, ds or ss

17
Q

Types of bacteriophages

A
  • Lytic

- Lysogenic

18
Q

Which bacteriophage causes immediate syntheses of bacteriophage DNA?

A

Lytic

19
Q

What bacteriophage cycle incorporates its DNA into the bacteria and then divides through cell division?

A

Lysogenic

20
Q

Phage conversion

A

Presence of prophage confers new properties

21
Q

Phage immunity

A

Presence of prophage prevents infection by the same phage

22
Q

What is phage therapy

A

Therapeutic use of phages to treat bacterial infections could have less affect on normal bacterial flora due to high specificity of the phages. Bacteria can develop resistance to it though

23
Q

Mutation

A

Changes in NT that is inherited. Can be good or bad

24
Q

Recombination

A

Genes from two different genomes combine

25
Q

Vertical gene transfer

A

Mother to daughter cell recombination

26
Q

Horizontal gene transfer

A

Donor to recipient cell recombination

27
Q

Auxotroph mutant

A

Mutant that lacks ability to make required nutrient and needs it to be provided

28
Q

Point mutation

A

Change in single base or NT. Phenotypic change dependent on where it is

29
Q

Frameshift mutation

A

Insertion or deletion of several bp

30
Q

What are the mechanisms of genetic recombination

A
  • Transformation
  • Transduction
  • Conjugation
31
Q

Transformation

A

incorporation of free DNA into recipient

32
Q

Competent bacteria

A

Able to be transformed

33
Q

Transduction

A

DNA transfer by bacteriophage. Can be generalized or specialized

34
Q

Conjugation

A

DNA transfer from cell to cell contact mediated by conjugative plasmid cell. Donor cell has the conjugated plasmid that it gives to recipient