Genetics Flashcards
Purines
- Adenine
- Guanine
Pyrimidine
- Uracil
- Cytosine
- Thymine
Which NT bonding is more stable?
GC 3 H bonds
Denaturation
Separation of strands by breaking H bonds
Annealing
Coming together of strands by forming H bonds
Hybridization
Formation of ds by complementary base pairings of two ss
DNA supercoiling
Twisting of dsDNA allows for packing
Toposiomerase I
Cleaves one strand of DNA to cause relaxation
Topoisomerase II
Cleaves both strand to cause supercoiling (DNA Gyrase)
DNA Gyrase
Cleave both strands of of DNA and a target for certain antibiotics
Drugs that target DNA gyrase
- Quinolone
- Fluoroquinolone (enrofloxacin)
- Novobiocin
Copy number
Number of plasmids per cell
Transposons
Jumping genes. Genes that move from site to site on the same or different chromosome
Why are transposons important
Can move antibiotic resistance to different bacteria and allow spreading of resistance if introduced into plasmids or viruses
Types of transposons
- Insertion sequences (code for transposase gene)
- Transposons