Exam 3: E. coli

Question 1

Where are Enterobacteriae commonly found

Answer 1

Water, soil but mostly GIT

Question 2

What is the purpose of Enterobacteria in the GIT

Answer 2

1. Absorption of food
2. Waste processing
3. Vit. K production

Question 3

What endotoxin is produced by Enterobacteria that causes endotoxic shock?

Answer 3

Lipid A

Question 4

Are Enterobacteria gram + or -

Answer 4

Gram -

Question 5

What are the 3 coliform bacteria?

Answer 5

1. E. coli
2. Klebsiella
3. Enterobacter

Question 6

What are the 5 Enterobacterium we learned about

Answer 6

1. Escherichia
2. Salmonella
3. Klebsiella
4. Proteus
5. Yersinia

Question 7

What are the two strains of E. coli?

Answer 7

1. Intestinal strains

2. Extra-intestinal Strains

Question 8

What are some things that extra intestinal E. coli cause?

Answer 8

1. Resp
2. Septicaemia
3. UTI
4. Mastitis

Question 9

What are the two categories of E. coli virulence factors?

Answer 9

1. Cell associated

2. Extracellular

Question 10

What are the 4 cell associated virulence factors of E. coli?

Answer 10

1. Endotoxin
2. Capsule
3. Fimbrial
4. Non-fimbrial adhesins

Question 11

What are the 3 Extracellular virulence factors of E. coli

Answer 11

1. Enterotoxins
2. Cytotoxins
3. Siderophores

Question 12

What are the 5 Enteric pathotypes of E. coli

Answer 12

1. Enterotoxigenic (ETEC)
2. Enterohemorrhagic (EHEC)
3. Verotoxigenic (VTEC)
4. Enteropathogenic (EPEC)
5. Rabbit pathogenic (RPEC)

Question 13

What are the 3 Extra-intestinal pathotypes of E. coli

Answer 13

1. Avian pathogenic (APEC)
2. Necrotoxigenic (NTEC)
3. Mastitis causing E. coli
4. MMA in pigs

Question 14

What are the 6 pathotypes of E. coli that affect pigs

Answer 14

1. ETEC
2. VTEC
3. EPEC
4. UTI
5. MMA
6. Septicaemiae

Question 15

Which Pathotype of E. coli causes watery D+ in piglets aged 2-4 weeks

Answer 15

ETEC

Question 16

Why are adhesion factors in ETEC important?

Answer 16

Used for vacc.

Question 17

Where are the receptors for the Adhesion factors of ETC in pigs?

Answer 17

Small intestine

Question 18

What are the 6 adhesion factors of ETEC?

Answer 18

F4
F5
F6
F18
F41
Non-fimbral adhesions

Question 19

What are the two types of Enterotoxins on the plasmids of ETEC?

Answer 19

1. Heat labile

2. Heat stable

Question 20

T/F. There is an equilibrium b/t maternal immunity and infection pressure in ETEC?

Answer 20

T

Question 21

What are the 3 things that cause a disturbance in equilibrium leading to dz in ETEC?

Answer 21

1. Inc. infection pressure
2. Dec Maternal immunity
3. Appearance of new type

Question 22

What are the C/S of ETEC?

Answer 22

1. dehydration
2. Smelly D+
3. inc. thirst

Question 23

How do you Dx ETEC in pigs

Answer 23

1. Agg. test
2. PCR
3. Detection of Virulence factors

Question 24

How do you tx. ETEC in pigs?

Answer 24

Fluids and antimicrobials

Question 25

T/F. There are vacc. available for ETEC

Answer 25

T

Question 26

What strain of E. coli causes Edema Dz in pigs

Answer 26

VTEC

Question 27

Where is Adhesion of VTEC in pigs

Answer 27

Small intestine

Question 28

What are the 3 virulence factors of VTEC in pigs

Answer 28

1. Adhesion 2. Exotoxin 3. Endotoxin

Question 29

What Exotoxin is produced by VTEC in pigs?

Answer 29

VT2E/Stx2e

Question 30

What do endotoxins produced by VTEC cause in pigs?

Answer 30

acute death

Question 31

What Adhesion factor is important in VTEC infections in pigs

Answer 31

F18

Question 32

What are the C/S of VTEC in piglets

Answer 32

1. acute mortality 2. D+ 3. Anorexia 4. Edema 5. Swollen eyelids

Question 33

How do you Dx VTEC in pigs

Answer 33

1. C/S 2. Pathology 3. Bacteriology 4. PCR

Question 34

how do you Tx VTEC in pigs?

Answer 34

Fast and ABx

Question 35

What pathotype of E. coli causes D+ at weaning

Answer 35

EPEC

Question 36

What is used in EPEC infection in piglets for initial attachement

Answer 36

BFP bundle forming pili

Question 37

How do you Dx EPEC in pigs

Answer 37

Isolation and PCR

Question 38

What is the most important pathogen causing UTI in pigs

Answer 38

E. coli

Question 39

DDx of UTI in pigs

Answer 39

Actinobaculum suis

Question 40

Where does E. coli causing UTI in pigs come from?

Answer 40

Intestine

Question 41

Predisposing factors For E. coli UTI in pigs

Answer 41

1. Hygiend 2. Water intake 3. Obstipation 4. Age

Question 42

Symptoms of E. coli UTI in pigs

Answer 42

1. Anorexia 2. Hematuria 3. Vag. exudate 4. General malaise

Question 43

how do you Dx E. coli UTI in pigs

Answer 43

Bacteriology

Question 44

Tx of UTI in pigs

Answer 44

Culling | Abx

Question 45

Disease in pigs secondary to enteric infection

Answer 45

Septicaemiae

Question 46

What are the 3 strains of E. coli that can infect cows

Answer 46

1. ETEC 2. EPEC 3. EHEC 4. NTEC (septicemic)

Question 47

ETEC infects cows what age?

Answer 47

< 3 days old

Question 48

C/S of ETEC in calves

Answer 48

1. Watery D+ | 2. Dehydration

Question 49

What are the 2 virulence factors of ETEC in cows

Answer 49

1. Fimbrae | 2. Toxins

Question 50

What are the fimbriae associated with ETEC in cows

Answer 50

F5 F 41 F17 CS31A

Question 51

What are the two toxins associated with ETEC in calves

Answer 51

Sta and Stb

Question 52

T/F. All ETEC toxins in cattle are heat labile

Answer 52

F. Heat stable only

Question 53

how do you Dx ETEC in catle

Answer 53

Elisa | Culture and AGG. or PCR

Question 54

Which strain of E.coli in cattle is zoonotic?

Answer 54

EHEC

Question 55

Cows 1-8 wks with bloody D+ could be infcted with what 2 strains of E. coli

Answer 55

EHEC and EPEC

Question 56

Where is EHEC and EPEC in cattle found

Answer 56

Lg. intestine

Question 57

Disease in bovine caused by E. coli

Answer 57

Mastitis

Question 58

What are the virulence factors of E. coli Mastitis in cows

Answer 58

None

Question 59

What is the Strain of E. coli that causes Septicaemia in cows

Answer 59

NTEC

Question 60

What are The virulence factors of NTEC which causes septicaemia in cows

Answer 60

1. Serum resistance 2. Fe uptake 3. Endotoxin 4. Capsule 5. Adhesion factors 6. DNF1 and 2 toxins

Question 61

How do you Dx septicaemic E. coli in bovines

Answer 61

C/S | Isolation and PCR

Question 62

How do you tx Septicaemic E. coli in bovines

Answer 62

1. NSAIDS 2. Antibiotics 3. Gamma globulins

Question 63

How do you prevent Septicemic E. coli in cattles

Answer 63

Colostrum

Question 64

Where is E. coli isolated in dogs and cats

Answer 64

Poops

Question 65

What are the two Diseases caused by E. coli infection in dogs

Answer 65

1. Cystitis | 2. Pyometra

Question 66

E. Coli strain in birds

Answer 66

APEC

Question 67

What is the most important bacterial Dz of broilers and layers

Answer 67

APEC

Question 68

What are the specific serotypes of E. coli in poultry

Answer 68

01 02 078

Question 69

T/F. GI signs are the most important C/S in broilers infected with E. coli

Answer 69

F: APEC is Extra enteric, so no GI signs

Question 70

What are the C/S of E. coli infection in birds

Answer 70

1. Septicemia 2. Resp 3. Peritonitis 4. Scabby hip

Question 71

What is the most common C/S of E. coli infection in layers

Answer 71

Peritonitis

Question 72

Dx of E. coli in birds

Answer 72

1. C/S | 2. Isolation

Question 73

What are the 4 DDx of E.coli in birds

Answer 73

1. Yolk rest infection 2. Acute sepsin 3. Coligranuloma 4. Airsacculitis

Question 74

How do you Tx E. coli in birdss

Answer 74

Difficult, so do AST

Question 75

How do you prevent E. coli in birds

Answer 75

1. hygiene 2. Stress 3. Temp. 4. Vacc

Question 76

What 2 serotypes cause Scabby hip in broilers?

Answer 76

078 and 02

Question 77

how do you prevent scabby hip?

Answer 77

Dec. number of animals housed together

Question 78

How do you Dx E. coli in rabits

Answer 78

1. C/S | 2. Isolation and subtyping

Question 79

T/F. Isolation and subtyping is not necessisary in rabbit E. coli infections

Answer 79

F. Necessary! some types are more lethal

Question 80

How do you tx E. coli in rabbits

Answer 80

Abx