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Bacteriology > Exam 3: E. coli > Flashcards

Exam 3: E. coli Flashcards

1
Q

Where are Enterobacteriae commonly found

A

Water, soil but mostly GIT

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2
Q

What is the purpose of Enterobacteria in the GIT

A
  1. Absorption of food
  2. Waste processing
  3. Vit. K production
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3
Q

What endotoxin is produced by Enterobacteria that causes endotoxic shock?

A

Lipid A

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4
Q

Are Enterobacteria gram + or -

A

Gram -

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5
Q

What are the 3 coliform bacteria?

A
  1. E. coli
  2. Klebsiella
  3. Enterobacter
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6
Q

What are the 5 Enterobacterium we learned about

A
  1. Escherichia
  2. Salmonella
  3. Klebsiella
  4. Proteus
  5. Yersinia
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7
Q

What are the two strains of E. coli?

A
  1. Intestinal strains

2. Extra-intestinal Strains

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8
Q

What are some things that extra intestinal E. coli cause?

A
  1. Resp
  2. Septicaemia
  3. UTI
  4. Mastitis
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9
Q

What are the two categories of E. coli virulence factors?

A
  1. Cell associated

2. Extracellular

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10
Q

What are the 4 cell associated virulence factors of E. coli?

A
  1. Endotoxin
  2. Capsule
  3. Fimbrial
  4. Non-fimbrial adhesins
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11
Q

What are the 3 Extracellular virulence factors of E. coli

A
  1. Enterotoxins
  2. Cytotoxins
  3. Siderophores
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12
Q

What are the 5 Enteric pathotypes of E. coli

A
  1. Enterotoxigenic (ETEC)
  2. Enterohemorrhagic (EHEC)
  3. Verotoxigenic (VTEC)
  4. Enteropathogenic (EPEC)
  5. Rabbit pathogenic (RPEC)
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13
Q

What are the 3 Extra-intestinal pathotypes of E. coli

A
  1. Avian pathogenic (APEC)
  2. Necrotoxigenic (NTEC)
  3. Mastitis causing E. coli
  4. MMA in pigs
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14
Q

What are the 6 pathotypes of E. coli that affect pigs

A
  1. ETEC
  2. VTEC
  3. EPEC
  4. UTI
  5. MMA
  6. Septicaemiae
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15
Q

Which Pathotype of E. coli causes watery D+ in piglets aged 2-4 weeks

A

ETEC

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16
Q

Why are adhesion factors in ETEC important?

A

Used for vacc.

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17
Q

Where are the receptors for the Adhesion factors of ETC in pigs?

A

Small intestine

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18
Q

What are the 6 adhesion factors of ETEC?

A 
F4
F5
F6
F18
F41
Non-fimbral adhesions
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19
Q

What are the two types of Enterotoxins on the plasmids of ETEC?

A
  1. Heat labile

2. Heat stable

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20
Q

T/F. There is an equilibrium b/t maternal immunity and infection pressure in ETEC?

A

T

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21
Q

What are the 3 things that cause a disturbance in equilibrium leading to dz in ETEC?

A
  1. Inc. infection pressure
  2. Dec Maternal immunity
  3. Appearance of new type
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22
Q

What are the C/S of ETEC?

A
  1. dehydration
  2. Smelly D+
  3. inc. thirst
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23
Q

How do you Dx ETEC in pigs

A
  1. Agg. test
  2. PCR
  3. Detection of Virulence factors
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24
Q

How do you tx. ETEC in pigs?

A

Fluids and antimicrobials

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25
Q

T/F. There are vacc. available for ETEC

A

T

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26
Q

What strain of E. coli causes Edema Dz in pigs

A

VTEC

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27
Q

Where is Adhesion of VTEC in pigs

A

Small intestine

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28
Q

What are the 3 virulence factors of VTEC in pigs

A
  1. Adhesion
  2. Exotoxin
  3. Endotoxin
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29
Q

What Exotoxin is produced by VTEC in pigs?

A

VT2E/Stx2e

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30
Q

What do endotoxins produced by VTEC cause in pigs?

A

acute death

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31
Q

What Adhesion factor is important in VTEC infections in pigs

A

F18

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32
Q

What are the C/S of VTEC in piglets

A
  1. acute mortality
  2. D+
  3. Anorexia
  4. Edema
  5. Swollen eyelids
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33
Q

How do you Dx VTEC in pigs

A
  1. C/S
  2. Pathology
  3. Bacteriology
  4. PCR
34
Q

how do you Tx VTEC in pigs?

A

Fast and ABx

35
Q

What pathotype of E. coli causes D+ at weaning

A

EPEC

36
Q

What is used in EPEC infection in piglets for initial attachement

A

BFP

bundle forming pili

37
Q

How do you Dx EPEC in pigs

A

Isolation and PCR

38
Q

What is the most important pathogen causing UTI in pigs

A

E. coli

39
Q

DDx of UTI in pigs

A

Actinobaculum suis

40
Q

Where does E. coli causing UTI in pigs come from?

A

Intestine

41
Q

Predisposing factors For E. coli UTI in pigs

A
  1. Hygiend
  2. Water intake
  3. Obstipation
  4. Age
42
Q

Symptoms of E. coli UTI in pigs

A
  1. Anorexia
  2. Hematuria
  3. Vag. exudate
  4. General malaise
43
Q

how do you Dx E. coli UTI in pigs

A

Bacteriology

44
Q

Tx of UTI in pigs

A

Culling

Abx

45
Q

Disease in pigs secondary to enteric infection

A

Septicaemiae

46
Q

What are the 3 strains of E. coli that can infect cows

A
  1. ETEC
  2. EPEC
  3. EHEC
  4. NTEC (septicemic)
47
Q

ETEC infects cows what age?

A

< 3 days old

48
Q

C/S of ETEC in calves

A
  1. Watery D+

2. Dehydration

49
Q

What are the 2 virulence factors of ETEC in cows

A
  1. Fimbrae

2. Toxins

50
Q

What are the fimbriae associated with ETEC in cows

A

F5
F 41
F17
CS31A

51
Q

What are the two toxins associated with ETEC in calves

A

Sta and Stb

52
Q

T/F. All ETEC toxins in cattle are heat labile

A

F. Heat stable only

53
Q

how do you Dx ETEC in catle

A

Elisa

Culture and AGG. or PCR

54
Q

Which strain of E.coli in cattle is zoonotic?

A

EHEC

55
Q

Cows 1-8 wks with bloody D+ could be infcted with what 2 strains of E. coli

A

EHEC and EPEC

56
Q

Where is EHEC and EPEC in cattle found

A

Lg. intestine

57
Q

Disease in bovine caused by E. coli

A

Mastitis

58
Q

What are the virulence factors of E. coli Mastitis in cows

A

None

59
Q

What is the Strain of E. coli that causes Septicaemia in cows

A

NTEC

60
Q

What are The virulence factors of NTEC which causes septicaemia in cows

A
  1. Serum resistance
  2. Fe uptake
  3. Endotoxin
  4. Capsule
  5. Adhesion factors
  6. DNF1 and 2 toxins
61
Q

How do you Dx septicaemic E. coli in bovines

A

C/S

Isolation and PCR

62
Q

How do you tx Septicaemic E. coli in bovines

A
  1. NSAIDS
  2. Antibiotics
  3. Gamma globulins
63
Q

How do you prevent Septicemic E. coli in cattles

A

Colostrum

64
Q

Where is E. coli isolated in dogs and cats

A

Poops

65
Q

What are the two Diseases caused by E. coli infection in dogs

A
  1. Cystitis

2. Pyometra

66
Q

E. Coli strain in birds

A

APEC

67
Q

What is the most important bacterial Dz of broilers and layers

A

APEC

68
Q

What are the specific serotypes of E. coli in poultry

A

01
02
078

69
Q

T/F. GI signs are the most important C/S in broilers infected with E. coli

A

F: APEC is Extra enteric, so no GI signs

70
Q

What are the C/S of E. coli infection in birds

A
  1. Septicemia
  2. Resp
  3. Peritonitis
  4. Scabby hip
71
Q

What is the most common C/S of E. coli infection in layers

A

Peritonitis

72
Q

Dx of E. coli in birds

A
  1. C/S

2. Isolation

73
Q

What are the 4 DDx of E.coli in birds

A
  1. Yolk rest infection
  2. Acute sepsin
  3. Coligranuloma
  4. Airsacculitis
74
Q

How do you Tx E. coli in birdss

A

Difficult, so do AST

75
Q

How do you prevent E. coli in birds

A
  1. hygiene
  2. Stress
  3. Temp.
  4. Vacc
76
Q

What 2 serotypes cause Scabby hip in broilers?

A

078 and 02

77
Q

how do you prevent scabby hip?

A

Dec. number of animals housed together

78
Q

How do you Dx E. coli in rabits

A
  1. C/S

2. Isolation and subtyping

79
Q

T/F. Isolation and subtyping is not necessisary in rabbit E. coli infections

A

F. Necessary! some types are more lethal

80
Q

How do you tx E. coli in rabbits

A

Abx

Bacteriology (31 decks)

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