Exam 3: E. coli Flashcards
Where are Enterobacteriae commonly found
Water, soil but mostly GIT
What is the purpose of Enterobacteria in the GIT
- Absorption of food
- Waste processing
- Vit. K production
What endotoxin is produced by Enterobacteria that causes endotoxic shock?
Lipid A
Are Enterobacteria gram + or -
Gram -
What are the 3 coliform bacteria?
- E. coli
- Klebsiella
- Enterobacter
What are the 5 Enterobacterium we learned about
- Escherichia
- Salmonella
- Klebsiella
- Proteus
- Yersinia
What are the two strains of E. coli?
- Intestinal strains
2. Extra-intestinal Strains
What are some things that extra intestinal E. coli cause?
- Resp
- Septicaemia
- UTI
- Mastitis
What are the two categories of E. coli virulence factors?
- Cell associated
2. Extracellular
What are the 4 cell associated virulence factors of E. coli?
- Endotoxin
- Capsule
- Fimbrial
- Non-fimbrial adhesins
What are the 3 Extracellular virulence factors of E. coli
- Enterotoxins
- Cytotoxins
- Siderophores
What are the 5 Enteric pathotypes of E. coli
- Enterotoxigenic (ETEC)
- Enterohemorrhagic (EHEC)
- Verotoxigenic (VTEC)
- Enteropathogenic (EPEC)
- Rabbit pathogenic (RPEC)
What are the 3 Extra-intestinal pathotypes of E. coli
- Avian pathogenic (APEC)
- Necrotoxigenic (NTEC)
- Mastitis causing E. coli
- MMA in pigs
What are the 6 pathotypes of E. coli that affect pigs
- ETEC
- VTEC
- EPEC
- UTI
- MMA
- Septicaemiae
Which Pathotype of E. coli causes watery D+ in piglets aged 2-4 weeks
ETEC
Why are adhesion factors in ETEC important?
Used for vacc.
Where are the receptors for the Adhesion factors of ETC in pigs?
Small intestine
What are the 6 adhesion factors of ETEC?
F4 F5 F6 F18 F41 Non-fimbral adhesions
What are the two types of Enterotoxins on the plasmids of ETEC?
- Heat labile
2. Heat stable
T/F. There is an equilibrium b/t maternal immunity and infection pressure in ETEC?
T
What are the 3 things that cause a disturbance in equilibrium leading to dz in ETEC?
- Inc. infection pressure
- Dec Maternal immunity
- Appearance of new type
What are the C/S of ETEC?
- dehydration
- Smelly D+
- inc. thirst
How do you Dx ETEC in pigs
- Agg. test
- PCR
- Detection of Virulence factors
How do you tx. ETEC in pigs?
Fluids and antimicrobials
T/F. There are vacc. available for ETEC
T
What strain of E. coli causes Edema Dz in pigs
VTEC
Where is Adhesion of VTEC in pigs
Small intestine
What are the 3 virulence factors of VTEC in pigs
- Adhesion
- Exotoxin
- Endotoxin
What Exotoxin is produced by VTEC in pigs?
VT2E/Stx2e
What do endotoxins produced by VTEC cause in pigs?
acute death
What Adhesion factor is important in VTEC infections in pigs
F18
What are the C/S of VTEC in piglets
- acute mortality
- D+
- Anorexia
- Edema
- Swollen eyelids
How do you Dx VTEC in pigs
- C/S
- Pathology
- Bacteriology
- PCR
how do you Tx VTEC in pigs?
Fast and ABx
What pathotype of E. coli causes D+ at weaning
EPEC
What is used in EPEC infection in piglets for initial attachement
BFP
bundle forming pili
How do you Dx EPEC in pigs
Isolation and PCR
What is the most important pathogen causing UTI in pigs
E. coli
DDx of UTI in pigs
Actinobaculum suis
Where does E. coli causing UTI in pigs come from?
Intestine
Predisposing factors For E. coli UTI in pigs
- Hygiend
- Water intake
- Obstipation
- Age
Symptoms of E. coli UTI in pigs
- Anorexia
- Hematuria
- Vag. exudate
- General malaise
how do you Dx E. coli UTI in pigs
Bacteriology
Tx of UTI in pigs
Culling
Abx
Disease in pigs secondary to enteric infection
Septicaemiae
What are the 3 strains of E. coli that can infect cows
- ETEC
- EPEC
- EHEC
- NTEC (septicemic)
ETEC infects cows what age?
< 3 days old
C/S of ETEC in calves
- Watery D+
2. Dehydration
What are the 2 virulence factors of ETEC in cows
- Fimbrae
2. Toxins
What are the fimbriae associated with ETEC in cows
F5
F 41
F17
CS31A
What are the two toxins associated with ETEC in calves
Sta and Stb
T/F. All ETEC toxins in cattle are heat labile
F. Heat stable only
how do you Dx ETEC in catle
Elisa
Culture and AGG. or PCR
Which strain of E.coli in cattle is zoonotic?
EHEC
Cows 1-8 wks with bloody D+ could be infcted with what 2 strains of E. coli
EHEC and EPEC
Where is EHEC and EPEC in cattle found
Lg. intestine
Disease in bovine caused by E. coli
Mastitis
What are the virulence factors of E. coli Mastitis in cows
None
What is the Strain of E. coli that causes Septicaemia in cows
NTEC
What are The virulence factors of NTEC which causes septicaemia in cows
- Serum resistance
- Fe uptake
- Endotoxin
- Capsule
- Adhesion factors
- DNF1 and 2 toxins
How do you Dx septicaemic E. coli in bovines
C/S
Isolation and PCR
How do you tx Septicaemic E. coli in bovines
- NSAIDS
- Antibiotics
- Gamma globulins
How do you prevent Septicemic E. coli in cattles
Colostrum
Where is E. coli isolated in dogs and cats
Poops
What are the two Diseases caused by E. coli infection in dogs
- Cystitis
2. Pyometra
E. Coli strain in birds
APEC
What is the most important bacterial Dz of broilers and layers
APEC
What are the specific serotypes of E. coli in poultry
01
02
078
T/F. GI signs are the most important C/S in broilers infected with E. coli
F: APEC is Extra enteric, so no GI signs
What are the C/S of E. coli infection in birds
- Septicemia
- Resp
- Peritonitis
- Scabby hip
What is the most common C/S of E. coli infection in layers
Peritonitis
Dx of E. coli in birds
- C/S
2. Isolation
What are the 4 DDx of E.coli in birds
- Yolk rest infection
- Acute sepsin
- Coligranuloma
- Airsacculitis
How do you Tx E. coli in birdss
Difficult, so do AST
How do you prevent E. coli in birds
- hygiene
- Stress
- Temp.
- Vacc
What 2 serotypes cause Scabby hip in broilers?
078 and 02
how do you prevent scabby hip?
Dec. number of animals housed together
How do you Dx E. coli in rabits
- C/S
2. Isolation and subtyping
T/F. Isolation and subtyping is not necessisary in rabbit E. coli infections
F. Necessary! some types are more lethal
How do you tx E. coli in rabbits
Abx