Exam 1: Diagnosis of Bacterial/Fungal Infections Flashcards
Cell Wall component unique to gram Negative bacteria:
a. LPS
b. Teichoic acid
c. Mycolic Acid
d. Peptidoglycan
a. LPS
Gram positive staining in some bacteria is due to the presence of a __
a. Thin peptidoglycan layer
b. teichoic acid
c. Thick peptidoglycan layer
d. Thick outer membrane
C. Thick peptidoglycan layer
Acid Fast Positive staining in mycobacterium is due to the presence of ____
Mycolic acid
If you have already initiated antimicrobial therapy when should you collect a sample?
48 hours after the last dose
A combination of lab practices and techniques, safety equipment and lab facilites to be used to minimize potential exposure.
BSL- bio safety levels
What are the 4 types of culture?
- aerobic culture
- Anaerobic culture
- Microaerophilic culture
- Fungal culture
What are the 2 types of media used for bacteral/fungal cultures?
Broth and agar media
list some examples of broth media
nutrient broth, peptone broth, thioglycolate broth
List some examples of agar media?
- Trypticase soy agar w/ blood
- PEA agar
- MacConkey agar
- Anaerobic media
- Sabourauds dextrose agar
What type of agar would you use to grow gram positive bacteria
PEA agar
What type of agar would you use to grow gram negative bacteria
MacConkey agar
How do you distinquish between Normal flora vs. Pathogen?
C/S and number and type of organism
T/F heavy pure culture of a bacteria is indicative of infection
T
T/F. Isolation of threee or more types of organism is most likely contamination.
T
When is PCR used?
for ID of isolates
