Exam 1: Gram positive Listeria + Erysipelothrix Flashcards

1
Q

Gram positive coccobacilllary rods

A

Listeria

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2
Q

T/F. Listeria are facultative anaerobes

A

T

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3
Q

What type of motility pattern do listeria have?

A

Tumbling motility due to presence of 1-4 flagella

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4
Q

Are Listeria Catalase positive or negative

A

Positive

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5
Q

Are listeria oxidase positive or negative

A

negative

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6
Q

What pH range can listeria tolerate?

A

5.5-9.6

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7
Q

T/F listeria can grow on non-enriched media over a wide range of temp.?

A

T

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8
Q

T/F Listeria cannot grow in the fridge

A

F

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9
Q

Where is listeria commonly found?

A

in the soil and mammalian GIT

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10
Q

T/F Listeria are resistant to harsh environmental conditions?

A

T

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11
Q

What are the 3 main c/s of listeria infection?

A
  1. septicemia
  2. abortion
  3. encephalitis
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12
Q

T/F. Listeria is an obligate intracellular bacteria.

A

F. facultative

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13
Q

Which cells house listeria?

A

Macrophages

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14
Q

T/F. listeria replicate intracellularly

A

T

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15
Q

What does Listeria use internalin A and B used for?

A

adhesion

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16
Q

What does Listeria use Listeriolysin O for?

A

Pore forming cytlytic toxin: destroys memb. of phagosomes

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17
Q

What does listeria use Act A protein for?

A

Actin polymerizing protein

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18
Q

What does listeria usePhospolipase C enzymes for?

A

Degradation of cytoplasmic and endocytic memb.

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19
Q

What are the 3 Listeria species of vet. importance?

A
  1. Listeria monocytogens
  2. Listeria ivanovii
  3. Listeria innocua
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20
Q

Species of Listeria that causes abortion in sheep and cattle?

A

Listeria ivanovii

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21
Q

Type of Listeria that causes abortion in cattle and goats

A

Listeria monocytogenes

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22
Q

Type of Listeria that causes meningoencephalitis in sheep

A

Listeria innocua

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23
Q

Species of Listeria that causes septicemia in birds

A

L. monocytogenes

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24
Q

Type of Listeria that effects pigs

A

L. monocytogenes

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25
Q

What is circling disease

A

Listeria monocytogenes infection in ruminants caused by feeding poor silage

26
Q

What is the pathogenesis of Listeria monocytogenes

A

ingestion or inhalation of listeria monocytogenes via breaks in oral and nasal mucosa

27
Q

What are c/s of circling disease

A
  1. Anorexia
  2. depression
  3. disorientation
  4. walking in circles toward affected side
28
Q

How do you diagnose Listeria monocytogenes?

A

Culture of brain stem

29
Q

T/F. you can vaccinate your herd against Listeria

A

F

30
Q

Listeria monocytogene infection is caused by feeding poor-quality silage with a pH greater than ____.

A

5.5

31
Q

T/F. Pasteruization can terminate Listeria monocytogenes

A

F

32
Q

Gram positive small rods/filaments

A

Erysipelothrix

33
Q

T/F. Erysipelothrix are non-motile facultative anaerobes

A

T

34
Q

Are Erysipelothrix Catalase positive or negative?

A

Negative

35
Q

Are Erysipelothrix oxidase positive or negative?

A

Negative

36
Q

Where is Erysipelothrix commonly found in animals?

A

tonsils and intestines of mammals

Slime layer of fish

37
Q

T/F Erysipelothrix are resistant to harsh environmental conditions

A

T

38
Q

____ are the most important reservoir of Erysipelothrix.

A

swine

39
Q

What 4 syndromes are observed in pigs infected with erysipelothrix?

A
  1. Septicemia
  2. Dermatopathy
  3. arthritis
  4. endocarditis
40
Q

Production of _____ cleaves sialic acid on endothelial cell surface causing vascular damage and hyaline thrombus formation in animals infected with erysipelothrix.

A

Neuroaminidase

41
Q

How is erysipelothrix excreted?

A

oronasal and fecal secretions

42
Q

Virulence factor of Erysipeothrix that provides protection against phagocytosis and allows intracellular rep.

A

Polysaccharide capsule

43
Q

Virulence factor of Erysipelothrix that allows for adherence and invasion of endothelial cells

A

Neuraminidase

44
Q

Virulence factor of Erysipelothric that allows for dissemination of bacteria within tissues

A

Hyaluronidase

45
Q

Virulence factor of Erysipelothrix that allows adhesion

A

SpaA surface protein

46
Q

What are the 2 species of Erysipelothrix important in vet med?

A
  1. E rhusiopathiae

2. E. tonsallarum

47
Q

Which type of Erysipelothrix infects dogs?

A

E. tonsallarum

48
Q

Which species of Erysipelothric infects pigs, turkeys, and sheep

A

E rhusiopathiae

49
Q

Erysipelothrix rhusiopathiae infection in pigs is known as?

A

swine erysipelas or Diamond skin disease

50
Q

WHat are the 3 forms of swine erysipelas?

A
  1. acute
  2. subacute
  3. chronic
51
Q

Pathognomic lesions of swine erysipelas

A

diamond shaped skin lesion

52
Q

Why are pigs 3mo to 1 yr of age most susceptible to erysipelas infections?

A

< 3mo = protected by maternal ab.

>3yr protective active immunity through exposure

53
Q

T/F. Erysipelas infections can be triggered by stress

A

T

54
Q

What are 3 methods of diagnosing Erysipelas in swine

A
  1. skin lesions
  2. Isolation of E. rhusiopathiae
  3. PCR
55
Q

Erysipelas in turkeys is cause by ____

A

Erysipelohrix rhusiopathiae

56
Q

What is acute form Erysipelothric rhusiopathiae in turkeys characterized by?

A

septicemia and sudden death

57
Q

What is chronic form Erysipelas in turkeys characterized by?

A

endocarditis and wt. loss

58
Q

Non-suppurative polyarthritis in lambs is cause by infection of?

A

Erysipelothrix rhusiopathiae

59
Q

How does Erysipelothrix rhusiopathiae organisms enter lambs?

A

through umbilicus or castration wounds

60
Q

T/F. Humans can also get erysipelas?

A

T

61
Q

Human erysipelas is an infectious skin disease cause by ________.

A

Group A beta-hemolytic streptococci