Exam 1: Gram positive Listeria + Erysipelothrix Flashcards

1
Q

Gram positive coccobacilllary rods

A

Listeria

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2
Q

T/F. Listeria are facultative anaerobes

A

T

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3
Q

What type of motility pattern do listeria have?

A

Tumbling motility due to presence of 1-4 flagella

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4
Q

Are Listeria Catalase positive or negative

A

Positive

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5
Q

Are listeria oxidase positive or negative

A

negative

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6
Q

What pH range can listeria tolerate?

A

5.5-9.6

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7
Q

T/F listeria can grow on non-enriched media over a wide range of temp.?

A

T

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8
Q

T/F Listeria cannot grow in the fridge

A

F

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9
Q

Where is listeria commonly found?

A

in the soil and mammalian GIT

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10
Q

T/F Listeria are resistant to harsh environmental conditions?

A

T

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11
Q

What are the 3 main c/s of listeria infection?

A
  1. septicemia
  2. abortion
  3. encephalitis
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12
Q

T/F. Listeria is an obligate intracellular bacteria.

A

F. facultative

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13
Q

Which cells house listeria?

A

Macrophages

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14
Q

T/F. listeria replicate intracellularly

A

T

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15
Q

What does Listeria use internalin A and B used for?

A

adhesion

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16
Q

What does Listeria use Listeriolysin O for?

A

Pore forming cytlytic toxin: destroys memb. of phagosomes

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17
Q

What does listeria use Act A protein for?

A

Actin polymerizing protein

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18
Q

What does listeria usePhospolipase C enzymes for?

A

Degradation of cytoplasmic and endocytic memb.

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19
Q

What are the 3 Listeria species of vet. importance?

A
  1. Listeria monocytogens
  2. Listeria ivanovii
  3. Listeria innocua
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20
Q

Species of Listeria that causes abortion in sheep and cattle?

A

Listeria ivanovii

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21
Q

Type of Listeria that causes abortion in cattle and goats

A

Listeria monocytogenes

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22
Q

Type of Listeria that causes meningoencephalitis in sheep

A

Listeria innocua

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23
Q

Species of Listeria that causes septicemia in birds

A

L. monocytogenes

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24
Q

Type of Listeria that effects pigs

A

L. monocytogenes

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25
What is circling disease
Listeria monocytogenes infection in ruminants caused by feeding poor silage
26
What is the pathogenesis of Listeria monocytogenes
ingestion or inhalation of listeria monocytogenes via breaks in oral and nasal mucosa
27
What are c/s of circling disease
1. Anorexia 2. depression 3. disorientation 4. walking in circles toward affected side
28
How do you diagnose Listeria monocytogenes?
Culture of brain stem
29
T/F. you can vaccinate your herd against Listeria
F
30
Listeria monocytogene infection is caused by feeding poor-quality silage with a pH greater than ____.
5.5
31
T/F. Pasteruization can terminate Listeria monocytogenes
F
32
Gram positive small rods/filaments
Erysipelothrix
33
T/F. Erysipelothrix are non-motile facultative anaerobes
T
34
Are Erysipelothrix Catalase positive or negative?
Negative
35
Are Erysipelothrix oxidase positive or negative?
Negative
36
Where is Erysipelothrix commonly found in animals?
tonsils and intestines of mammals | Slime layer of fish
37
T/F Erysipelothrix are resistant to harsh environmental conditions
T
38
____ are the most important reservoir of Erysipelothrix.
swine
39
What 4 syndromes are observed in pigs infected with erysipelothrix?
1. Septicemia 2. Dermatopathy 3. arthritis 4. endocarditis
40
Production of _____ cleaves sialic acid on endothelial cell surface causing vascular damage and hyaline thrombus formation in animals infected with erysipelothrix.
Neuroaminidase
41
How is erysipelothrix excreted?
oronasal and fecal secretions
42
Virulence factor of Erysipeothrix that provides protection against phagocytosis and allows intracellular rep.
Polysaccharide capsule
43
Virulence factor of Erysipelothrix that allows for adherence and invasion of endothelial cells
Neuraminidase
44
Virulence factor of Erysipelothric that allows for dissemination of bacteria within tissues
Hyaluronidase
45
Virulence factor of Erysipelothrix that allows adhesion
SpaA surface protein
46
What are the 2 species of Erysipelothrix important in vet med?
1. E rhusiopathiae | 2. E. tonsallarum
47
Which type of Erysipelothrix infects dogs?
E. tonsallarum
48
Which species of Erysipelothric infects pigs, turkeys, and sheep
E rhusiopathiae
49
Erysipelothrix rhusiopathiae infection in pigs is known as?
swine erysipelas or Diamond skin disease
50
WHat are the 3 forms of swine erysipelas?
1. acute 2. subacute 3. chronic
51
Pathognomic lesions of swine erysipelas
diamond shaped skin lesion
52
Why are pigs 3mo to 1 yr of age most susceptible to erysipelas infections?
< 3mo = protected by maternal ab. | >3yr protective active immunity through exposure
53
T/F. Erysipelas infections can be triggered by stress
T
54
What are 3 methods of diagnosing Erysipelas in swine
1. skin lesions 2. Isolation of E. rhusiopathiae 3. PCR
55
Erysipelas in turkeys is cause by ____
Erysipelohrix rhusiopathiae
56
What is acute form Erysipelothric rhusiopathiae in turkeys characterized by?
septicemia and sudden death
57
What is chronic form Erysipelas in turkeys characterized by?
endocarditis and wt. loss
58
Non-suppurative polyarthritis in lambs is cause by infection of?
Erysipelothrix rhusiopathiae
59
How does Erysipelothrix rhusiopathiae organisms enter lambs?
through umbilicus or castration wounds
60
T/F. Humans can also get erysipelas?
T
61
Human erysipelas is an infectious skin disease cause by ________.
Group A beta-hemolytic streptococci