Exam 1: Gram positive Listeria + Erysipelothrix Flashcards
Gram positive coccobacilllary rods
Listeria
T/F. Listeria are facultative anaerobes
T
What type of motility pattern do listeria have?
Tumbling motility due to presence of 1-4 flagella
Are Listeria Catalase positive or negative
Positive
Are listeria oxidase positive or negative
negative
What pH range can listeria tolerate?
5.5-9.6
T/F listeria can grow on non-enriched media over a wide range of temp.?
T
T/F Listeria cannot grow in the fridge
F
Where is listeria commonly found?
in the soil and mammalian GIT
T/F Listeria are resistant to harsh environmental conditions?
T
What are the 3 main c/s of listeria infection?
- septicemia
- abortion
- encephalitis
T/F. Listeria is an obligate intracellular bacteria.
F. facultative
Which cells house listeria?
Macrophages
T/F. listeria replicate intracellularly
T
What does Listeria use internalin A and B used for?
adhesion
What does Listeria use Listeriolysin O for?
Pore forming cytlytic toxin: destroys memb. of phagosomes
What does listeria use Act A protein for?
Actin polymerizing protein
What does listeria usePhospolipase C enzymes for?
Degradation of cytoplasmic and endocytic memb.
What are the 3 Listeria species of vet. importance?
- Listeria monocytogens
- Listeria ivanovii
- Listeria innocua
Species of Listeria that causes abortion in sheep and cattle?
Listeria ivanovii
Type of Listeria that causes abortion in cattle and goats
Listeria monocytogenes
Type of Listeria that causes meningoencephalitis in sheep
Listeria innocua
Species of Listeria that causes septicemia in birds
L. monocytogenes
Type of Listeria that effects pigs
L. monocytogenes
What is circling disease
Listeria monocytogenes infection in ruminants caused by feeding poor silage
What is the pathogenesis of Listeria monocytogenes
ingestion or inhalation of listeria monocytogenes via breaks in oral and nasal mucosa
What are c/s of circling disease
- Anorexia
- depression
- disorientation
- walking in circles toward affected side
How do you diagnose Listeria monocytogenes?
Culture of brain stem
T/F. you can vaccinate your herd against Listeria
F
Listeria monocytogene infection is caused by feeding poor-quality silage with a pH greater than ____.
5.5
T/F. Pasteruization can terminate Listeria monocytogenes
F
Gram positive small rods/filaments
Erysipelothrix
T/F. Erysipelothrix are non-motile facultative anaerobes
T
Are Erysipelothrix Catalase positive or negative?
Negative
Are Erysipelothrix oxidase positive or negative?
Negative
Where is Erysipelothrix commonly found in animals?
tonsils and intestines of mammals
Slime layer of fish
T/F Erysipelothrix are resistant to harsh environmental conditions
T
____ are the most important reservoir of Erysipelothrix.
swine
What 4 syndromes are observed in pigs infected with erysipelothrix?
- Septicemia
- Dermatopathy
- arthritis
- endocarditis
Production of _____ cleaves sialic acid on endothelial cell surface causing vascular damage and hyaline thrombus formation in animals infected with erysipelothrix.
Neuroaminidase
How is erysipelothrix excreted?
oronasal and fecal secretions
Virulence factor of Erysipeothrix that provides protection against phagocytosis and allows intracellular rep.
Polysaccharide capsule
Virulence factor of Erysipelothrix that allows for adherence and invasion of endothelial cells
Neuraminidase
Virulence factor of Erysipelothric that allows for dissemination of bacteria within tissues
Hyaluronidase
Virulence factor of Erysipelothrix that allows adhesion
SpaA surface protein
What are the 2 species of Erysipelothrix important in vet med?
- E rhusiopathiae
2. E. tonsallarum
Which type of Erysipelothrix infects dogs?
E. tonsallarum
Which species of Erysipelothric infects pigs, turkeys, and sheep
E rhusiopathiae
Erysipelothrix rhusiopathiae infection in pigs is known as?
swine erysipelas or Diamond skin disease
WHat are the 3 forms of swine erysipelas?
- acute
- subacute
- chronic
Pathognomic lesions of swine erysipelas
diamond shaped skin lesion
Why are pigs 3mo to 1 yr of age most susceptible to erysipelas infections?
< 3mo = protected by maternal ab.
>3yr protective active immunity through exposure
T/F. Erysipelas infections can be triggered by stress
T
What are 3 methods of diagnosing Erysipelas in swine
- skin lesions
- Isolation of E. rhusiopathiae
- PCR
Erysipelas in turkeys is cause by ____
Erysipelohrix rhusiopathiae
What is acute form Erysipelothric rhusiopathiae in turkeys characterized by?
septicemia and sudden death
What is chronic form Erysipelas in turkeys characterized by?
endocarditis and wt. loss
Non-suppurative polyarthritis in lambs is cause by infection of?
Erysipelothrix rhusiopathiae
How does Erysipelothrix rhusiopathiae organisms enter lambs?
through umbilicus or castration wounds
T/F. Humans can also get erysipelas?
T
Human erysipelas is an infectious skin disease cause by ________.
Group A beta-hemolytic streptococci