Exam 2: Borrelia, Brachyspira, and Treponema

Question 1

gram (-), loosely coiled spirochete which commonly cause arthropod borne infections

Answer 1

Borrelia

Question 2

T/F Boorrelia are rapid growing

Answer 2

F. slow

Question 3

T/F. Borrelia are microaerophilic

Answer 3

T

Question 4

T/F Borrelia have circular chromosomes

Answer 4

F Linear

Question 5

Which spp. of Borrelia cause lyme dz?

Answer 5

B. burgdorferi

Question 6

Which spp. of borrelia causes avian septicemic borreliosis

Answer 6

B. anserina

Question 7

Which spp. of Borrelia causes relapsing fever borrelia and how is it spread?

Answer 7

B. recurrentis, spread by human body louse

Question 8

which type of tick transmits lyme dz

Answer 8

ixodes scapularis

Question 9

Which spp can get lyme dz

Answer 9

humans, dogs, horses, cattle, shep

Question 10

after a tick ingests Lyme infected blood from an animal, spirochetes up regulate expression of _______, which is essential for virulence in mamals

Answer 10

Outer surface protein

Question 11

What is the main virulence factor of Borrelia

Answer 11

LPS

Question 12

What is the importance of LPS in borrelia spp.

Answer 12

Ability to hide out in L forms, spherplast, cyctic dormant form in the host

Question 13

where does borrilia burgdorferi LPS transform from Osp C to Osp A

Answer 13

In the midgut of tics

Question 14

WHere does B. brgdorferi LPS transform from Osp A to Osp C?

Answer 14

in the salivary gland of ticks

Question 15

What Osp is transferred from ticks to mammals?

Answer 15

Osp C

Question 16

T/F in the case of canine Lyme disease an antibody titer does not predict illness

Answer 16

T

Question 17

List some methods of Dx of Lyme Dz

Answer 17

1. direct detection in host tissue
2. Culture and isolation
3. Serology (SNAP), western blot, ELISA

Question 18

Tx for lyme dz

Answer 18

doxycycline or minocycline

Question 19

What are the two types of lyme vaccine?

Answer 19

1. OSPA

2. OSPC which kills tick when feeds

Question 20

Which spp. of Brachyspira causes swine dysentery in growing pigs?

Answer 20

B. hyodysenteriae

Question 21

WHich spp of Brachyspira causees Intestinal spirochetosis in animals and humans?

Answer 21

B. pilosicoli

Question 22

Which spps. of brachyspira have a controversial role in disease?

Answer 22

B. intermedia
B. mudochii
B. innocens
B. canis

Question 23

Anaerobic spirochete agent of swine dysentery

Answer 23

B. hyodysenteriae

Question 24

Where is B. hyodysenteriae an obligate pathogen?

Answer 24

swine colon (and mice)

Question 25

T/F B. hyodysenteriae survives well in the environment

Answer 25

F. poorly survives, but will survive in anaerobic environ.

Question 26

Where in the environment can Bracyspira hyodsenteriae survive?

Answer 26

anaerobic places

manure

Question 27

What are the virulence factors of Brachyspira hyodesenteriae

Answer 27

1. cytotoxin
2. Hemolysis
3. LPS

Question 28

What is the route of transmission of Brachyspira hyodesenteriae

Answer 28

Fecal - oral

Question 29

Where are lesions from Brachyspira hyodesenteriae found?

Answer 29

Lg intestine only

Question 30

How can you Dx Brachyspira hyodesenteriae

Answer 30

Direct staining
Anaerobic culture
PCR
Histopathology and silver staining

Question 31

Which spirochete causes syphilis?

Answer 31

Treponema pallidum

Question 32

T/F. Treponema can not be cultivated in the lab

Answer 32

T

Question 33

What is PDD

Answer 33

Papillomatous Digital dermatitis “Hairy heel warts” Treponema infection in cattle, causes lameness

Question 34

What is the Tx for PDD?

Answer 34

Spraying feet with abx, foraldehyde foot baths,

Question 35

What is Treponema paraluis- cuniculi?

Answer 35

Rabbit syphilis/ vent dz