Exam 1: General bacteriology Review Flashcards
T/F. Prokaryotes have no nucleus
T
T/F Bacteria are unicellular microorganisms
T
How do bacteria reproduce?
binary fission
Bacteria have a complex cell wall with a unique component called ______.
Peptidoglycan
What are the 3 groups of bacteria based on their unique cell wall components.
- Gram Positive bacteria
- Gram negative bacteria
- Mycobacteria
What is the unique cell wall component found in gram positive bacteria?
Lipoteichoic acid
What is the unique cell wall component found in gram negative bacteria?
Outer memb. containing lipopolysaccharide (LPS)
What is the unique cell wall component found in Mycobacteria?
Mycolic acid
What component of LPS can activate immune system and exert harmful effects on the host polysaccharide component contributes to antigenicity
Lipid A component
Gram positive stain ____
purple
Gram negative stain ____
pink
Describe why Gram positive bacteria stain purple
large crystal violet and grams iodine complexes cannot escape thick peptidoglycan layer
Describe why Gram negative bacteria stain pink.
picks up safranin because the thin peptidoglycan layer allows the passage of large crystal volet and grams iodine complexes
Acid Fast staining is due to the presence of _____.
mycolic acid
Acid fast positive bacteria have gram ____ cell wall
positive
Acid fast positive organisms stain ____
pink
Describe the staining of Acid fast positive organisms.
Retains the color of carbol fuchsin the primary stain b/c of the presence of mycolic acid in the cell wall
Acid fast negative organisms stain ____
blue
Describe the staining of Acid fast negative organisms.
get decolorized due to the absence of mycolic acid
Structure on the bacterial surface used in locomotion
Flagella
Small thread like structures on bacterial surface facilitate adherence to the host tissue through specific receptors
Pili/ fimbriae
Outer coating of the bacteria made of polysaccharide in most bacteria, help bacteria to evade phagocytosis
Capsule
Survival mech. for some gram pos. bacteria, spores are highly resistant dormant form of bacteria
Endospores
When are endospores produced?
when bacteria are exposed to adverse conditions and when essential nutrients are depleted