Exam 1: Gram Positive Streptococcus + Enterococcus Flashcards
What does streptococcus look like?
Gram + cocci in chains
T/F Streptococcus is spore forming.
F. Non spore forming
Is streptococcus catalase positive or negative
Positive
Where are streptococcus commensals?
on mucus membranes of the upper resp. tract and lower urogenital tract
T/F. Streptococcus do not survive well in the environment.
T. susceptible to desicccation
What are 3 ways to differentiate between streptococci species?
- Type of hemolysis
- Lancefield classification
- Biochemical testing
What are the 3 types of hemolysis used to differentiate streptococci?
- Beta-hemolytic
- Alpha- hemolytic
- Gamma- hemolytic
clear, complete hemolysis generally found in pyogenic spp.
Beta-hemolytic
Green, partial hemolysis found in S. suis and S. dysgalactiae
Alpha hemolysis
No hemolysis, found in enterococcus spp.
Gamma hemolytic
a serologic classification dividing hemolytic streptococci into groups (A-H) that bear a definite relationship to their sources, based on precipitation tests depending on group-specific carbohydrate substances
Lancefield classification
The Lancefield classification is based on cell wall polysaccharide antigen known as _____
C-substance
What are the two tests performed in the Lancefield classification?
- ring precipitation test
2. Latex agglutination test
T/F. Streptococci can be either Primary pathogenic infections or secondary infections.
T
What is an example of a primary streptococcal infection?
Strangles
____- hemolytic streptococci are generally more pathogenic
Beta
What are the 2 virulence factors of streptococci that are used for invasion?
- Streptokinase
2. Hyaluronidase
What is the virulence factor of streptococci that is used for adhesion?
Protein F
What are the 3 virulence factors of streptococci that are used for Evasion?
- M protein
- Hyaluronan
- Streptolysins
What are the 2 virulence factors of streptococci that are used as toxins?
- Streptococcal pyrogenic exotoxins
2. Lipoteichoic acid
What are the 7 streptococcal spp. that are of vet. importance?
- S. equi subsp. equi
- S. Suis
- S. agalactiae
- S. dysgalactiae
- S uberis
- S. canis
- S. equi. subsp. zooepidemicus
Equine infection caused by Streptococcus equi. subsp. equi
Strangles= Equine distemper
What is strangles?
highly contagious dz. cause by Streptococcus equi. subsp. equi, involving the upper resp. tract with abscessation of lymphnodes
T/F. Streptococcus equi subsp. equi is commensal
F
Horse comes into your clinic with: fever, depression, anorexia, followed by purulent oculonasal discharge. what do you expect?
Streptococcal equi subsp. equi
Which lymph nodes are affected in strangles?
submandibular and retropharyngeal lnn.
What are the major complication of Strangles?
- guttural pouch empyema
- Bastard Strangles
- Purpura haemorrhagica
- Asymptomatic carriers
What are 3 ways you can dx. strangles in horses?
- culturing from abscess
- Serology (ELISA)
- PCR
What protein are you looking for in you diagnosis’s?
M protein
What protein is found in vaccinations for strangles?
M protein
T/F. Strangles is a reportable disease?
T. in many states
Recognized worldwide as a cause of significant losses in the pig industry, associated with meningitis, arthritis, septicemia, and bronchopneumonia in pigs.
Streptococcus suis
What is another name for streptococcus suis?
Nursery dz
Pigs are natural carriers of S. suis in their ____
Tonsillar tissue
What is the predominant serotype worldwide of streptococcus suis?
Serotype 2
T/F Streptococcus suis is zoonotic
T
What 3 streptococcal species can cause bovine streptococcal mastitis?
- S. agalactiae
- S. dysgalactiae
- S. uberis
What are the 2 types of bovine streptococcal mastitis?
- contagious mastitis
2. environmental mastitis
What species of streptococcus causes contagious masttis?
S agalactiae
What two species of streptococcus causes environmental mastitis in cows?
- S. dysgalactiae
2. S. uberis
Treatment and preventionof bovine streptococcal mastitis?
- pre and post teat dipping
- reduce teat injury
- Dry cow treatment
- nutrition
- hygiene
- screen cow before entering herd
- monitor somatic cell count in milk
What species of streptococcus causes pyogenic streptococcus
- S equi subsp. zooepidemicus
- S. canis
- S. pyogenes
T/F. dogs can become infected with Streptococcus equi subsp. zooepidemicus
T. Dog, pig, horse, cow, sheep
What is the usual habitat of streptococcus equi subsp. zooepidemicus
mucous membranes
T/F Streptococcus equi subsp. zooepidemicus is zoonotic?
T. causes meningitis in humans
Streptococcal species that causes toxic shock syndrome and necrotizing fasciitis in dogs and cats
Streptococcus canis
T/F. Streptoccus canis is commensal?
T. commensal on skin and mucus membranes in cats and dogs
Streptococcus species that causes anything from mild superficial skin dz to life threatening flesh eating disease
S. pyogenes
Gram positive ovoids, often found in pairs or short chains
Enterococcus
T/F Enterococcus are anaerobes
F. facultative anaerobes
T/F. Enterococcus are motle
T. some strains are
T/F. Enterococcus are non spore forming
T
Are Enterococcus Catalase positive or negative
Negative
Where are Enterococcus commensal?
GIT
T/F. Enterococcus are opportunistic pathogens
T
Oooprtunistic infectionof enterococcus that can cause: wound infections mastitis in cattle UTI in dogs Ear infections in dogs Nosocomial infections
Enterococcus faecalis/ faecium
T/F Enterococcus are naturally resistant to many antimicrobials
T
Why are nosocomial infections of enterococcus important to us?
innate resistance to antimicrobial drugs