Enterobacteriaceae & Salmonella Flashcards
All Enterobacteriaceae are:
Gram stain?
Shape?
Catalase positive or negative?
Oxidase positive or negative?
Gram -
Rod shaped
Catalase positive
Oxidase negative
Enterobacteriaceae hide their cell wall (CW) by _____ _____
outer membrane
Enterobacteriaceae has ____ genera with a total of ____ species
68, 355
What can be seen here?
Enterobacteriaceae
Medically important eight bacterial genera
within Enterobacteriaceae include:
CEEK group is also called coliform bacteria b/c they ferment lactose.
The CEEK group are _______ fermentors
Which members of CEEK are Lactose positive?
lactose
Citrobacter, Enterobacter, Kiebsiella
SPY-Sh group are __________ ___-______.
Which members of SPY-Sh are Lactose negative?
lactose non-fermentors
Salomonella, yersinia, proteus
IMViC test mostly used in enterobac group. This is an abbreviation for the Indole test uses trytophan amino acid.
VP = ketone bodies, such as acetone
Which tests will help us determine the presence of Enterobacteriaceae?
Test for the presence of Lactose fermentation on MacConkey Agar.
Tests help us determine which bacteria cause disease in animals. These tests are:
1. Lactose fermentation (+/-)
2. H2S, CO2, or no as on TSA agar
3. Urease - if positive, they are proteins, if negative they are salmonella
If produce gas = citrobacter
If your MacConkey Agar turns pink, what does that mean?
Positive = pink colonies –> coliform bacteria
Which Enterobacteriaceae are considered to be “fast”?
Klebsiella, Enterobacter
Which Enterobacteriaceae are considered to be “slow”?
Citrobacter
If your MacConkey Agar turns pale, what does that mean?
Negative = pale colonies –> highly pathogenic bacteria.
You then test for H2S production on triple sugar iron (TSI).
Proteus and Salmonella are positive for?
H2S on triple sugar iron (TSI)
Proteus is Urease positive or negative?
positive
Salmonella is urease positive or negative?
negative
Yersinia is negative for ____ and _____, but positive for?
H2S, CO2, Ureas
Lactose positive bacteria are also called?
Coliform bacteria
Which Enterobacteriaceae are Indole positive?
Citrobacter
Which Enterobacteriaceae are Indole negative?
Klebsiella, Enterobacter, Salmonella, Yersinia, Proteus
Which bacteria are Voges-proskauer negative?
Citrobacter, Salmonella, Yersinia, Proteus (glucose)
Which bacteria are Voges-proskauer positive?
Klebsiella, enterobacter (acetoin)
Which Enterobacteriaceae are Citrate positive?
Yersinia
Proteus
Which Enterobacteriaceae are Citrate negative?
Salmonella
Klebsiella
Enterobacter
Citrobacter