chapter 5 (diana's version) Flashcards
microbial growth requirements:
Name the two categories:
physical and chemical
name the physical components of microbial growth
temperature
ph
pyschophiles
pyschotrophs
mesophiles
hyperthermaphiles
cidophiles alkaphiles
name the chemical components of microbial growth
carbon
oxygen
trace elements
nitrogen phosphorus sulfur iron
what is the temperature range min and max of pyschotrophs and it’s optimal temperature
min= 0
max= 35
optimal 15-30
name the temperature range and optimal temperature of mesophiles
temperature range= 10-45
optimal temp= 30-37
is protein very sensitive to temperature and acidic, our blood pH is 7.35
yes protein is very sensitive to temperature and acidic and it is a fact that our blood pH is 7.35
bacteria that grow at pH values between 5 and 8 are neutrophiles
yes this is true, and gastric acid is associated with the these mistakes and trying to heal so badly, you don’t want it in your stomach it can cause cancer.
is it true that cytoplasm will never ever become acidic
that is in fact true.
in a hypertonic environment , when water leaves what happens to the cell wall, and plasma membrane
when water leaves, the cell wall is rigid so it is not changing, the plasma membrane shrinks
is the cell dead in a hypertonic cell ? or is it not?
it is not dead in a hypertonic solution, it’s just waiting to give water
in a hypotonic solution, what does the cell wall contribute with the cell bursting?
the cell wall helps with the cell bursting by counterpressure
true or false. Fungi causes mold easily?
true
what is a mold?
it is a eukaryotic fungi
extreme halophiles why do they live in dead live sea?
because no one can rlly live through there, so they have decided to adapt and live there.
protien, nucleic acid, ATP are the fundamental results of nitrogen sulfur, phosphurus and iron ( they are required in smaller amounts then carbon), explain why?
they have these components in order to finalize the structure
Psychrophiles :
as soon as you eat the food, your body temperature is too high so they can’t grow in you.
true
how can hyperthermophiles living above boiling point?
because they are vegetative, they are metabolic producing enzymes
what happens to water when it freezes?
it freezes and its aggressive actually pokes hole in the cell
true or false.
Protein is not sensitive to temperature and acid.
false, they are highly sensitive
will a cytoplasm become acidic?
no it can never ever become acidic.
bacteria that grow at pH values between 5 and 8 are called what?
they are called neutrophiles
what is glactic acid, give a bit of description
glactic acid, waste product of milk , they become acidify, there proteins in your milk and unfold actually form chunk, and sour because they’ve been there (those bugs) they become acidic, that they can’t live there anymore.
neutrophiles what are they associated with?
they are associated with gstric ulcer, you don’t want it in your stomach , it causes cancer. It is associated with these mistakes, and trying to heal so badly that it leads to gastric ulcer.
what do you need to give a patient when you’re building up their blood volume?
you give them an isotonic solution.
when water leaves in a hypertonic solution, the cell wall is rigid, does it change at all?
no, it does not change at all.
in a hypotonic solution, we are aware that the cell burst, however, what helps with the bursting?
the cell wall helps with the bursting, with counterpressure
why does extreme halophiles enjoy 30% NaCl, that is extremely salty when we compare it to our blood which is only 0.9. Give a brief answer.
basically simple, because no other groups of bugs decide to live there, since its a free space, they love it.
what does anoxic and oxic mean?
anoxic mean no oxygen
oxic zone= with oxygen
what are we in terms of microorganisms based on oxygen use tolerance? and what is our muscle?
we are obligate aerobe, our muscle would be facultative muscle
what is pure culture?
pure culture is one strain and one bacterium only
true or false.
Agar can be solidified, that’s why we chose it, it’s hard (in terms of solid media)
true
name the terms with identifying these analogy:
A medium can be 2 things name them.
analogy: pre made cookie dough , you do not know what’s in there, you never know what you’re getting
Analogy: you know exactly what’s in there, this is reproducible, may not rich in growth, but can achieve the exact same result. Scratch cookie (making your own recipe)
- chemically undefined
2.chemically defined
if they add agar what is it, solid media or liquid media?
it is soli media, no agar defines liquid media
what does bile salt to your fats?
bile salts in selection of your intestinal media, they tear fats apart.
what is the example of both selective and differential
MacConkey Agar is both selective and differential
which of the following is an example of differential, and which is an example of selective?
- Bile salts and dyes in the media inhibit the growth of most non-intestinal bacteria, promoting the growth of bacteria that live in the intestine.
2.Lactose fermentation bacteria that ferment lactose sugar produce acid products that turn the pH indicator pink
- would be selective and 2. would be differential
true or false, Bacterial growth replicate and elongates at the same time?
true, this is why they take quick since its being done at the same timeq
most bacteria have a generation time in between what?
1-3 hours
e.coli takes about —–of generation time, what about M.tuberculosis?
e.coli takes 20 minutes and M.tuberculosis takes 24 hours
which one takes longer? viable or direct count?
viable count
