[51] Radioactive Tracers in Molecular Biology Flashcards

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1
Q

What is the principle of using Radioactive Tracers in Molecular Biology?

A

Radioactive tracers are atoms with unstable nuclei that are used to track and identify molecules in biological systems.

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2
Q

What are common isotopes used as Radioactive Tracers in Molecular Biology?

A
  • Carbon-14
  • Phosphorus-32
  • Sulfur-35
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3
Q

Initials: RT

A

Radioactive Tracers

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4
Q

What is the purpose of using Radioactive Tracers in Molecular Biology?

A

To follow the path of biomolecules in various biological processes.

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5
Q

How are Radioactive Tracers detected in Molecular Biology?

A

Using instruments like Geiger counters or scintillation counters.

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6
Q

Why are radioactive isotopes like Carbon-14 used in molecular biology?

A

Because they can be incorporated into organic molecules and tracked in biological processes.

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7
Q

What are the risks associated with using Radioactive Tracers?

A

Exposure to radioactive materials can cause damage to living tissues and increase cancer risk.

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8
Q

How can Radioactive Tracers be used in DNA sequencing?

A

By labeling the DNA nucleotides with radioactive tracers and using them to identify the order of base pairs.

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9
Q

What precautions are necessary when using Radioactive Tracers?

A

Proper handling and disposal methods must be followed to minimize exposure.

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10
Q

What are some applications of Radioactive Tracers in Molecular Biology?

A
  • Studying metabolic pathways
  • DNA sequencing
  • Protein labeling
  • Drug tracing in pharmacology
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