[35] Fungal Cell Culture Flashcards
What is a Fungal Cell Culture?
A method used to grow and maintain fungal cells in a controlled laboratory environment.
What is the main purpose of Fungal Cell Culture?
For the study of fungi, production of fungal metabolites, or genetic manipulation.
What are the basic requirements for a Fungal Cell Culture?
- Suitable growth medium
- Appropriate temperature
- Proper pH
- Suitable atmosphere
How does temperature affect Fungal Cell Culture?
Different fungi have different optimal growth temperatures. Most grow well at room temperature.
How does pH affect Fungal Cell Culture?
It influences enzyme activity and protein stability. Most fungi prefer slightly acidic conditions.
What is a growth medium in Fungal Cell Culture?
A mixture of nutrients that supports the growth of fungi.
What is a commonly used medium in Fungal Cell Culture?
Sabouraud Dextrose Agar (SDA).
What is SDA?
Sabouraud Dextrose Agar, a type of growth medium used in fungal culture.
How long can a Fungal Cell Culture typically take?
From a few days to several weeks, depending on the fungal species.
What is incubation in the context of Fungal Cell Culture?
The process of keeping fungal cultures at a specific temperature to allow growth.
Why is sterility important in Fungal Cell Culture?
To prevent contamination by bacteria or other fungi.
What is sub-culturing in Fungal Cell Culture?
The process of transferring fungi from a previous culture into fresh growth medium to prolong the life of the culture.
What is a pure culture in Fungal Cell Culture?
A culture containing a single species of organism.
What are the typical observations in a successful Fungal Cell Culture?
Visible fungal growth, usually as colonies or filaments.
What precautions are necessary when handling Fungal Cell Cultures?
Use of personal protective equipment, working in sterile conditions, and proper disposal of used cultures.
What are fungal metabolites?
Biochemicals produced by fungi which can have various applications in medicine, industry, and research.
What are the applications of Fungal Cell Culture?
In microbiology research, industry (for production of enzymes, antibiotics), medical diagnostics, and more.
What are some examples of fungi commonly grown in culture?
Aspergillus, Penicillium, Candida, and more.
Why is the choice of growth medium important in Fungal Cell Culture?
Different fungi require different nutrients, influencing their growth and metabolite production.
How is fungal growth typically measured in Fungal Cell Culture?
By changes in turbidity, colony count, or dry weight of the culture.
